A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

ABSTRACT N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system. We mapped transcription termination sites affected by AHL QS in P. aeruginosa, and in do...

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Autores principales:	Maureen K. Thomason, Maya Voichek, Daniel Dar, Victoria Addis, David Fitzgerald, Susan Gottesman, Rotem Sorek, E. Peter Greenberg
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	term-seq transcriptome small RNA Hfq pyoverdine Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e0f5909112bb4c20a576f27cc4337356
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spelling	oai:doaj.org-article:e0f5909112bb4c20a576f27cc43373562021-11-15T15:59:42ZA <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>10.1128/mBio.02253-192150-7511https://doaj.org/article/e0f5909112bb4c20a576f27cc43373562019-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02253-19https://doaj.org/toc/2150-7511ABSTRACT N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system. We mapped transcription termination sites affected by AHL QS in P. aeruginosa, and in doing so we identified AHL-regulated small RNAs (sRNAs). Of interest, we noted that one particular sRNA was located within the rhlI locus. We found that rhlI, which encodes the enzyme that produces the AHL N-butanoyl-homoserine lactone (C4-HSL), is controlled by a 5′ untranslated region (UTR)-derived sRNA we name RhlS. We also identified an antisense RNA encoded opposite the beginning of the rhlI open reading frame, which we name asRhlS. RhlS accumulates as wild-type cells enter stationary phase and is required for the production of normal levels of C4-HSL through activation of rhlI translation. RhlS also directly posttranscriptionally regulates at least one other unlinked gene, fpvA. The asRhlS appears to be expressed at maximal levels during logarithmic growth, and we suggest RhlS may act antagonistically to the asRhlS to regulate rhlI translation. The rhlI-encoded sRNAs represent a novel aspect of RNA-mediated tuning of P. aeruginosa QS. IMPORTANCE The opportunistic human pathogen Pseudomonas aeruginosa possesses multiple quorum sensing systems that regulate and coordinate production of virulence factors and adaptation to different environments. Despite extensive research, the regulatory elements that play a role in this complex network are still not fully understood. By using several RNA sequencing techniques, we were able to identify a small regulatory RNA we named RhlS. RhlS increases translation of RhlI, a key enzyme in the quorum sensing pathway, and represses the fpvA mRNA encoding one of the siderophore pyoverdine receptors. Our results highlight a new regulatory layer of P. aeruginosa quorum sensing and contribute to the growing understanding of the role regulatory RNAs play in bacterial physiology.Maureen K. ThomasonMaya VoichekDaniel DarVictoria AddisDavid FitzgeraldSusan GottesmanRotem SorekE. Peter GreenbergAmerican Society for Microbiologyarticleterm-seqtranscriptomesmall RNAHfqpyoverdineMicrobiologyQR1-502ENmBio, Vol 10, Iss 5 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	term-seq transcriptome small RNA Hfq pyoverdine Microbiology QR1-502
spellingShingle	term-seq transcriptome small RNA Hfq pyoverdine Microbiology QR1-502 Maureen K. Thomason Maya Voichek Daniel Dar Victoria Addis David Fitzgerald Susan Gottesman Rotem Sorek E. Peter Greenberg A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
description	ABSTRACT N-Acyl homoserine lactone (AHL) quorum sensing (QS) controls expression of over 200 genes in Pseudomonas aeruginosa. There are two AHL regulatory systems: the LasR-LasI circuit and the RhlR-RhlI system. We mapped transcription termination sites affected by AHL QS in P. aeruginosa, and in doing so we identified AHL-regulated small RNAs (sRNAs). Of interest, we noted that one particular sRNA was located within the rhlI locus. We found that rhlI, which encodes the enzyme that produces the AHL N-butanoyl-homoserine lactone (C4-HSL), is controlled by a 5′ untranslated region (UTR)-derived sRNA we name RhlS. We also identified an antisense RNA encoded opposite the beginning of the rhlI open reading frame, which we name asRhlS. RhlS accumulates as wild-type cells enter stationary phase and is required for the production of normal levels of C4-HSL through activation of rhlI translation. RhlS also directly posttranscriptionally regulates at least one other unlinked gene, fpvA. The asRhlS appears to be expressed at maximal levels during logarithmic growth, and we suggest RhlS may act antagonistically to the asRhlS to regulate rhlI translation. The rhlI-encoded sRNAs represent a novel aspect of RNA-mediated tuning of P. aeruginosa QS. IMPORTANCE The opportunistic human pathogen Pseudomonas aeruginosa possesses multiple quorum sensing systems that regulate and coordinate production of virulence factors and adaptation to different environments. Despite extensive research, the regulatory elements that play a role in this complex network are still not fully understood. By using several RNA sequencing techniques, we were able to identify a small regulatory RNA we named RhlS. RhlS increases translation of RhlI, a key enzyme in the quorum sensing pathway, and represses the fpvA mRNA encoding one of the siderophore pyoverdine receptors. Our results highlight a new regulatory layer of P. aeruginosa quorum sensing and contribute to the growing understanding of the role regulatory RNAs play in bacterial physiology.
format	article
author	Maureen K. Thomason Maya Voichek Daniel Dar Victoria Addis David Fitzgerald Susan Gottesman Rotem Sorek E. Peter Greenberg
author_facet	Maureen K. Thomason Maya Voichek Daniel Dar Victoria Addis David Fitzgerald Susan Gottesman Rotem Sorek E. Peter Greenberg
author_sort	Maureen K. Thomason
title	A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_short	A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full	A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_fullStr	A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_full_unstemmed	A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>
title_sort	<italic toggle="yes">rhli</italic> 5′ utr-derived srna regulates rhlr-dependent quorum sensing in <named-content content-type="genus-species">pseudomonas aeruginosa</named-content>
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/e0f5909112bb4c20a576f27cc4337356
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A <italic toggle="yes">rhlI</italic> 5′ UTR-Derived sRNA Regulates RhlR-Dependent Quorum Sensing in <named-content content-type="genus-species">Pseudomonas aeruginosa</named-content>

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