Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun
Abstract In the majority of sexual eukaryotes, the mitochondrial genomes are inherited uniparentally. As a result, individual organisms are homoplasmic, containing mitochondrial DNA (mtDNA) from a single parent. Here we analyzed the mitochondrial genotypes in Clade I of the gourmet mushroom Thelepho...
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oai:doaj.org-article:e119defc073f433d92716972691c89702021-12-02T16:06:45ZFrequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun10.1038/s41598-017-01823-z2045-2322https://doaj.org/article/e119defc073f433d92716972691c89702017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01823-zhttps://doaj.org/toc/2045-2322Abstract In the majority of sexual eukaryotes, the mitochondrial genomes are inherited uniparentally. As a result, individual organisms are homoplasmic, containing mitochondrial DNA (mtDNA) from a single parent. Here we analyzed the mitochondrial genotypes in Clade I of the gourmet mushroom Thelephora ganbajun from its broad geographic distribution range. A total of 299 isolates from 28 geographic locations were sequenced at three mitochondrial loci: the mitochondrial small ribosomal RNA gene, and the cytochrome c oxidase subunits I (COX1) and III (COX3) genes. Quantitative PCR analyses showed that the strains had about 60–160 copies of mitochondrial genomes per cell. Interestingly, while no evidence of heteroplasmy was found at the 12S rRNA gene, 262 of the 299 isolates had clear evidence of heterogeneity at either the COX1 (261 isolates) or COX3 (12 isolates) gene fragments. The COX1 heteroplasmy was characterized by two types of introns residing at different sites of the same region and at different frequencies among the isolates. Allelic association analyses of the observed mitochondrial polymorphic nucleotide sites suggest that mtDNA recombination is common in natural populations of this fungus. Our results contrast the prevailing view that heteroplasmy, if exists, is only transient in basidiomycete fungi.Pengfei WangTao ShaYunrun ZhangYang CaoFei MiCunli LiuDan YangXiaozhao TangXiaoxia HeJianyong DongJinyan WuShanze YoellLiam YoellKe-Qin ZhangYing ZhangJianping XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017) |
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Medicine R Science Q Pengfei Wang Tao Sha Yunrun Zhang Yang Cao Fei Mi Cunli Liu Dan Yang Xiaozhao Tang Xiaoxia He Jianyong Dong Jinyan Wu Shanze Yoell Liam Yoell Ke-Qin Zhang Ying Zhang Jianping Xu Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun |
| description |
Abstract In the majority of sexual eukaryotes, the mitochondrial genomes are inherited uniparentally. As a result, individual organisms are homoplasmic, containing mitochondrial DNA (mtDNA) from a single parent. Here we analyzed the mitochondrial genotypes in Clade I of the gourmet mushroom Thelephora ganbajun from its broad geographic distribution range. A total of 299 isolates from 28 geographic locations were sequenced at three mitochondrial loci: the mitochondrial small ribosomal RNA gene, and the cytochrome c oxidase subunits I (COX1) and III (COX3) genes. Quantitative PCR analyses showed that the strains had about 60–160 copies of mitochondrial genomes per cell. Interestingly, while no evidence of heteroplasmy was found at the 12S rRNA gene, 262 of the 299 isolates had clear evidence of heterogeneity at either the COX1 (261 isolates) or COX3 (12 isolates) gene fragments. The COX1 heteroplasmy was characterized by two types of introns residing at different sites of the same region and at different frequencies among the isolates. Allelic association analyses of the observed mitochondrial polymorphic nucleotide sites suggest that mtDNA recombination is common in natural populations of this fungus. Our results contrast the prevailing view that heteroplasmy, if exists, is only transient in basidiomycete fungi. |
| format |
article |
| author |
Pengfei Wang Tao Sha Yunrun Zhang Yang Cao Fei Mi Cunli Liu Dan Yang Xiaozhao Tang Xiaoxia He Jianyong Dong Jinyan Wu Shanze Yoell Liam Yoell Ke-Qin Zhang Ying Zhang Jianping Xu |
| author_facet |
Pengfei Wang Tao Sha Yunrun Zhang Yang Cao Fei Mi Cunli Liu Dan Yang Xiaozhao Tang Xiaoxia He Jianyong Dong Jinyan Wu Shanze Yoell Liam Yoell Ke-Qin Zhang Ying Zhang Jianping Xu |
| author_sort |
Pengfei Wang |
| title |
Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun |
| title_short |
Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun |
| title_full |
Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun |
| title_fullStr |
Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun |
| title_full_unstemmed |
Frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom Thelephora ganbajun |
| title_sort |
frequent heteroplasmy and recombination in the mitochondrial genomes of the basidiomycete mushroom thelephora ganbajun |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/e119defc073f433d92716972691c8970 |
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