Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart

Abstract The expression of the channels-enzymes TRPM6 and TRPM7 in the human heart remains poorly defined, and TRPM6 is generally considered not to be expressed in cardiomyocytes. We examined their expression at protein and mRNA levels using right atrial samples resected from patients (n = 72) with...

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Autores principales:	Inga Andriulė, Dalia Pangonytė, Mantė Almanaitytė, Vaiva Patamsytė, Milda Kuprytė, Dainius Karčiauskas, Kanigula Mubagwa, Regina Mačianskienė
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0f6dda8283848018f01361c5601c662
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spelling	oai:doaj.org-article:e0f6dda8283848018f01361c5601c6622021-12-02T16:30:09ZEvidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart10.1038/s41598-021-94856-42045-2322https://doaj.org/article/e0f6dda8283848018f01361c5601c6622021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94856-4https://doaj.org/toc/2045-2322Abstract The expression of the channels-enzymes TRPM6 and TRPM7 in the human heart remains poorly defined, and TRPM6 is generally considered not to be expressed in cardiomyocytes. We examined their expression at protein and mRNA levels using right atrial samples resected from patients (n = 72) with or without ischemic heart disease (IHD) and samples from all chamber walls of explanted human hearts (n = 9). TRPM6 and TRPM7 proteins were detected using immunofluorescence on isolated cardiomyocytes, ELISA on tissue homogenates, and immunostaining of cardiac tissue, whereas their mRNAs were detected by RT-qPCR. Both TRPM6 and TRPM7 were present in all chamber walls, with TRPM7 being more abundant. TRPM6 was co-expressed with TRPM7. The expression levels were dependent on cell incubation conditions (presence or absence of divalent cations, pH of the extracellular milieu, presence of TRP channel inhibitors 2-aminoethoxydiphenyl-borate and carvacrol). These drugs reduced TRPM7 immunofluorescence but increased that of TRPM6. TRPM6 and TRPM7 expression was increased in tissues from IHD patients. This is the first demonstration of the presence and co-expression of TRPM6 and TRPM7 in cardiomyocytes from all chamber walls of the human heart. The increased TRPM6 and TRPM7 expression in IHD suggests that the chanzymes are involved in the pathophysiology of the disease.Inga AndriulėDalia PangonytėMantė AlmanaitytėVaiva PatamsytėMilda KuprytėDainius KarčiauskasKanigula MubagwaRegina MačianskienėNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Inga Andriulė Dalia Pangonytė Mantė Almanaitytė Vaiva Patamsytė Milda Kuprytė Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
description	Abstract The expression of the channels-enzymes TRPM6 and TRPM7 in the human heart remains poorly defined, and TRPM6 is generally considered not to be expressed in cardiomyocytes. We examined their expression at protein and mRNA levels using right atrial samples resected from patients (n = 72) with or without ischemic heart disease (IHD) and samples from all chamber walls of explanted human hearts (n = 9). TRPM6 and TRPM7 proteins were detected using immunofluorescence on isolated cardiomyocytes, ELISA on tissue homogenates, and immunostaining of cardiac tissue, whereas their mRNAs were detected by RT-qPCR. Both TRPM6 and TRPM7 were present in all chamber walls, with TRPM7 being more abundant. TRPM6 was co-expressed with TRPM7. The expression levels were dependent on cell incubation conditions (presence or absence of divalent cations, pH of the extracellular milieu, presence of TRP channel inhibitors 2-aminoethoxydiphenyl-borate and carvacrol). These drugs reduced TRPM7 immunofluorescence but increased that of TRPM6. TRPM6 and TRPM7 expression was increased in tissues from IHD patients. This is the first demonstration of the presence and co-expression of TRPM6 and TRPM7 in cardiomyocytes from all chamber walls of the human heart. The increased TRPM6 and TRPM7 expression in IHD suggests that the chanzymes are involved in the pathophysiology of the disease.
format	article
author	Inga Andriulė Dalia Pangonytė Mantė Almanaitytė Vaiva Patamsytė Milda Kuprytė Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė
author_facet	Inga Andriulė Dalia Pangonytė Mantė Almanaitytė Vaiva Patamsytė Milda Kuprytė Dainius Karčiauskas Kanigula Mubagwa Regina Mačianskienė
author_sort	Inga Andriulė
title	Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
title_short	Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
title_full	Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
title_fullStr	Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
title_full_unstemmed	Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart
title_sort	evidence for the expression of trpm6 and trpm7 in cardiomyocytes from all four chamber walls of the human heart
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e0f6dda8283848018f01361c5601c662
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Evidence for the expression of TRPM6 and TRPM7 in cardiomyocytes from all four chamber walls of the human heart

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