pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors

Abstract Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient’s disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the de...

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Autores principales:	Rashmi Chaudhari, Abhijeet Joshi, Rohit Srivastava
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0e5a87107164eb4a108a5447d7d26ee
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spelling	oai:doaj.org-article:e0e5a87107164eb4a108a5447d7d26ee2021-12-02T11:40:50ZpH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors10.1038/s41598-017-06060-y2045-2322https://doaj.org/article/e0e5a87107164eb4a108a5447d7d26ee2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06060-yhttps://doaj.org/toc/2045-2322Abstract Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient’s disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the development of single fluorophore and dual fluorophore ratiometric biosensors based on alginate microspheres for pH and urea analysis in urine samples. A facile method of air driven atomization was used for developing these polymeric fluorophore and enzyme based biosensors. Ratiometric biosensors were developed using layer-by-layer coating of polyelectrolyte conjugated to reference fluorophores. Biosensing studies using these biosensors showed that samples in pathophysiological range can be measured having pH range of 4–8 and urea levels between 0–50 mM. Testing of urine samples using these biosensors showed that both pH and urea detection can be accurately performed without interference. Thus, we believe that FITC-Dextran and FITC-Dextran/RuBpy based pH and urea biosensors show a great potential to be translated as a point of care device for pH and urea biosensing in early detection and continuous monitoring of kidney diseases.Rashmi ChaudhariAbhijeet JoshiRohit SrivastavaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rashmi Chaudhari Abhijeet Joshi Rohit Srivastava pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors
description	Abstract Kidney diseases remain often undiagnosed due to inefficient screening methods available at patient’s disposal. Early diagnosis and effective management of kidney problems can best be addressed by the development of biosensors for commonly occurring clinical biomarkers. Here we report the development of single fluorophore and dual fluorophore ratiometric biosensors based on alginate microspheres for pH and urea analysis in urine samples. A facile method of air driven atomization was used for developing these polymeric fluorophore and enzyme based biosensors. Ratiometric biosensors were developed using layer-by-layer coating of polyelectrolyte conjugated to reference fluorophores. Biosensing studies using these biosensors showed that samples in pathophysiological range can be measured having pH range of 4–8 and urea levels between 0–50 mM. Testing of urine samples using these biosensors showed that both pH and urea detection can be accurately performed without interference. Thus, we believe that FITC-Dextran and FITC-Dextran/RuBpy based pH and urea biosensors show a great potential to be translated as a point of care device for pH and urea biosensing in early detection and continuous monitoring of kidney diseases.
format	article
author	Rashmi Chaudhari Abhijeet Joshi Rohit Srivastava
author_facet	Rashmi Chaudhari Abhijeet Joshi Rohit Srivastava
author_sort	Rashmi Chaudhari
title	pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors
title_short	pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors
title_full	pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors
title_fullStr	pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors
title_full_unstemmed	pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors
title_sort	ph and urea estimation in urine samples using single fluorophore and ratiometric fluorescent biosensors
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e0e5a87107164eb4a108a5447d7d26ee
work_keys_str_mv	AT rashmichaudhari phandureaestimationinurinesamplesusingsinglefluorophoreandratiometricfluorescentbiosensors AT abhijeetjoshi phandureaestimationinurinesamplesusingsinglefluorophoreandratiometricfluorescentbiosensors AT rohitsrivastava phandureaestimationinurinesamplesusingsinglefluorophoreandratiometricfluorescentbiosensors
_version_	1718395552186499072

pH and Urea Estimation in Urine Samples using Single Fluorophore and Ratiometric Fluorescent Biosensors

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