Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.

We previously reported that dye was effective to prevent the leakage of enzyme solutions from pancreatic glands during an islet isolation procedure. However, the dye used for islet isolation has not yet been optimized. In this study, we focused on pyoktanin blue (PB), diagnogreen (DG), and indigo ca...

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Autores principales:	Takehiro Imura, Akiko Inagaki, Yasuhiro Igarashi, Masafumi Goto
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e0f77dbd53c24acd9f26b44d8d75868b
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spelling	oai:doaj.org-article:e0f77dbd53c24acd9f26b44d8d75868b2021-12-02T20:19:37ZOptimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.1932-620310.1371/journal.pone.0255733https://doaj.org/article/e0f77dbd53c24acd9f26b44d8d75868b2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255733https://doaj.org/toc/1932-6203We previously reported that dye was effective to prevent the leakage of enzyme solutions from pancreatic glands during an islet isolation procedure. However, the dye used for islet isolation has not yet been optimized. In this study, we focused on pyoktanin blue (PB), diagnogreen (DG), and indigo carmine (IC) as potential candidates among clinically established dyes. A serial dilution assay was performed to determine minimal effective concentrations of each dye for detecting damaged pancreatic tissues. According to the outcome of serial dilution assays, double minimum effective concentrations of each dye were used for in vitro toxicity assays on islets and used in the isolation procedure to investigate whether they adversely affect islet isolation efficiency. The evaluations included islet yield, ADP/ATP, ATP/DNA, glucose stimulation test, and insulin/DNA assays. Islet viability cultured with PB contained medium was significantly lower than the other dyes. DG and IC appeared to be non-toxic to the islets. In isolation experiments, the islet yield in the DG group was considerably lower than that in the Control group, suggesting that DG might inhibit enzyme activity. The present study demonstrates that IC could be a promising candidate for an effective dye to detect damaged pancreatic tissues without affecting the enzyme activity and islet quality.Takehiro ImuraAkiko InagakiYasuhiro IgarashiMasafumi GotoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255733 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takehiro Imura Akiko Inagaki Yasuhiro Igarashi Masafumi Goto Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
description	We previously reported that dye was effective to prevent the leakage of enzyme solutions from pancreatic glands during an islet isolation procedure. However, the dye used for islet isolation has not yet been optimized. In this study, we focused on pyoktanin blue (PB), diagnogreen (DG), and indigo carmine (IC) as potential candidates among clinically established dyes. A serial dilution assay was performed to determine minimal effective concentrations of each dye for detecting damaged pancreatic tissues. According to the outcome of serial dilution assays, double minimum effective concentrations of each dye were used for in vitro toxicity assays on islets and used in the isolation procedure to investigate whether they adversely affect islet isolation efficiency. The evaluations included islet yield, ADP/ATP, ATP/DNA, glucose stimulation test, and insulin/DNA assays. Islet viability cultured with PB contained medium was significantly lower than the other dyes. DG and IC appeared to be non-toxic to the islets. In isolation experiments, the islet yield in the DG group was considerably lower than that in the Control group, suggesting that DG might inhibit enzyme activity. The present study demonstrates that IC could be a promising candidate for an effective dye to detect damaged pancreatic tissues without affecting the enzyme activity and islet quality.
format	article
author	Takehiro Imura Akiko Inagaki Yasuhiro Igarashi Masafumi Goto
author_facet	Takehiro Imura Akiko Inagaki Yasuhiro Igarashi Masafumi Goto
author_sort	Takehiro Imura
title	Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
title_short	Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
title_full	Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
title_fullStr	Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
title_full_unstemmed	Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
title_sort	optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e0f77dbd53c24acd9f26b44d8d75868b
work_keys_str_mv	AT takehiroimura optimizationofdyesolutionsfordetectingdamagedpancreatictissuesduringisletisolationprocedures AT akikoinagaki optimizationofdyesolutionsfordetectingdamagedpancreatictissuesduringisletisolationprocedures AT yasuhiroigarashi optimizationofdyesolutionsfordetectingdamagedpancreatictissuesduringisletisolationprocedures AT masafumigoto optimizationofdyesolutionsfordetectingdamagedpancreatictissuesduringisletisolationprocedures
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Optimization of dye solutions for detecting damaged pancreatic tissues during islet isolation procedures.

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