Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study
Giulia Collodel,1 Antonella Fioravanti,2 Nicola Antonio Pascarelli,2 Antonello Lamboglia,2 Vania Fontani,3 Margherita Maioli,3–5 Sara Santaniello,4,5 Gianfranco Pigliaru,4,5 Alessandro Castagna,3 Elena Moretti,1 Francesca Iacoponi,1 Salvatore Rinaldi,3 Carlo Ventura3,5,6 1Department of Bio...
Guardado en:
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Dove Medical Press
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e0ee9304344844e4826f8a396b781754 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e0ee9304344844e4826f8a396b781754 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e0ee9304344844e4826f8a396b7817542021-12-02T04:42:03ZEffects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study1178-1998https://doaj.org/article/e0ee9304344844e4826f8a396b7817542013-03-01T00:00:00Zhttps://www.dovepress.com/effects-of-regenerative-radioelectric-asymmetric-conveyer-treatment-on-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Giulia Collodel,1 Antonella Fioravanti,2 Nicola Antonio Pascarelli,2 Antonello Lamboglia,2 Vania Fontani,3 Margherita Maioli,3–5 Sara Santaniello,4,5 Gianfranco Pigliaru,4,5 Alessandro Castagna,3 Elena Moretti,1 Francesca Iacoponi,1 Salvatore Rinaldi,3 Carlo Ventura3,5,6 1Department of Biomedical Sciences (Applied Biology), 2Department of Clinical Medicine and Immunological Sciences (Rheumatology Unit), University of Siena, Siena, Italy; 3Department of Regenerative Medicine, Rinaldi Fontani Institute, Florence, Italy; 4Department of Biomedical Sciences, University of Sassari, Sassari, Italy; 5Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems, Bologna, Italy; 6Cardiovascular Department, S Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy Purpose: Osteoarthritis (OA) is a degenerative disease characterized by a progressive loss of articular cartilage extracellular matrix and is due to functional impairments occurring in chondrocytes. In previous works, we highlighted that Regenerative Tissue Optimization (TO-RGN) treatment with radioelectric asymmetric conveyer (REAC) technology influenced the gene expression profiles controlling stem cell differentiation and the pluripotency of human skin-derived fibroblasts in vitro. Since interleukin-1 beta signaling has been implicated in the induction and progression of this disease (through metalloproteinase-3 synthesis and nitric oxide production), we investigated whether REAC TO-RGN might influence the biochemical and morphological changes induced by interleukin-1 beta in normal and OA chondrocytes. Methods: The induction of metalloproteinase-3 and proteoglycan synthesis was evaluated by a solid-phase enzyme-amplified sensitivity immunoassay, and nitric oxide production was evaluated with the Griess method. Ultrastructural features were observed by transmission electron microscopy. Results: REAC TO-RGN treatment decreased nitric oxide and metalloproteinase-3 production in normal and OA chondrocytes, while inducing an increase in proteoglycan synthesis. OA chondrocytes were more affected by REAC TO-RGN treatment than were normal chondrocytes. Ultrastructural changes confirmed that REAC TO-RGN may counteract the negative effects of interleukin-1 beta incubation. Conclusion: The results of this in vitro study suggest that REAC TO-RGN treatment may represent a new, promising approach for the management of OA. Keywords: human chondrocytes ultrastructure, metalloproteinase, nitric oxide, proteoglycans, REAC TO-RGN treatmentCollodel GFioravanti APascarelli NALamboglia AFontani VMaioli MSantaniello SPigliaru GCastagna AMoretti EIacoponi FRinaldi SVentura CDove Medical Pressarticlehuman chondrocytes ultrastructuremetalloproteinasenitric oxideproteoglycansREAC TO-RGN treatmentGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 8, Pp 309-316 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
human chondrocytes ultrastructure metalloproteinase nitric oxide proteoglycans REAC TO-RGN treatment Geriatrics RC952-954.6 |
| spellingShingle |
human chondrocytes ultrastructure metalloproteinase nitric oxide proteoglycans REAC TO-RGN treatment Geriatrics RC952-954.6 Collodel G Fioravanti A Pascarelli NA Lamboglia A Fontani V Maioli M Santaniello S Pigliaru G Castagna A Moretti E Iacoponi F Rinaldi S Ventura C Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study |
| description |
