The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal

Abstract Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However,...

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Autores principales:	Adriana Valeria Jäger, Paula Arias, Maria Virginia Tribulatti, Marcela Adriana Brocco, Maria Victoria Pepe, Arlinet Kierbel
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e11af27c9800490ca7812f0cb33315ab
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spelling	oai:doaj.org-article:e11af27c9800490ca7812f0cb33315ab2021-12-02T14:16:07ZThe inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal10.1038/s41598-021-81557-12045-2322https://doaj.org/article/e11af27c9800490ca7812f0cb33315ab2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81557-1https://doaj.org/toc/2045-2322Abstract Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions have shown to be more complex. Furthermore, little is known about the regulation of efferocytosis under inflammatory conditions. In this study, we elucidate the modulation of the macrophage efferocytic function during an inflammatory stimulus. We find that bone marrow-derived macrophages (BMDM) are very efficient in engulfing both the bacterial pathogen Pseudomonas aeruginosa and apoptotic cells. BMDM showed a high bactericidal capacity unaffected by the concomitant presence of apoptotic material. Plasticity in macrophage programming, in response to changing environmental cues, may modulate efferocytic capability. In this work, we further show that, after phagocyting and processing Pseudomonas aeruginosa, macrophages highly increase their efferocytic capacity without affecting their phagocytic function. Moreover, we demonstrate that Pseudomonas aeruginosa enhances efferocytosis of these phagocytes through the IL-6 signaling pathway. Our results show that the inflammatory response generated by the bacterial processing enhances these macrophages’ capacity to control inflammation through an increased efferocytosis.Adriana Valeria JägerPaula AriasMaria Virginia TribulattiMarcela Adriana BroccoMaria Victoria PepeArlinet KierbelNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adriana Valeria Jäger Paula Arias Maria Virginia Tribulatti Marcela Adriana Brocco Maria Victoria Pepe Arlinet Kierbel The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
description	Abstract Pathogens phagocytosis and the uptake of apoptotic cells (efferocytosis) are essential macrophages tasks, classically considered as mutually exclusive. Macrophages have been observed to polarize into either pro-inflammatory/microbicidal or anti-inflammatory/efferocytic phenotypes. However, macrophage functions have shown to be more complex. Furthermore, little is known about the regulation of efferocytosis under inflammatory conditions. In this study, we elucidate the modulation of the macrophage efferocytic function during an inflammatory stimulus. We find that bone marrow-derived macrophages (BMDM) are very efficient in engulfing both the bacterial pathogen Pseudomonas aeruginosa and apoptotic cells. BMDM showed a high bactericidal capacity unaffected by the concomitant presence of apoptotic material. Plasticity in macrophage programming, in response to changing environmental cues, may modulate efferocytic capability. In this work, we further show that, after phagocyting and processing Pseudomonas aeruginosa, macrophages highly increase their efferocytic capacity without affecting their phagocytic function. Moreover, we demonstrate that Pseudomonas aeruginosa enhances efferocytosis of these phagocytes through the IL-6 signaling pathway. Our results show that the inflammatory response generated by the bacterial processing enhances these macrophages’ capacity to control inflammation through an increased efferocytosis.
format	article
author	Adriana Valeria Jäger Paula Arias Maria Virginia Tribulatti Marcela Adriana Brocco Maria Victoria Pepe Arlinet Kierbel
author_facet	Adriana Valeria Jäger Paula Arias Maria Virginia Tribulatti Marcela Adriana Brocco Maria Victoria Pepe Arlinet Kierbel
author_sort	Adriana Valeria Jäger
title	The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
title_short	The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
title_full	The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
title_fullStr	The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
title_full_unstemmed	The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
title_sort	inflammatory response induced by pseudomonas aeruginosa in macrophages enhances apoptotic cell removal
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e11af27c9800490ca7812f0cb33315ab
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The inflammatory response induced by Pseudomonas aeruginosa in macrophages enhances apoptotic cell removal

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