Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.

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Detalles Bibliográficos
Autores principales:	Duy P. Nguyen, Yuichiro Miyaoka, Luke A. Gilbert, Steven J. Mayerl, Brian H. Lee, Jonathan S. Weissman, Bruce R. Conklin, James A. Wells
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e153d09732764e8691f331233833775d
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spelling	oai:doaj.org-article:e153d09732764e8691f331233833775d2021-12-02T16:50:55ZLigand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity10.1038/ncomms120092041-1723https://doaj.org/article/e153d09732764e8691f331233833775d2016-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms12009https://doaj.org/toc/2041-1723CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.Duy P. NguyenYuichiro MiyaokaLuke A. GilbertSteven J. MayerlBrian H. LeeJonathan S. WeissmanBruce R. ConklinJames A. WellsNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-10 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Duy P. Nguyen Yuichiro Miyaoka Luke A. Gilbert Steven J. Mayerl Brian H. Lee Jonathan S. Weissman Bruce R. Conklin James A. Wells Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
description	CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.
format	article
author	Duy P. Nguyen Yuichiro Miyaoka Luke A. Gilbert Steven J. Mayerl Brian H. Lee Jonathan S. Weissman Bruce R. Conklin James A. Wells
author_facet	Duy P. Nguyen Yuichiro Miyaoka Luke A. Gilbert Steven J. Mayerl Brian H. Lee Jonathan S. Weissman Bruce R. Conklin James A. Wells
author_sort	Duy P. Nguyen
title	Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
title_short	Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
title_full	Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
title_fullStr	Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
title_full_unstemmed	Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity
title_sort	ligand-binding domains of nuclear receptors facilitate tight control of split crispr activity
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/e153d09732764e8691f331233833775d
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Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

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