Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells

Abstract Successful implantation is dependent on the appropriate decidualization of endometrial stromal cells for the establishment of pregnancy in women. Mycobacterial heat shock protein 65 (HSP65) is involved in pathogenesis of the genital tuberculosis (GTB), one of the common causes of infertilit...

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Autores principales: Elavarasan Subramani, Arun Prabhu Rameshbabu, Manivannan Jothiramajayam, Bhuvaneshwaran Subramanian, Debangana Chakravorty, Gunja Bose, Mamata Joshi, Chaitali Datta Ray, Indrani Lodh, Ratna Chattopadhyay, Sudipto Saha, Anita Mukherjee, Santanu Dhara, Baidyanath Chakravarty, Koel Chaudhury
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:e11daf4492aa4b20b9224ce20af08f872021-12-02T16:06:59ZMycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells10.1038/s41598-017-04024-w2045-2322https://doaj.org/article/e11daf4492aa4b20b9224ce20af08f872017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04024-whttps://doaj.org/toc/2045-2322Abstract Successful implantation is dependent on the appropriate decidualization of endometrial stromal cells for the establishment of pregnancy in women. Mycobacterial heat shock protein 65 (HSP65) is involved in pathogenesis of the genital tuberculosis (GTB), one of the common causes of infertility in emerging countries. Though implantation failure appears to be the major cause, understanding the status of decidualizaiton process in women diagnosed with GTB has not been thoroughly addressed. We, therefore, explored the effect of HSP65 protein on the endometrial cell metabolism during in vitro decidualization. In order to identify the cellular metabolism of decidual cells with and without HSP65 treatment, proton NMR based characterization of metabolites extracted from cells and culture media were performed. In presence of HSP65, significant reduction in the decidual phenotype of endometrial stromal cells and prolactin expression is suggestive of impairment in decidualization. The intracellular and extracellular metabolic changes in HSP65 treated endometrial stromal cells produced a distinct pattern, reflecting the interaction between the protein and cellular metabolism. HSP65 mediated dysregulation in cellular metabolism is associated with poor decidualization. Besides enriching the present knowledge on metabolic changes underlying stromal cells decidualization, these findings assist in identifying potential molecular causes for decidualization failure in GTB women.Elavarasan SubramaniArun Prabhu RameshbabuManivannan JothiramajayamBhuvaneshwaran SubramanianDebangana ChakravortyGunja BoseMamata JoshiChaitali Datta RayIndrani LodhRatna ChattopadhyaySudipto SahaAnita MukherjeeSantanu DharaBaidyanath ChakravartyKoel ChaudhuryNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Elavarasan Subramani
Arun Prabhu Rameshbabu
Manivannan Jothiramajayam
Bhuvaneshwaran Subramanian
Debangana Chakravorty
Gunja Bose
Mamata Joshi
Chaitali Datta Ray
Indrani Lodh
Ratna Chattopadhyay
Sudipto Saha
Anita Mukherjee
Santanu Dhara
Baidyanath Chakravarty
Koel Chaudhury
Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
description Abstract Successful implantation is dependent on the appropriate decidualization of endometrial stromal cells for the establishment of pregnancy in women. Mycobacterial heat shock protein 65 (HSP65) is involved in pathogenesis of the genital tuberculosis (GTB), one of the common causes of infertility in emerging countries. Though implantation failure appears to be the major cause, understanding the status of decidualizaiton process in women diagnosed with GTB has not been thoroughly addressed. We, therefore, explored the effect of HSP65 protein on the endometrial cell metabolism during in vitro decidualization. In order to identify the cellular metabolism of decidual cells with and without HSP65 treatment, proton NMR based characterization of metabolites extracted from cells and culture media were performed. In presence of HSP65, significant reduction in the decidual phenotype of endometrial stromal cells and prolactin expression is suggestive of impairment in decidualization. The intracellular and extracellular metabolic changes in HSP65 treated endometrial stromal cells produced a distinct pattern, reflecting the interaction between the protein and cellular metabolism. HSP65 mediated dysregulation in cellular metabolism is associated with poor decidualization. Besides enriching the present knowledge on metabolic changes underlying stromal cells decidualization, these findings assist in identifying potential molecular causes for decidualization failure in GTB women.
format article
author Elavarasan Subramani
Arun Prabhu Rameshbabu
Manivannan Jothiramajayam
Bhuvaneshwaran Subramanian
Debangana Chakravorty
Gunja Bose
Mamata Joshi
Chaitali Datta Ray
Indrani Lodh
Ratna Chattopadhyay
Sudipto Saha
Anita Mukherjee
Santanu Dhara
Baidyanath Chakravarty
Koel Chaudhury
author_facet Elavarasan Subramani
Arun Prabhu Rameshbabu
Manivannan Jothiramajayam
Bhuvaneshwaran Subramanian
Debangana Chakravorty
Gunja Bose
Mamata Joshi
Chaitali Datta Ray
Indrani Lodh
Ratna Chattopadhyay
Sudipto Saha
Anita Mukherjee
Santanu Dhara
Baidyanath Chakravarty
Koel Chaudhury
author_sort Elavarasan Subramani
title Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
title_short Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
title_full Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
title_fullStr Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
title_full_unstemmed Mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
title_sort mycobacterial heat shock protein 65 mediated metabolic shift in decidualization of human endometrial stromal cells
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/e11daf4492aa4b20b9224ce20af08f87
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