Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines

Preparations such as XilaVet, Zoletil 100 as well as Aeranne (Isoflurane) are successfully applied for animal anesthesia in veterinary practice. We assessed a possibility of using parenteral narcosis with Zoletil 100 in combination with muscle relaxant Xila for producer-rabbits involved in manufactu...

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Autores principales:	A. P. Semakova, V. G. Germanchuk, V. V. Rogozhin, N. Y. Shavina, M. V. Ovchinnikova, T. Y. Kirilova, L. F. Livanova, N. I. Belyakova
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	anesthesia analgesia inhalation narcosis laboratory animals experiment immunobiological preparations Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e0f287de53464a7b9322d6851ef926da
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spelling	oai:doaj.org-article:e0f287de53464a7b9322d6851ef926da2021-11-22T07:09:54ZAnesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines2220-76192313-739810.15789/2220-7619-AOL-810https://doaj.org/article/e0f287de53464a7b9322d6851ef926da2020-04-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/810https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Preparations such as XilaVet, Zoletil 100 as well as Aeranne (Isoflurane) are successfully applied for animal anesthesia in veterinary practice. We assessed a possibility of using parenteral narcosis with Zoletil 100 in combination with muscle relaxant Xila for producer-rabbits involved in manufacturing of natural rabbit serum subsequently deployed for production of diagnostic serum and immunoglobulin preparations. Administration of preparations into auricular vein is easy to do, while animals are sedated immediately allowing for safe fixation on restraining table and causing no additional stress for biomodels. This type of narcosis provides for expected depth of anesthesia and its maintenance until the end of blood-letting procedure. Parameters characterizing the state of cardiovascular system due to anesthetic products remained within the permitted limits. These preparations do not reduce heart beat rate allowing for collecting sufficient blood volumes. Application of inhalation anesthesia with Aeranne in laboratory animals provides for the specified depth of anesthesia and its maintenance until the end of the whole procedure. However, it requires specialized equipment and highly trained personnel with appropriate skills. Usage of Xila as a mono narcosis is not recommended as exhibits weak analgesic effects and strong hypotensive activity by decreasing quantity of collected blood volume. It was found that anesthetics such as Xila, Zoletil 100, Aeranne did not affect specific activity of immune sera in case of total dehematizing procedure. Moreover, antibody titers were not declined throughout entire observation (12 months) period and complied with the requirements of regulatory documentation. In addition, a feasibility of replacing old-fashioned anesthesia method with diethyl ether for a combination of safer contemporary preparations of Zoletil 100 and Xila was demonstrated while manufacturing tableted chemical cholera vaccine in experimental series with suckling rabbits used at diverse stages of raw material verification during surgical interventions. Xila, Zoletil 100, and Aeranne examined by us had no impact on the amount of blood obtained from donor-animals, immunological properties of the sera and ready-to-use diagnostic preparations. Such drugs were safe for all-age animals that comply with the requirements to anesthesia of animal biomodels and producer-animals in manufacturing of immunobiological preparations. Thus, our study allowed to conduct experiments with laboratory animals in a more humane manner.A. P. SemakovaV. G. GermanchukV. V. RogozhinN. Y. ShavinaM. V. OvchinnikovaT. Y. KirilovaL. F. LivanovaN. I. BelyakovaSankt-Peterburg : NIIÈM imeni Pasteraarticleanesthesiaanalgesiainhalation narcosislaboratory animalsexperimentimmunobiological preparationsInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 10, Iss 1, Pp 83-89 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	anesthesia analgesia inhalation narcosis laboratory animals experiment immunobiological preparations Infectious and parasitic diseases RC109-216
spellingShingle	anesthesia analgesia inhalation narcosis laboratory animals experiment immunobiological preparations Infectious and parasitic diseases RC109-216 A. P. Semakova V. G. Germanchuk V. V. Rogozhin N. Y. Shavina M. V. Ovchinnikova T. Y. Kirilova L. F. Livanova N. I. Belyakova Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
description	Preparations such as XilaVet, Zoletil 100 as well as Aeranne (Isoflurane) are successfully applied for animal anesthesia in veterinary practice. We assessed a possibility of using parenteral narcosis with Zoletil 100 in combination with muscle relaxant Xila for producer-rabbits involved in manufacturing of natural rabbit serum subsequently deployed for production of diagnostic serum and immunoglobulin preparations. Administration of preparations into auricular vein is easy to do, while animals are sedated immediately allowing for safe fixation on restraining table and causing no additional stress for biomodels. This type of narcosis provides for expected depth of anesthesia and its maintenance until the end of blood-letting procedure. Parameters characterizing the state of cardiovascular system due to anesthetic products remained within the permitted limits. These preparations do not reduce heart beat rate allowing for collecting sufficient blood volumes. Application of inhalation anesthesia with Aeranne in laboratory animals provides for the specified depth of anesthesia and its maintenance until the end of the whole procedure. However, it requires specialized equipment and highly trained personnel with appropriate skills. Usage of Xila as a mono narcosis is not recommended as exhibits weak analgesic effects and strong hypotensive activity by decreasing quantity of collected blood volume. It was found that anesthetics such as Xila, Zoletil 100, Aeranne did not affect specific activity of immune sera in case of total dehematizing procedure. Moreover, antibody titers were not declined throughout entire observation (12 months) period and complied with the requirements of regulatory documentation. In addition, a feasibility of replacing old-fashioned anesthesia method with diethyl ether for a combination of safer contemporary preparations of Zoletil 100 and Xila was demonstrated while manufacturing tableted chemical cholera vaccine in experimental series with suckling rabbits used at diverse stages of raw material verification during surgical interventions. Xila, Zoletil 100, and Aeranne examined by us had no impact on the amount of blood obtained from donor-animals, immunological properties of the sera and ready-to-use diagnostic preparations. Such drugs were safe for all-age animals that comply with the requirements to anesthesia of animal biomodels and producer-animals in manufacturing of immunobiological preparations. Thus, our study allowed to conduct experiments with laboratory animals in a more humane manner.
format	article
author	A. P. Semakova V. G. Germanchuk V. V. Rogozhin N. Y. Shavina M. V. Ovchinnikova T. Y. Kirilova L. F. Livanova N. I. Belyakova
author_facet	A. P. Semakova V. G. Germanchuk V. V. Rogozhin N. Y. Shavina M. V. Ovchinnikova T. Y. Kirilova L. F. Livanova N. I. Belyakova
author_sort	A. P. Semakova
title	Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
title_short	Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
title_full	Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
title_fullStr	Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
title_full_unstemmed	Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
title_sort	anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/e0f287de53464a7b9322d6851ef926da
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Anesthesia of laboratory animals in manufacturing of diagnostic and preventive biomedicines

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