Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers

Abstract. Kasiamdari RS, Aristya GR, Prabowo H, Ariesta C, Arif MF. 2019. Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers. Biodiversitas 20: 3005-3010. Sugarcane (Saccharum officinarum L.) belongs to the Poaceae family which are widely us...

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Autores principales:	RINA SRI KASIAMDARI, Ganies Riza Aristya, HERI PRABOWO, MUHAMMAD FAUZI ARIF, Christy Ariesta
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	clustering, genetic diversity, polymorphism, rapd, saccharum officinarum Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e1248736af6b49e7996ca2db0748386c
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spelling	oai:doaj.org-article:e1248736af6b49e7996ca2db0748386c2021-11-21T21:54:58ZShort Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers1412-033X2085-472210.13057/biodiv/d201032https://doaj.org/article/e1248736af6b49e7996ca2db0748386c2019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4073https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Kasiamdari RS, Aristya GR, Prabowo H, Ariesta C, Arif MF. 2019. Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers. Biodiversitas 20: 3005-3010. Sugarcane (Saccharum officinarum L.) belongs to the Poaceae family which are widely used for sweeteners production. In Indonesia, the need for sugarcane continues to increase along with its increased use in the production of bioethanol from molasses, a by-product of sugar production. However, the supply for sugar is still far from enough. Improvement of sugarcane productivity can be done by selecting superior varieties to form a new superior hybrid. Maintaining high genetic diversity in a population is essential. Selection of varieties is important because it can increase productivity without increasing the cost of crop production. Even the selected pest-resistant varieties can reduce the cost of crop production. Indonesia had produced many superior sugarcane plant varieties, but studies on their characters and genetic relationship are still lacking. Several molecular markers have been used to elucidate the genetic diversity among sugarcane varieties. Random Amplified Polymorphism DNA (RAPD) is one of the molecular markers that have been proven to describe genetic diversity among sugarcane plants. This study aimed to determine the genetic diversity among 22 superior sugarcane varieties in Indonesia based on eight RAPD molecular markers. The superior sugarcane varieties in Indonesia in this study were obtained from Indonesian Sweetener and Fiber Crops Research Institute (ISFRI), Malang and Madukismo Sugar Factory, Yogyakarta. DNA products were visualized by using gel electrophoresis and resolved bands were scored as binary data as presence (1) and absence (0). Linkage distance was done based on Jaccard’s similarity coefficient, and clustering analysis was performed based on the unweighted pair- group average (UPGMA) method. Polymorphism Information Content (PIC) analysis showed a high degree of polymorphism ranging from 0.76 to 0.92. Cluster analysis showed that 22 sugarcane varieties were divided into two main clusters and four sub-clusters based on their origin.RINA SRI KASIAMDARIGanies Riza AristyaHERI PRABOWOMUHAMMAD FAUZI ARIFChristy AriestaMBI & UNS Soloarticleclustering, genetic diversity, polymorphism, rapd, saccharum officinarumBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	clustering, genetic diversity, polymorphism, rapd, saccharum officinarum Biology (General) QH301-705.5
spellingShingle	clustering, genetic diversity, polymorphism, rapd, saccharum officinarum Biology (General) QH301-705.5 RINA SRI KASIAMDARI Ganies Riza Aristya HERI PRABOWO MUHAMMAD FAUZI ARIF Christy Ariesta Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers
description	Abstract. Kasiamdari RS, Aristya GR, Prabowo H, Ariesta C, Arif MF. 2019. Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers. Biodiversitas 20: 3005-3010. Sugarcane (Saccharum officinarum L.) belongs to the Poaceae family which are widely used for sweeteners production. In Indonesia, the need for sugarcane continues to increase along with its increased use in the production of bioethanol from molasses, a by-product of sugar production. However, the supply for sugar is still far from enough. Improvement of sugarcane productivity can be done by selecting superior varieties to form a new superior hybrid. Maintaining high genetic diversity in a population is essential. Selection of varieties is important because it can increase productivity without increasing the cost of crop production. Even the selected pest-resistant varieties can reduce the cost of crop production. Indonesia had produced many superior sugarcane plant varieties, but studies on their characters and genetic relationship are still lacking. Several molecular markers have been used to elucidate the genetic diversity among sugarcane varieties. Random Amplified Polymorphism DNA (RAPD) is one of the molecular markers that have been proven to describe genetic diversity among sugarcane plants. This study aimed to determine the genetic diversity among 22 superior sugarcane varieties in Indonesia based on eight RAPD molecular markers. The superior sugarcane varieties in Indonesia in this study were obtained from Indonesian Sweetener and Fiber Crops Research Institute (ISFRI), Malang and Madukismo Sugar Factory, Yogyakarta. DNA products were visualized by using gel electrophoresis and resolved bands were scored as binary data as presence (1) and absence (0). Linkage distance was done based on Jaccard’s similarity coefficient, and clustering analysis was performed based on the unweighted pair- group average (UPGMA) method. Polymorphism Information Content (PIC) analysis showed a high degree of polymorphism ranging from 0.76 to 0.92. Cluster analysis showed that 22 sugarcane varieties were divided into two main clusters and four sub-clusters based on their origin.
format	article
author	RINA SRI KASIAMDARI Ganies Riza Aristya HERI PRABOWO MUHAMMAD FAUZI ARIF Christy Ariesta
author_facet	RINA SRI KASIAMDARI Ganies Riza Aristya HERI PRABOWO MUHAMMAD FAUZI ARIF Christy Ariesta
author_sort	RINA SRI KASIAMDARI
title	Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers
title_short	Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers
title_full	Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers
title_fullStr	Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers
title_full_unstemmed	Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers
title_sort	short communication: genetic diversity of indonesian’s sugarcane varieties (saccharum officinarum) based on rapd molecular markers
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/e1248736af6b49e7996ca2db0748386c
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Short Communication: Genetic diversity of Indonesian’s sugarcane varieties (Saccharum officinarum) based on RAPD molecular markers

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