Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer

Abstract B7-H6 and PD-L1 belong to the B7 family co-stimulatory molecules fine-tuning the immune response. The present work investigates the clinical effect of B7-H6 protein expression with PD-L1 status and the infiltration of natural killer cells as potential biomarkers in breast tumor inflammatory...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Boutheina Cherif, Hana Triki, Slim Charfi, Lobna Bouzidi, Wala Ben Kridis, Afef Khanfir, Kais Chaabane, Tahya Sellami-Boudawara, Ahmed Rebai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0f66d5c1c884bce981019b3b9ea42ee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e0f66d5c1c884bce981019b3b9ea42ee
record_format	dspace
spelling	oai:doaj.org-article:e0f66d5c1c884bce981019b3b9ea42ee2021-12-02T18:15:24ZImmune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer10.1038/s41598-021-87216-92045-2322https://doaj.org/article/e0f66d5c1c884bce981019b3b9ea42ee2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87216-9https://doaj.org/toc/2045-2322Abstract B7-H6 and PD-L1 belong to the B7 family co-stimulatory molecules fine-tuning the immune response. The present work investigates the clinical effect of B7-H6 protein expression with PD-L1 status and the infiltration of natural killer cells as potential biomarkers in breast tumor inflammatory microenvironment. The expression levels of B7-H6 protein by cancer cells and immune infiltrating cells in human breast cancer tissues and evaluate their associations with PD-L1 expression, NK cell status, clinical pathological features and prognosis were explored. The immunohistochemistry labeling method was used to assess B7-H6 and PD-L1 proteins expression by cancer and immune cells. The associations between immune checkpoint, major clinical pathological variables and survival rates were analyzed. B7-H6 protein was depicted in both breast and immune cells. Results showed that Tumor B7-H6 expression is highly associated with Her-2 over expression. B7-H6 + immune cells are highly related to the Scarff–Bloom–Richardson grade and associated with PD-L1 expression and NK cells status. Survival analysis revealed a better prognosis in patients with low expression of B7-H6 by cancer cells. Conversely, B7-H6 + immune cells were significantly associated with longer survival. Findings strongly suggest an interaction between B7 molecules that contributes to a particular design of the inflammatory microenvironment. This may influence the efficiency of therapies based on antibodies blocking the PD-L1/PD1 pathway and can explain the detection of clinical benefits only in a fraction of patients treated with immune checkpoint inhibitors.Boutheina CherifHana TrikiSlim CharfiLobna BouzidiWala Ben KridisAfef KhanfirKais ChaabaneTahya Sellami-BoudawaraAhmed RebaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Boutheina Cherif Hana Triki Slim Charfi Lobna Bouzidi Wala Ben Kridis Afef Khanfir Kais Chaabane Tahya Sellami-Boudawara Ahmed Rebai Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
description	Abstract B7-H6 and PD-L1 belong to the B7 family co-stimulatory molecules fine-tuning the immune response. The present work investigates the clinical effect of B7-H6 protein expression with PD-L1 status and the infiltration of natural killer cells as potential biomarkers in breast tumor inflammatory microenvironment. The expression levels of B7-H6 protein by cancer cells and immune infiltrating cells in human breast cancer tissues and evaluate their associations with PD-L1 expression, NK cell status, clinical pathological features and prognosis were explored. The immunohistochemistry labeling method was used to assess B7-H6 and PD-L1 proteins expression by cancer and immune cells. The associations between immune checkpoint, major clinical pathological variables and survival rates were analyzed. B7-H6 protein was depicted in both breast and immune cells. Results showed that Tumor B7-H6 expression is highly associated with Her-2 over expression. B7-H6 + immune cells are highly related to the Scarff–Bloom–Richardson grade and associated with PD-L1 expression and NK cells status. Survival analysis revealed a better prognosis in patients with low expression of B7-H6 by cancer cells. Conversely, B7-H6 + immune cells were significantly associated with longer survival. Findings strongly suggest an interaction between B7 molecules that contributes to a particular design of the inflammatory microenvironment. This may influence the efficiency of therapies based on antibodies blocking the PD-L1/PD1 pathway and can explain the detection of clinical benefits only in a fraction of patients treated with immune checkpoint inhibitors.
format	article
author	Boutheina Cherif Hana Triki Slim Charfi Lobna Bouzidi Wala Ben Kridis Afef Khanfir Kais Chaabane Tahya Sellami-Boudawara Ahmed Rebai
author_facet	Boutheina Cherif Hana Triki Slim Charfi Lobna Bouzidi Wala Ben Kridis Afef Khanfir Kais Chaabane Tahya Sellami-Boudawara Ahmed Rebai
author_sort	Boutheina Cherif
title	Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
title_short	Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
title_full	Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
title_fullStr	Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
title_full_unstemmed	Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer
title_sort	immune checkpoint molecules b7-h6 and pd-l1 co-pattern the tumor inflammatory microenvironment in human breast cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e0f66d5c1c884bce981019b3b9ea42ee
work_keys_str_mv	AT boutheinacherif immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT hanatriki immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT slimcharfi immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT lobnabouzidi immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT walabenkridis immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT afefkhanfir immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT kaischaabane immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT tahyasellamiboudawara immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer AT ahmedrebai immunecheckpointmoleculesb7h6andpdl1copatternthetumorinflammatorymicroenvironmentinhumanbreastcancer
_version_	1718378426907230208

Immune checkpoint molecules B7-H6 and PD-L1 co-pattern the tumor inflammatory microenvironment in human breast cancer

Ejemplares similares