The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways

ABSTRACT Oxidation of aromatic compounds can be mutagenic due to the accumulation of reactive oxygen species (ROS) in bacterial cells and thereby facilitate evolution of corresponding catabolic pathways. To examine the effect of the background biochemical network on the evolvability of environmental...

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Autor principal:	Maia Kivisaar
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Pseudomonas putida bacterial evolution biodegradation cellular redox status mutation frequency oxidative stress Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e0efdb660e8a44f4b34164243e3daf3d
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id	oai:doaj.org-article:e0efdb660e8a44f4b34164243e3daf3d
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spelling	oai:doaj.org-article:e0efdb660e8a44f4b34164243e3daf3d2021-11-15T15:58:21ZThe Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways10.1128/mBio.01981-182150-7511https://doaj.org/article/e0efdb660e8a44f4b34164243e3daf3d2018-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01981-18https://doaj.org/toc/2150-7511ABSTRACT Oxidation of aromatic compounds can be mutagenic due to the accumulation of reactive oxygen species (ROS) in bacterial cells and thereby facilitate evolution of corresponding catabolic pathways. To examine the effect of the background biochemical network on the evolvability of environmental bacteria hosting a new catabolic pathway, Akkaya and colleagues (mBio 9:e01512-18, 2018, https://doi.org/10.1128/mBio.01512-18) introduced the still-evolving 2,4-dinitrotoluene (2,4-DNT) pathway genes from the original environmental Burkholderia sp. isolate into the genome of Pseudomonas putida KT2440. They show that the mutagenic effect of 2,4-DNT oxidation, which is associated with the accumulation of ROS and oxidative damage on DNA, can be avoided by preserving high NADPH levels in P. putida. The observations of this study highlight the impact of the cellular redox status of bacteria on the evolvability of new metabolic pathways.Maia KivisaarAmerican Society for MicrobiologyarticlePseudomonas putidabacterial evolutionbiodegradationcellular redox statusmutation frequencyoxidative stressMicrobiologyQR1-502ENmBio, Vol 9, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pseudomonas putida bacterial evolution biodegradation cellular redox status mutation frequency oxidative stress Microbiology QR1-502
spellingShingle	Pseudomonas putida bacterial evolution biodegradation cellular redox status mutation frequency oxidative stress Microbiology QR1-502 Maia Kivisaar The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways
description	ABSTRACT Oxidation of aromatic compounds can be mutagenic due to the accumulation of reactive oxygen species (ROS) in bacterial cells and thereby facilitate evolution of corresponding catabolic pathways. To examine the effect of the background biochemical network on the evolvability of environmental bacteria hosting a new catabolic pathway, Akkaya and colleagues (mBio 9:e01512-18, 2018, https://doi.org/10.1128/mBio.01512-18) introduced the still-evolving 2,4-dinitrotoluene (2,4-DNT) pathway genes from the original environmental Burkholderia sp. isolate into the genome of Pseudomonas putida KT2440. They show that the mutagenic effect of 2,4-DNT oxidation, which is associated with the accumulation of ROS and oxidative damage on DNA, can be avoided by preserving high NADPH levels in P. putida. The observations of this study highlight the impact of the cellular redox status of bacteria on the evolvability of new metabolic pathways.
format	article
author	Maia Kivisaar
author_facet	Maia Kivisaar
author_sort	Maia Kivisaar
title	The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways
title_short	The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways
title_full	The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways
title_fullStr	The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways
title_full_unstemmed	The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways
title_sort	effect of cellular redox status on the evolvability of new catabolic pathways
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/e0efdb660e8a44f4b34164243e3daf3d
work_keys_str_mv	AT maiakivisaar theeffectofcellularredoxstatusontheevolvabilityofnewcatabolicpathways AT maiakivisaar effectofcellularredoxstatusontheevolvabilityofnewcatabolicpathways
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The Effect of Cellular Redox Status on the Evolvability of New Catabolic Pathways

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