Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>

Sensing and responding to changes in NaCl concentration in hypersaline environments is vital for cell survival. In this paper, we identified and characterized key components of the high-osmolarity glycerol (HOG) signal transduction pathway, which is crucial in sensing hypersaline conditions in the e...

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Autor principal:	Ana Plemenitaš
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	halotolerant/halophilic fungi <i>Hortaea werneckii</i> Wallemia ichthyophaga HOG signal transduction pathway Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e13c0d6570d340f48ca132f7b7d6863b
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spelling	oai:doaj.org-article:e13c0d6570d340f48ca132f7b7d6863b2021-11-25T18:06:35ZSensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>10.3390/jof71109882309-608Xhttps://doaj.org/article/e13c0d6570d340f48ca132f7b7d6863b2021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/988https://doaj.org/toc/2309-608XSensing and responding to changes in NaCl concentration in hypersaline environments is vital for cell survival. In this paper, we identified and characterized key components of the high-osmolarity glycerol (HOG) signal transduction pathway, which is crucial in sensing hypersaline conditions in the extremely halotolerant black yeast <i>Hortaea werneckii</i> and in the obligate halophilic fungus <i>Wallemia ichthyophaga</i>. Both organisms were isolated from solar salterns, their predominating ecological niche. The identified components included homologous proteins of both branches involved in sensing high osmolarity (SHO1 and SLN1) and the homologues of mitogen-activated protein kinase module (MAPKKK Ste11, MAPKK Pbs2, and MAPK Hog1). Functional complementation of the identified gene products in <i>S. cerevisiae</i> mutant strains revealed some of their functions. Structural protein analysis demonstrated important structural differences in the HOG pathway components between halotolerant/halophilic fungi isolated from solar salterns, salt-sensitive <i>S. cerevisiae</i>, the extremely salt-tolerant <i>H. werneckii</i>, and halophilic <i>W. ichthyophaga</i>. Known and novel gene targets of MAP kinase Hog1 were uncovered particularly in halotolerant <i>H. werneckii.</i> Molecular studies of many salt-responsive proteins confirm unique and novel mechanisms of adaptation to changes in salt concentration.Ana PlemenitašMDPI AGarticlehalotolerant/halophilic fungi<i>Hortaea werneckii</i>Wallemia ichthyophagaHOGsignal transduction pathwayBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 988, p 988 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	halotolerant/halophilic fungi <i>Hortaea werneckii</i> Wallemia ichthyophaga HOG signal transduction pathway Biology (General) QH301-705.5
spellingShingle	halotolerant/halophilic fungi <i>Hortaea werneckii</i> Wallemia ichthyophaga HOG signal transduction pathway Biology (General) QH301-705.5 Ana Plemenitaš Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>
description	Sensing and responding to changes in NaCl concentration in hypersaline environments is vital for cell survival. In this paper, we identified and characterized key components of the high-osmolarity glycerol (HOG) signal transduction pathway, which is crucial in sensing hypersaline conditions in the extremely halotolerant black yeast <i>Hortaea werneckii</i> and in the obligate halophilic fungus <i>Wallemia ichthyophaga</i>. Both organisms were isolated from solar salterns, their predominating ecological niche. The identified components included homologous proteins of both branches involved in sensing high osmolarity (SHO1 and SLN1) and the homologues of mitogen-activated protein kinase module (MAPKKK Ste11, MAPKK Pbs2, and MAPK Hog1). Functional complementation of the identified gene products in <i>S. cerevisiae</i> mutant strains revealed some of their functions. Structural protein analysis demonstrated important structural differences in the HOG pathway components between halotolerant/halophilic fungi isolated from solar salterns, salt-sensitive <i>S. cerevisiae</i>, the extremely salt-tolerant <i>H. werneckii</i>, and halophilic <i>W. ichthyophaga</i>. Known and novel gene targets of MAP kinase Hog1 were uncovered particularly in halotolerant <i>H. werneckii.</i> Molecular studies of many salt-responsive proteins confirm unique and novel mechanisms of adaptation to changes in salt concentration.
format	article
author	Ana Plemenitaš
author_facet	Ana Plemenitaš
author_sort	Ana Plemenitaš
title	Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>
title_short	Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>
title_full	Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>
title_fullStr	Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>
title_full_unstemmed	Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>
title_sort	sensing and responding to hypersaline conditions and the hog signal transduction pathway in fungi isolated from hypersaline environments: <i>hortaea werneckii</i> and <i>wallemia ichthyophaga</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e13c0d6570d340f48ca132f7b7d6863b
work_keys_str_mv	AT anaplemenitas sensingandrespondingtohypersalineconditionsandthehogsignaltransductionpathwayinfungiisolatedfromhypersalineenvironmentsihortaeawerneckiiiandiwallemiaichthyophagai
_version_	1718411651611361280

Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments: <i>Hortaea werneckii</i> and <i>Wallemia ichthyophaga</i>

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