Optimal proteome allocation and the temperature dependence of microbial growth laws

Abstract Although the effect of temperature on microbial growth has been widely studied, the role of proteome allocation in bringing about temperature-induced changes remains elusive. To tackle this problem, we propose a coarse-grained model of microbial growth, including the processes of temperatur...

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Autores principales:	Francis Mairet, Jean-Luc Gouzé, Hidde de Jong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e12831b700494fe4a6382dd00152f3d6
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spelling	oai:doaj.org-article:e12831b700494fe4a6382dd00152f3d62021-12-02T13:30:12ZOptimal proteome allocation and the temperature dependence of microbial growth laws10.1038/s41540-021-00172-y2056-7189https://doaj.org/article/e12831b700494fe4a6382dd00152f3d62021-03-01T00:00:00Zhttps://doi.org/10.1038/s41540-021-00172-yhttps://doaj.org/toc/2056-7189Abstract Although the effect of temperature on microbial growth has been widely studied, the role of proteome allocation in bringing about temperature-induced changes remains elusive. To tackle this problem, we propose a coarse-grained model of microbial growth, including the processes of temperature-sensitive protein unfolding and chaperone-assisted (re)folding. We determine the proteome sector allocation that maximizes balanced growth rate as a function of nutrient limitation and temperature. Calibrated with quantitative proteomic data for Escherichia coli, the model allows us to clarify general principles of temperature-dependent proteome allocation and formulate generalized growth laws. The same activation energy for metabolic enzymes and ribosomes leads to an Arrhenius increase in growth rate at constant proteome composition over a large range of temperatures, whereas at extreme temperatures resources are diverted away from growth to chaperone-mediated stress responses. Our approach points at risks and possible remedies for the use of ribosome content to characterize complex ecosystems with temperature variation.Francis MairetJean-Luc GouzéHidde de JongNature PortfolioarticleBiology (General)QH301-705.5ENnpj Systems Biology and Applications, Vol 7, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Francis Mairet Jean-Luc Gouzé Hidde de Jong Optimal proteome allocation and the temperature dependence of microbial growth laws
description	Abstract Although the effect of temperature on microbial growth has been widely studied, the role of proteome allocation in bringing about temperature-induced changes remains elusive. To tackle this problem, we propose a coarse-grained model of microbial growth, including the processes of temperature-sensitive protein unfolding and chaperone-assisted (re)folding. We determine the proteome sector allocation that maximizes balanced growth rate as a function of nutrient limitation and temperature. Calibrated with quantitative proteomic data for Escherichia coli, the model allows us to clarify general principles of temperature-dependent proteome allocation and formulate generalized growth laws. The same activation energy for metabolic enzymes and ribosomes leads to an Arrhenius increase in growth rate at constant proteome composition over a large range of temperatures, whereas at extreme temperatures resources are diverted away from growth to chaperone-mediated stress responses. Our approach points at risks and possible remedies for the use of ribosome content to characterize complex ecosystems with temperature variation.
format	article
author	Francis Mairet Jean-Luc Gouzé Hidde de Jong
author_facet	Francis Mairet Jean-Luc Gouzé Hidde de Jong
author_sort	Francis Mairet
title	Optimal proteome allocation and the temperature dependence of microbial growth laws
title_short	Optimal proteome allocation and the temperature dependence of microbial growth laws
title_full	Optimal proteome allocation and the temperature dependence of microbial growth laws
title_fullStr	Optimal proteome allocation and the temperature dependence of microbial growth laws
title_full_unstemmed	Optimal proteome allocation and the temperature dependence of microbial growth laws
title_sort	optimal proteome allocation and the temperature dependence of microbial growth laws
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e12831b700494fe4a6382dd00152f3d6
work_keys_str_mv	AT francismairet optimalproteomeallocationandthetemperaturedependenceofmicrobialgrowthlaws AT jeanlucgouze optimalproteomeallocationandthetemperaturedependenceofmicrobialgrowthlaws AT hiddedejong optimalproteomeallocationandthetemperaturedependenceofmicrobialgrowthlaws
_version_	1718392946758254592

Optimal proteome allocation and the temperature dependence of microbial growth laws

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