YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus

Abstract The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the prot...

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Autores principales:	Lucía Brown, Josefina M. Villegas, Mariano Elean, Silvina Fadda, Fernanda Mozzi, Lucila Saavedra, Elvira M. Hebert
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1454c1049444b1085e58a8a95fe73ff
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spelling	oai:doaj.org-article:e1454c1049444b1085e58a8a95fe73ff2021-12-02T15:05:32ZYebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus10.1038/s41598-017-09124-12045-2322https://doaj.org/article/e1454c1049444b1085e58a8a95fe73ff2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09124-1https://doaj.org/toc/2045-2322Abstract The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp. lactis CRL 581 was performed. Genes encoding the cell envelope-associated proteinase, two peptide transport systems, and sixteen peptidases were identified. The influence of the peptide supply on the transcription of 23 genes involved in the proteolytic system of L. delbrueckii subsp. lactis was examined after cell growth in a chemically defined medium (CDM) and CDM supplemented with Casitone. prtL, oppA 1, optS, optA genes as well as oppDFBC and optBCDF operons were the most highly expressed genes in CDM; their expression being repressed 6- to 115-fold by the addition of peptides. The transcriptional analysis was confirmed by proteomics; the up-regulation of the PrtL, PepG, OppD and OptF proteins in the absence of peptides was observed while the DNA-binding protein YebC was up-regulated by peptides. Binding of YebC to the promoter region of prtL, oppA 1, and optS, demonstrated by electrophoretic mobility shift assays, showed that YebC acts as a transcriptional repressor of key proteolytic genes.Lucía BrownJosefina M. VillegasMariano EleanSilvina FaddaFernanda MozziLucila SaavedraElvira M. HebertNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lucía Brown Josefina M. Villegas Mariano Elean Silvina Fadda Fernanda Mozzi Lucila Saavedra Elvira M. Hebert YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
description	Abstract The proteolytic system of Lactobacillus plays an essential role in bacterial growth, contributes to the flavor development of fermented products, and can release bioactive health-beneficial peptides during milk fermentation. In this work, a genomic analysis of all genes involved in the proteolytic system of L. delbrueckii subsp. lactis CRL 581 was performed. Genes encoding the cell envelope-associated proteinase, two peptide transport systems, and sixteen peptidases were identified. The influence of the peptide supply on the transcription of 23 genes involved in the proteolytic system of L. delbrueckii subsp. lactis was examined after cell growth in a chemically defined medium (CDM) and CDM supplemented with Casitone. prtL, oppA 1, optS, optA genes as well as oppDFBC and optBCDF operons were the most highly expressed genes in CDM; their expression being repressed 6- to 115-fold by the addition of peptides. The transcriptional analysis was confirmed by proteomics; the up-regulation of the PrtL, PepG, OppD and OptF proteins in the absence of peptides was observed while the DNA-binding protein YebC was up-regulated by peptides. Binding of YebC to the promoter region of prtL, oppA 1, and optS, demonstrated by electrophoretic mobility shift assays, showed that YebC acts as a transcriptional repressor of key proteolytic genes.
format	article
author	Lucía Brown Josefina M. Villegas Mariano Elean Silvina Fadda Fernanda Mozzi Lucila Saavedra Elvira M. Hebert
author_facet	Lucía Brown Josefina M. Villegas Mariano Elean Silvina Fadda Fernanda Mozzi Lucila Saavedra Elvira M. Hebert
author_sort	Lucía Brown
title	YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
title_short	YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
title_full	YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
title_fullStr	YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
title_full_unstemmed	YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus
title_sort	yebc, a putative transcriptional factor involved in the regulation of the proteolytic system of lactobacillus
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e1454c1049444b1085e58a8a95fe73ff
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YebC, a putative transcriptional factor involved in the regulation of the proteolytic system of Lactobacillus

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