Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.

<h4>Background</h4>Telomere length (TL) in peripheral blood mononuclear cells (PBMC) from fresh venous blood is increasingly used to estimate molecular impacts of accumulated social adversity on population health. Sometimes, TL extracted from saliva or dried blood spots (DBS) are substit...

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Autores principales:	Arline T Geronimus, John Bound, Colter Mitchell, Aresha Martinez-Cardoso, Linnea Evans, Landon Hughes, Lisa Schneper, Daniel A Notterman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e125aa2727d841f1b36568722bd0480f
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spelling	oai:doaj.org-article:e125aa2727d841f1b36568722bd0480f2021-12-02T20:14:59ZComing up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.1932-620310.1371/journal.pone.0255237https://doaj.org/article/e125aa2727d841f1b36568722bd0480f2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255237https://doaj.org/toc/1932-6203<h4>Background</h4>Telomere length (TL) in peripheral blood mononuclear cells (PBMC) from fresh venous blood is increasingly used to estimate molecular impacts of accumulated social adversity on population health. Sometimes, TL extracted from saliva or dried blood spots (DBS) are substituted as less invasive and more scalable specimen collection methods; yet, are they interchangeable with fresh blood? Studies find TL is correlated across tissues, but have not addressed the critical question for social epidemiological applications: Do different specimen types show the same association between TL and social constructs?<h4>Methods</h4>We integrate expertise in social epidemiology, molecular biology, and the statistical impact of measurement error on parameter estimates. Recruiting a diverse sample of 132 Metro-Detroit women, we measure TL for each woman from fresh blood PBMC, DBS, and saliva. Using regression methods, we estimate associations between social characteristics and TL, comparing estimates across specimen types for each woman.<h4>Results</h4>Associations between TL and social characteristics vary by specimen type collected from the same woman, sometimes qualitatively altering estimates of the magnitude or direction of a theorized relationship. Being Black is associated with shorter TL in PBMC, but longer TL in saliva or DBS. Education is positively associated with TL in fresh blood, but negatively associated with TL using DBS.<h4>Conclusion</h4>Findings raise concerns about the use of TL measures derived from different tissues in social epidemiological research. Investigators need to consider the possibility that associations between social variables and TL may be systematically related to specimen type, rather than be valid indicators of socially-patterned biopsychosocial processes.Arline T GeronimusJohn BoundColter MitchellAresha Martinez-CardosoLinnea EvansLandon HughesLisa SchneperDaniel A NottermanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255237 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arline T Geronimus John Bound Colter Mitchell Aresha Martinez-Cardoso Linnea Evans Landon Hughes Lisa Schneper Daniel A Notterman Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
description	<h4>Background</h4>Telomere length (TL) in peripheral blood mononuclear cells (PBMC) from fresh venous blood is increasingly used to estimate molecular impacts of accumulated social adversity on population health. Sometimes, TL extracted from saliva or dried blood spots (DBS) are substituted as less invasive and more scalable specimen collection methods; yet, are they interchangeable with fresh blood? Studies find TL is correlated across tissues, but have not addressed the critical question for social epidemiological applications: Do different specimen types show the same association between TL and social constructs?<h4>Methods</h4>We integrate expertise in social epidemiology, molecular biology, and the statistical impact of measurement error on parameter estimates. Recruiting a diverse sample of 132 Metro-Detroit women, we measure TL for each woman from fresh blood PBMC, DBS, and saliva. Using regression methods, we estimate associations between social characteristics and TL, comparing estimates across specimen types for each woman.<h4>Results</h4>Associations between TL and social characteristics vary by specimen type collected from the same woman, sometimes qualitatively altering estimates of the magnitude or direction of a theorized relationship. Being Black is associated with shorter TL in PBMC, but longer TL in saliva or DBS. Education is positively associated with TL in fresh blood, but negatively associated with TL using DBS.<h4>Conclusion</h4>Findings raise concerns about the use of TL measures derived from different tissues in social epidemiological research. Investigators need to consider the possibility that associations between social variables and TL may be systematically related to specimen type, rather than be valid indicators of socially-patterned biopsychosocial processes.
format	article
author	Arline T Geronimus John Bound Colter Mitchell Aresha Martinez-Cardoso Linnea Evans Landon Hughes Lisa Schneper Daniel A Notterman
author_facet	Arline T Geronimus John Bound Colter Mitchell Aresha Martinez-Cardoso Linnea Evans Landon Hughes Lisa Schneper Daniel A Notterman
author_sort	Arline T Geronimus
title	Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
title_short	Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
title_full	Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
title_fullStr	Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
title_full_unstemmed	Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
title_sort	coming up short: comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/e125aa2727d841f1b36568722bd0480f
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Coming up short: Comparing venous blood, dried blood spots & saliva samples for measuring telomere length in health equity research.

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