A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing.
Although long noncoding RNAs (lncRNAs) are transcripts that do not encode proteins by definition, some lncRNAs actually contain small open reading frames that are translated. TINCR (terminal differentiation-induced ncRNA) has been recognized as a lncRNA that contributes to keratinocyte differentiati...
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2021
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oai:doaj.org-article:e117df6c413b4eef8868600148f26c7b2021-12-02T20:03:24ZA ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing.1553-73901553-740410.1371/journal.pgen.1009686https://doaj.org/article/e117df6c413b4eef8868600148f26c7b2021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009686https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Although long noncoding RNAs (lncRNAs) are transcripts that do not encode proteins by definition, some lncRNAs actually contain small open reading frames that are translated. TINCR (terminal differentiation-induced ncRNA) has been recognized as a lncRNA that contributes to keratinocyte differentiation. However, we here show that TINCR encodes a ubiquitin-like protein that is well conserved among species and whose expression was confirmed by the generation of mice harboring a FLAG epitope tag sequence in the endogenous open reading frame as well as by targeted proteomics. Forced expression of this protein promoted cell cycle progression in normal human epidermal keratinocytes, and mice lacking this protein manifested a delay in skin wound healing associated with attenuated cell cycle progression in keratinocytes. We termed this protein TINCR-encoded ubiquitin-like protein (TUBL), and our results reveal a role for TINCR in the regulation of keratinocyte proliferation and skin regeneration that is dependent on TUBL.Akihiro NitaAkinobu MatsumotoRonghao TangChisa ShiraishiKazuya IchiharaDaisuke SaitoMikita SuyamaTomoharu YasudaGaku TsujiMasutaka FurueBumpei KatayamaToshiyuki OzawaTeruasa MurataTeruki DainichiKenji KabashimaAtsushi HatanoMasaki MatsumotoKeiichi I NakayamaPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009686 (2021) |
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DOAJ |
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Genetics QH426-470 |
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Genetics QH426-470 Akihiro Nita Akinobu Matsumoto Ronghao Tang Chisa Shiraishi Kazuya Ichihara Daisuke Saito Mikita Suyama Tomoharu Yasuda Gaku Tsuji Masutaka Furue Bumpei Katayama Toshiyuki Ozawa Teruasa Murata Teruki Dainichi Kenji Kabashima Atsushi Hatano Masaki Matsumoto Keiichi I Nakayama A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing. |
| description |
Although long noncoding RNAs (lncRNAs) are transcripts that do not encode proteins by definition, some lncRNAs actually contain small open reading frames that are translated. TINCR (terminal differentiation-induced ncRNA) has been recognized as a lncRNA that contributes to keratinocyte differentiation. However, we here show that TINCR encodes a ubiquitin-like protein that is well conserved among species and whose expression was confirmed by the generation of mice harboring a FLAG epitope tag sequence in the endogenous open reading frame as well as by targeted proteomics. Forced expression of this protein promoted cell cycle progression in normal human epidermal keratinocytes, and mice lacking this protein manifested a delay in skin wound healing associated with attenuated cell cycle progression in keratinocytes. We termed this protein TINCR-encoded ubiquitin-like protein (TUBL), and our results reveal a role for TINCR in the regulation of keratinocyte proliferation and skin regeneration that is dependent on TUBL. |
| format |
article |
| author |
Akihiro Nita Akinobu Matsumoto Ronghao Tang Chisa Shiraishi Kazuya Ichihara Daisuke Saito Mikita Suyama Tomoharu Yasuda Gaku Tsuji Masutaka Furue Bumpei Katayama Toshiyuki Ozawa Teruasa Murata Teruki Dainichi Kenji Kabashima Atsushi Hatano Masaki Matsumoto Keiichi I Nakayama |
| author_facet |
Akihiro Nita Akinobu Matsumoto Ronghao Tang Chisa Shiraishi Kazuya Ichihara Daisuke Saito Mikita Suyama Tomoharu Yasuda Gaku Tsuji Masutaka Furue Bumpei Katayama Toshiyuki Ozawa Teruasa Murata Teruki Dainichi Kenji Kabashima Atsushi Hatano Masaki Matsumoto Keiichi I Nakayama |
| author_sort |
Akihiro Nita |
| title |
A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing. |
| title_short |
A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing. |
| title_full |
A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing. |
| title_fullStr |
A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing. |
| title_full_unstemmed |
A ubiquitin-like protein encoded by the "noncoding" RNA TINCR promotes keratinocyte proliferation and wound healing. |
| title_sort |
ubiquitin-like protein encoded by the "noncoding" rna tincr promotes keratinocyte proliferation and wound healing. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/e117df6c413b4eef8868600148f26c7b |
| work_keys_str_mv |
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1718375692892110848 |