The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.

Photorhabdus is a genus of Gram-negative entomopathogenic bacteria that also maintain a mutualistic association with nematodes from the family Heterorhabditis. Photorhabdus has an extensive secondary metabolism that is required for the interaction between the bacteria and the nematode. A major compo...

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Autores principales:	Lea Lango-Scholey, Alexander O Brachmann, Helge B Bode, David J Clarke
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1010306aa9d48f7a1ccb8736118b704
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spelling	oai:doaj.org-article:e1010306aa9d48f7a1ccb8736118b7042021-11-18T08:44:57ZThe expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.1932-620310.1371/journal.pone.0082152https://doaj.org/article/e1010306aa9d48f7a1ccb8736118b7042013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278476/?tool=EBIhttps://doaj.org/toc/1932-6203Photorhabdus is a genus of Gram-negative entomopathogenic bacteria that also maintain a mutualistic association with nematodes from the family Heterorhabditis. Photorhabdus has an extensive secondary metabolism that is required for the interaction between the bacteria and the nematode. A major component of this secondary metabolism is a stilbene molecule, called ST. The first step in ST biosynthesis is the non-oxidative deamination of phenylalanine resulting in the production of cinnamic acid. This reaction is catalyzed by phenylalanine-ammonium lyase, an enzyme encoded by the stlA gene. In this study we show, using a stlA-gfp transcriptional fusion, that the expression of stlA is regulated by nutrient limitation through a regulatory network that involves at least 3 regulators. We show that TyrR, a LysR-type transcriptional regulator that regulates gene expression in response to aromatic amino acids in E. coli, is absolutely required for stlA expression. We also show that stlA expression is modulated by σ(S) and Lrp, regulators that are implicated in the regulation of the response to nutrient limitation in other bacteria. This work is the first that describes pathway-specific regulation of secondary metabolism in Photorhabdus and, therefore, our study provides an initial insight into the complex regulatory network that controls secondary metabolism, and therefore mutualism, in this model organism.Lea Lango-ScholeyAlexander O BrachmannHelge B BodeDavid J ClarkePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e82152 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lea Lango-Scholey Alexander O Brachmann Helge B Bode David J Clarke The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.
description	Photorhabdus is a genus of Gram-negative entomopathogenic bacteria that also maintain a mutualistic association with nematodes from the family Heterorhabditis. Photorhabdus has an extensive secondary metabolism that is required for the interaction between the bacteria and the nematode. A major component of this secondary metabolism is a stilbene molecule, called ST. The first step in ST biosynthesis is the non-oxidative deamination of phenylalanine resulting in the production of cinnamic acid. This reaction is catalyzed by phenylalanine-ammonium lyase, an enzyme encoded by the stlA gene. In this study we show, using a stlA-gfp transcriptional fusion, that the expression of stlA is regulated by nutrient limitation through a regulatory network that involves at least 3 regulators. We show that TyrR, a LysR-type transcriptional regulator that regulates gene expression in response to aromatic amino acids in E. coli, is absolutely required for stlA expression. We also show that stlA expression is modulated by σ(S) and Lrp, regulators that are implicated in the regulation of the response to nutrient limitation in other bacteria. This work is the first that describes pathway-specific regulation of secondary metabolism in Photorhabdus and, therefore, our study provides an initial insight into the complex regulatory network that controls secondary metabolism, and therefore mutualism, in this model organism.
format	article
author	Lea Lango-Scholey Alexander O Brachmann Helge B Bode David J Clarke
author_facet	Lea Lango-Scholey Alexander O Brachmann Helge B Bode David J Clarke
author_sort	Lea Lango-Scholey
title	The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.
title_short	The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.
title_full	The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.
title_fullStr	The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.
title_full_unstemmed	The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.
title_sort	expression of stla in photorhabdus luminescens is controlled by nutrient limitation.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e1010306aa9d48f7a1ccb8736118b704
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The expression of stlA in Photorhabdus luminescens is controlled by nutrient limitation.

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