Tumor versus stromal cells in culture--survival of the fittest?

Two of the signature genetic events that occur in human gliomas, EGFR amplification and IDH mutation, are poorly represented in experimental models in vitro. EGFR amplification, for example, occurs in 40 to 50% of GBM, and yet, EGFR amplification is rarely preserved in cell cultures derived from hum...

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Autores principales:	Krishna M Talasila, Narve Brekka, Kjersti Mangseth, Daniel Stieber, Lasse Evensen, Gro V Rosland, Anja Torsvik, Marek Wagner, Simone P Niclou, Rupavathana Mahesparan, Olav K Vintermyr, Rolf Bjerkvig, Janice M Nigro, Hrvoje Miletic
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e11b2a7f9c3149ffb7dc882e61c265ab
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spelling	oai:doaj.org-article:e11b2a7f9c3149ffb7dc882e61c265ab2021-11-18T08:43:50ZTumor versus stromal cells in culture--survival of the fittest?1932-620310.1371/journal.pone.0081183https://doaj.org/article/e11b2a7f9c3149ffb7dc882e61c265ab2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24349039/?tool=EBIhttps://doaj.org/toc/1932-6203Two of the signature genetic events that occur in human gliomas, EGFR amplification and IDH mutation, are poorly represented in experimental models in vitro. EGFR amplification, for example, occurs in 40 to 50% of GBM, and yet, EGFR amplification is rarely preserved in cell cultures derived from human tumors. To analyze the fate of EGFR amplified and IDH mutated cells in culture, we followed the development over time of cultures derived from human xenografts in nude rats enriched for tumor cells with EGFR amplification and of cultures derived from patient samples with IDH mutations, in serum monolayer and spheroid suspension culture, under serum and serum free conditions. We observed under serum monolayer conditions, that nestin positive or nestin and SMA double positive rat stromal cells outgrew EGFR amplified tumor cells, while serum spheroid cultures preserved tumor cells with EGFR amplification. Serum free suspension culture exhibited a more variable cell composition in that the resultant cell populations were either predominantly nestin/SOX2 co-expressing rat stromal cells or human tumor cells, or a mixture of both. The selection for nestin/SMA positive stromal cells under serum monolayer conditions was also consistently observed in human oligodendrogliomas and oligoastrocytomas with IDH mutations. Our results highlight for the first time that serum monolayer conditions can select for stromal cells instead of tumor cells in certain brain tumor subtypes. This result has an important impact on the establishment of new tumor cell cultures from brain tumors and raises the question of the proper conditions for the growth of the tumor cell populations of interest.Krishna M TalasilaNarve BrekkaKjersti MangsethDaniel StieberLasse EvensenGro V RoslandAnja TorsvikMarek WagnerSimone P NiclouRupavathana MahesparanOlav K VintermyrRolf BjerkvigJanice M NigroHrvoje MileticPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81183 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Krishna M Talasila Narve Brekka Kjersti Mangseth Daniel Stieber Lasse Evensen Gro V Rosland Anja Torsvik Marek Wagner Simone P Niclou Rupavathana Mahesparan Olav K Vintermyr Rolf Bjerkvig Janice M Nigro Hrvoje Miletic Tumor versus stromal cells in culture--survival of the fittest?
description	Two of the signature genetic events that occur in human gliomas, EGFR amplification and IDH mutation, are poorly represented in experimental models in vitro. EGFR amplification, for example, occurs in 40 to 50% of GBM, and yet, EGFR amplification is rarely preserved in cell cultures derived from human tumors. To analyze the fate of EGFR amplified and IDH mutated cells in culture, we followed the development over time of cultures derived from human xenografts in nude rats enriched for tumor cells with EGFR amplification and of cultures derived from patient samples with IDH mutations, in serum monolayer and spheroid suspension culture, under serum and serum free conditions. We observed under serum monolayer conditions, that nestin positive or nestin and SMA double positive rat stromal cells outgrew EGFR amplified tumor cells, while serum spheroid cultures preserved tumor cells with EGFR amplification. Serum free suspension culture exhibited a more variable cell composition in that the resultant cell populations were either predominantly nestin/SOX2 co-expressing rat stromal cells or human tumor cells, or a mixture of both. The selection for nestin/SMA positive stromal cells under serum monolayer conditions was also consistently observed in human oligodendrogliomas and oligoastrocytomas with IDH mutations. Our results highlight for the first time that serum monolayer conditions can select for stromal cells instead of tumor cells in certain brain tumor subtypes. This result has an important impact on the establishment of new tumor cell cultures from brain tumors and raises the question of the proper conditions for the growth of the tumor cell populations of interest.
format	article
author	Krishna M Talasila Narve Brekka Kjersti Mangseth Daniel Stieber Lasse Evensen Gro V Rosland Anja Torsvik Marek Wagner Simone P Niclou Rupavathana Mahesparan Olav K Vintermyr Rolf Bjerkvig Janice M Nigro Hrvoje Miletic
author_facet	Krishna M Talasila Narve Brekka Kjersti Mangseth Daniel Stieber Lasse Evensen Gro V Rosland Anja Torsvik Marek Wagner Simone P Niclou Rupavathana Mahesparan Olav K Vintermyr Rolf Bjerkvig Janice M Nigro Hrvoje Miletic
author_sort	Krishna M Talasila
title	Tumor versus stromal cells in culture--survival of the fittest?
title_short	Tumor versus stromal cells in culture--survival of the fittest?
title_full	Tumor versus stromal cells in culture--survival of the fittest?
title_fullStr	Tumor versus stromal cells in culture--survival of the fittest?
title_full_unstemmed	Tumor versus stromal cells in culture--survival of the fittest?
title_sort	tumor versus stromal cells in culture--survival of the fittest?
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e11b2a7f9c3149ffb7dc882e61c265ab
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Tumor versus stromal cells in culture--survival of the fittest?

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