Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.

Escherichia coli G3/10 is a component of the probiotic drug Symbioflor 2. In an in vitro assay with human intestinal epithelial cells, E. coli G3/10 is capable of suppressing adherence of enteropathogenic E. coli E2348/69. In this study, we demonstrate that a completely novel class II microcin, prod...

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Autores principales:	Anke Zschüttig, Kurt Zimmermann, Jochen Blom, Alexander Goesmann, Christoph Pöhlmann, Florian Gunzer
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0e276b5af514a888fc888072d06af76
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spelling	oai:doaj.org-article:e0e276b5af514a888fc888072d06af762021-11-18T07:23:43ZIdentification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.1932-620310.1371/journal.pone.0033351https://doaj.org/article/e0e276b5af514a888fc888072d06af762012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22479389/?tool=EBIhttps://doaj.org/toc/1932-6203Escherichia coli G3/10 is a component of the probiotic drug Symbioflor 2. In an in vitro assay with human intestinal epithelial cells, E. coli G3/10 is capable of suppressing adherence of enteropathogenic E. coli E2348/69. In this study, we demonstrate that a completely novel class II microcin, produced by probiotic E. coli G3/10, is responsible for this behavior. We named this antibacterial peptide microcin S (MccS). Microcin S is coded on a 50.6 kb megaplasmid of E. coli G3/10, which we have completely sequenced and annotated. The microcin S operon is about 4.7 kb in size and is comprised of four genes. Subcloning of the genes and gene fragments followed by gene expression experiments enabled us to functionally characterize all members of this operon, and to clearly identify the nucleotide sequences encoding the microcin itself (mcsS), its transport apparatus and the gene mcsI conferring self immunity against microcin S. Overexpression of cloned mcsI antagonizes MccS activity, thus protecting indicator strain E. coli E2348/69 in the in vitro adherence assay. Moreover, growth of E. coli transformed with a plasmid containing mcsS under control of an araC PBAD activator-promoter is inhibited upon mcsS induction. Our data provide further mechanistic insight into the probiotic behavior of E. coli G3/10.Anke ZschüttigKurt ZimmermannJochen BlomAlexander GoesmannChristoph PöhlmannFlorian GunzerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33351 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anke Zschüttig Kurt Zimmermann Jochen Blom Alexander Goesmann Christoph Pöhlmann Florian Gunzer Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.
description	Escherichia coli G3/10 is a component of the probiotic drug Symbioflor 2. In an in vitro assay with human intestinal epithelial cells, E. coli G3/10 is capable of suppressing adherence of enteropathogenic E. coli E2348/69. In this study, we demonstrate that a completely novel class II microcin, produced by probiotic E. coli G3/10, is responsible for this behavior. We named this antibacterial peptide microcin S (MccS). Microcin S is coded on a 50.6 kb megaplasmid of E. coli G3/10, which we have completely sequenced and annotated. The microcin S operon is about 4.7 kb in size and is comprised of four genes. Subcloning of the genes and gene fragments followed by gene expression experiments enabled us to functionally characterize all members of this operon, and to clearly identify the nucleotide sequences encoding the microcin itself (mcsS), its transport apparatus and the gene mcsI conferring self immunity against microcin S. Overexpression of cloned mcsI antagonizes MccS activity, thus protecting indicator strain E. coli E2348/69 in the in vitro adherence assay. Moreover, growth of E. coli transformed with a plasmid containing mcsS under control of an araC PBAD activator-promoter is inhibited upon mcsS induction. Our data provide further mechanistic insight into the probiotic behavior of E. coli G3/10.
format	article
author	Anke Zschüttig Kurt Zimmermann Jochen Blom Alexander Goesmann Christoph Pöhlmann Florian Gunzer
author_facet	Anke Zschüttig Kurt Zimmermann Jochen Blom Alexander Goesmann Christoph Pöhlmann Florian Gunzer
author_sort	Anke Zschüttig
title	Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.
title_short	Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.
title_full	Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.
title_fullStr	Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.
title_full_unstemmed	Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.
title_sort	identification and characterization of microcin s, a new antibacterial peptide produced by probiotic escherichia coli g3/10.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e0e276b5af514a888fc888072d06af76
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Identification and characterization of microcin S, a new antibacterial peptide produced by probiotic Escherichia coli G3/10.

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