ANAC032 regulates root growth through the MYB30 gene regulatory network

Abstract Reactive oxygen species (ROS) play important roles as root growth regulators. We previously reported a comprehensive transcriptomic atlas, which we named ROS-map, that revealed ROS-responsible genes in Arabidopsis root tips. By using ROS-map, we have characterised an early ROS response key...

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Autores principales:	Hiromasa Maki, Satomi Sakaoka, Tomotaka Itaya, Takamasa Suzuki, Kaho Mabuchi, Takashi Amabe, Nobutaka Suzuki, Tetsuya Higashiyama, Yasuomi Tada, Tsuyoshi Nakagawa, Atsushi Morikami, Hironaka Tsukagoshi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0d6b25cc88b427fa0ce43ee5a900951
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spelling	oai:doaj.org-article:e0d6b25cc88b427fa0ce43ee5a9009512021-12-02T15:08:32ZANAC032 regulates root growth through the MYB30 gene regulatory network10.1038/s41598-019-47822-02045-2322https://doaj.org/article/e0d6b25cc88b427fa0ce43ee5a9009512019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47822-0https://doaj.org/toc/2045-2322Abstract Reactive oxygen species (ROS) play important roles as root growth regulators. We previously reported a comprehensive transcriptomic atlas, which we named ROS-map, that revealed ROS-responsible genes in Arabidopsis root tips. By using ROS-map, we have characterised an early ROS response key transcription factor, MYB30, as a regulator of root cell elongation under ROS signals. However, there are other ROS-responsible transcription factors which have the potential to regulate root growth. In the present study, we characterised the function of another early ROS-responsible transcription factor, ANAC032, that was selected from ROS-map. Overexpression of ANAC032 fused with the transcriptional activation domain, VP16, inhibited root growth, especially decreasing cell elongation. By transcriptome analysis, we revealed that ANAC032 regulated many stress-responsible genes in the roots. Intriguingly, ANAC032 upregulated MYB30 and its target genes. The upregulation of MYB30 target genes was completely abolished in the ANAC032-VP16x2 OX and ANAC032 estradiol-inducible line in myb30-2 mutants. Moreover, root growth inhibition was alleviated in ANAC032-OX in myb30-2 mutants. Overall, we characterised an upstream transcription factor, ANAC032, of the MYB30 transcriptional cascade which is a key regulator for root cell elongation under ROS signalling.Hiromasa MakiSatomi SakaokaTomotaka ItayaTakamasa SuzukiKaho MabuchiTakashi AmabeNobutaka SuzukiTetsuya HigashiyamaYasuomi TadaTsuyoshi NakagawaAtsushi MorikamiHironaka TsukagoshiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hiromasa Maki Satomi Sakaoka Tomotaka Itaya Takamasa Suzuki Kaho Mabuchi Takashi Amabe Nobutaka Suzuki Tetsuya Higashiyama Yasuomi Tada Tsuyoshi Nakagawa Atsushi Morikami Hironaka Tsukagoshi ANAC032 regulates root growth through the MYB30 gene regulatory network
description	Abstract Reactive oxygen species (ROS) play important roles as root growth regulators. We previously reported a comprehensive transcriptomic atlas, which we named ROS-map, that revealed ROS-responsible genes in Arabidopsis root tips. By using ROS-map, we have characterised an early ROS response key transcription factor, MYB30, as a regulator of root cell elongation under ROS signals. However, there are other ROS-responsible transcription factors which have the potential to regulate root growth. In the present study, we characterised the function of another early ROS-responsible transcription factor, ANAC032, that was selected from ROS-map. Overexpression of ANAC032 fused with the transcriptional activation domain, VP16, inhibited root growth, especially decreasing cell elongation. By transcriptome analysis, we revealed that ANAC032 regulated many stress-responsible genes in the roots. Intriguingly, ANAC032 upregulated MYB30 and its target genes. The upregulation of MYB30 target genes was completely abolished in the ANAC032-VP16x2 OX and ANAC032 estradiol-inducible line in myb30-2 mutants. Moreover, root growth inhibition was alleviated in ANAC032-OX in myb30-2 mutants. Overall, we characterised an upstream transcription factor, ANAC032, of the MYB30 transcriptional cascade which is a key regulator for root cell elongation under ROS signalling.
format	article
author	Hiromasa Maki Satomi Sakaoka Tomotaka Itaya Takamasa Suzuki Kaho Mabuchi Takashi Amabe Nobutaka Suzuki Tetsuya Higashiyama Yasuomi Tada Tsuyoshi Nakagawa Atsushi Morikami Hironaka Tsukagoshi
author_facet	Hiromasa Maki Satomi Sakaoka Tomotaka Itaya Takamasa Suzuki Kaho Mabuchi Takashi Amabe Nobutaka Suzuki Tetsuya Higashiyama Yasuomi Tada Tsuyoshi Nakagawa Atsushi Morikami Hironaka Tsukagoshi
author_sort	Hiromasa Maki
title	ANAC032 regulates root growth through the MYB30 gene regulatory network
title_short	ANAC032 regulates root growth through the MYB30 gene regulatory network
title_full	ANAC032 regulates root growth through the MYB30 gene regulatory network
title_fullStr	ANAC032 regulates root growth through the MYB30 gene regulatory network
title_full_unstemmed	ANAC032 regulates root growth through the MYB30 gene regulatory network
title_sort	anac032 regulates root growth through the myb30 gene regulatory network
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e0d6b25cc88b427fa0ce43ee5a900951
work_keys_str_mv	AT hiromasamaki anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT satomisakaoka anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT tomotakaitaya anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT takamasasuzuki anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT kahomabuchi anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT takashiamabe anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT nobutakasuzuki anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT tetsuyahigashiyama anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT yasuomitada anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT tsuyoshinakagawa anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT atsushimorikami anac032regulatesrootgrowththroughthemyb30generegulatorynetwork AT hironakatsukagoshi anac032regulatesrootgrowththroughthemyb30generegulatorynetwork
_version_	1718388095070502912

ANAC032 regulates root growth through the MYB30 gene regulatory network

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