Giulia Collodel,1 Antonella Fioravanti,2 Nicola Antonio Pascarelli,2 Antonello Lamboglia,2 Vania Fontani,3 Margherita Maioli,3–5 Sara Santaniello,4,5 Gianfranco Pigliaru,4,5 Alessandro Castagna,3 Elena Moretti,1 Francesca Iacoponi,1 Salvatore Rinaldi,3 Carlo Ventura3,5,6 1Department of Biomedical Sciences (Applied Biology), 2Department of Clinical Medicine and Immunological Sciences (Rheumatology Unit), University of Siena, Siena, Italy; 3Department of Regenerative Medicine, Rinaldi Fontani Institute, Florence, Italy; 4Department of Biomedical Sciences, University of Sassari, Sassari, Italy; 5Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems, Bologna, Italy; 6Cardiovascular Department, S Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy Purpose: Osteoarthritis (OA) is a degenerative disease characterized by a progressive loss of articular cartilage extracellular matrix and is due to functional impairments occurring in chondrocytes. In previous works, we highlighted that Regenerative Tissue Optimization (TO-RGN) treatment with radioelectric asymmetric conveyer (REAC) technology influenced the gene expression profiles controlling stem cell differentiation and the pluripotency of human skin-derived fibroblasts in vitro. Since interleukin-1 beta signaling has been implicated in the induction and progression of this disease (through metalloproteinase-3 synthesis and nitric oxide production), we investigated whether REAC TO-RGN might influence the biochemical and morphological changes induced by interleukin-1 beta in normal and OA chondrocytes. Methods: The induction of metalloproteinase-3 and proteoglycan synthesis was evaluated by a solid-phase enzyme-amplified sensitivity immunoassay, and nitric oxide production was evaluated with the Griess method. Ultrastructural features were observed by transmission electron microscopy. Results: REAC TO-RGN treatment decreased nitric oxide and metalloproteinase-3 production in normal and OA chondrocytes, while inducing an increase in proteoglycan synthesis. OA chondrocytes were more affected by REAC TO-RGN treatment than were normal chondrocytes. Ultrastructural changes confirmed that REAC TO-RGN may counteract the negative effects of interleukin-1 beta incubation. Conclusion: The results of this in vitro study suggest that REAC TO-RGN treatment may represent a new, promising approach for the management of OA. Keywords: human chondrocytes ultrastructure, metalloproteinase, nitric oxide, proteoglycans, REAC TO-RGN treatment |
| format |
article |
| author |
Collodel G Fioravanti A Pascarelli NA Lamboglia A Fontani V Maioli M Santaniello S Pigliaru G Castagna A Moretti E Iacoponi F Rinaldi S Ventura C |
| author_facet |
Collodel G Fioravanti A Pascarelli NA Lamboglia A Fontani V Maioli M Santaniello S Pigliaru G Castagna A Moretti E Iacoponi F Rinaldi S Ventura C |
| author_sort |
Collodel G |
| title |
Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study |
| title_short |
Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study |
| title_full |
Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study |
| title_fullStr |
Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study |
| title_full_unstemmed |
Effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to IL-1β. A biochemical and morphological study |
| title_sort |
effects of regenerative radioelectric asymmetric conveyer treatment on human normal and osteoarthritic chondrocytes exposed to il-1β. a biochemical and morphological study |
| publisher |
Dove Medical Press |
| publishDate |
2013 |
| url |
https://doaj.org/article/e0ee9304344844e4826f8a396b781754 |
| work_keys_str_mv |
AT collodelg effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT fioravantia effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT pascarellina effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT lambogliaa effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT fontaniv effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT maiolim effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT santaniellos effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT pigliarug effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT castagnaa effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT morettie effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT iacoponif effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT rinaldis effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy AT venturac effectsofregenerativeradioelectricasymmetricconveyertreatmentonhumannormalandosteoarthriticchondrocytesexposedtoil1betaabiochemicalandmorphologicalstudy |
| _version_ |
1718401156801101824 |