A new ultradian rhythm in mammalian cell dry mass observed by holography

Abstract We have discovered a new 4 h ultradian rhythm that occurs during the interphase of the cell cycle in a wide range of individual mammalian cells, including both primary and transformed cells. The rhythm was detected by holographic lens-free microscopy that follows the histories of the dry ma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lamya Ghenim, Cédric Allier, Patricia Obeid, Lionel Hervé, Jean-Yves Fortin, Maxim Balakirev, Xavier Gidrol
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e12a61f0d4534c1a8aff8174672d07a3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e12a61f0d4534c1a8aff8174672d07a3
record_format	dspace
spelling	oai:doaj.org-article:e12a61f0d4534c1a8aff8174672d07a32021-12-02T14:12:45ZA new ultradian rhythm in mammalian cell dry mass observed by holography10.1038/s41598-020-79661-92045-2322https://doaj.org/article/e12a61f0d4534c1a8aff8174672d07a32021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79661-9https://doaj.org/toc/2045-2322Abstract We have discovered a new 4 h ultradian rhythm that occurs during the interphase of the cell cycle in a wide range of individual mammalian cells, including both primary and transformed cells. The rhythm was detected by holographic lens-free microscopy that follows the histories of the dry mass of thousands of single live cells simultaneously, each at a resolution of five minutes. It was vital that the rhythm was observed in inherently heterogeneous cell populations, thus eliminating synchronization and labeling bias. The rhythm is independent of circadian rhythm, and is temperature-compensated. We show that the amplitude of the fundamental frequency provides a way to quantify the effects of, chemical reagents on cells, thus shedding light on its mechanism. The rhythm is suppressed by proteostasis disruptors and is detected only in proliferating cells, suggesting that it represents a massive degradation and re-synthesis of protein every 4 h in growing cells.Lamya GhenimCédric AllierPatricia ObeidLionel HervéJean-Yves FortinMaxim BalakirevXavier GidrolNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lamya Ghenim Cédric Allier Patricia Obeid Lionel Hervé Jean-Yves Fortin Maxim Balakirev Xavier Gidrol A new ultradian rhythm in mammalian cell dry mass observed by holography
description	Abstract We have discovered a new 4 h ultradian rhythm that occurs during the interphase of the cell cycle in a wide range of individual mammalian cells, including both primary and transformed cells. The rhythm was detected by holographic lens-free microscopy that follows the histories of the dry mass of thousands of single live cells simultaneously, each at a resolution of five minutes. It was vital that the rhythm was observed in inherently heterogeneous cell populations, thus eliminating synchronization and labeling bias. The rhythm is independent of circadian rhythm, and is temperature-compensated. We show that the amplitude of the fundamental frequency provides a way to quantify the effects of, chemical reagents on cells, thus shedding light on its mechanism. The rhythm is suppressed by proteostasis disruptors and is detected only in proliferating cells, suggesting that it represents a massive degradation and re-synthesis of protein every 4 h in growing cells.
format	article
author	Lamya Ghenim Cédric Allier Patricia Obeid Lionel Hervé Jean-Yves Fortin Maxim Balakirev Xavier Gidrol
author_facet	Lamya Ghenim Cédric Allier Patricia Obeid Lionel Hervé Jean-Yves Fortin Maxim Balakirev Xavier Gidrol
author_sort	Lamya Ghenim
title	A new ultradian rhythm in mammalian cell dry mass observed by holography
title_short	A new ultradian rhythm in mammalian cell dry mass observed by holography
title_full	A new ultradian rhythm in mammalian cell dry mass observed by holography
title_fullStr	A new ultradian rhythm in mammalian cell dry mass observed by holography
title_full_unstemmed	A new ultradian rhythm in mammalian cell dry mass observed by holography
title_sort	new ultradian rhythm in mammalian cell dry mass observed by holography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e12a61f0d4534c1a8aff8174672d07a3
work_keys_str_mv	AT lamyaghenim anewultradianrhythminmammaliancelldrymassobservedbyholography AT cedricallier anewultradianrhythminmammaliancelldrymassobservedbyholography AT patriciaobeid anewultradianrhythminmammaliancelldrymassobservedbyholography AT lionelherve anewultradianrhythminmammaliancelldrymassobservedbyholography AT jeanyvesfortin anewultradianrhythminmammaliancelldrymassobservedbyholography AT maximbalakirev anewultradianrhythminmammaliancelldrymassobservedbyholography AT xaviergidrol anewultradianrhythminmammaliancelldrymassobservedbyholography AT lamyaghenim newultradianrhythminmammaliancelldrymassobservedbyholography AT cedricallier newultradianrhythminmammaliancelldrymassobservedbyholography AT patriciaobeid newultradianrhythminmammaliancelldrymassobservedbyholography AT lionelherve newultradianrhythminmammaliancelldrymassobservedbyholography AT jeanyvesfortin newultradianrhythminmammaliancelldrymassobservedbyholography AT maximbalakirev newultradianrhythminmammaliancelldrymassobservedbyholography AT xaviergidrol newultradianrhythminmammaliancelldrymassobservedbyholography
_version_	1718391816783396864

A new ultradian rhythm in mammalian cell dry mass observed by holography

Ejemplares similares