Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

Ligand-binding domains of nuclear receptors facilitate tight control of split CRISPR activity

CRISPR-Cas9 has been widely used to manipulate genomes, however control over activity is necessary to explore transcriptional or epigenetic regulation. Here the authors use a split Cas9 fused to nuclear receptor ligand-binding domains to achieve tuneable Cas9 activity.

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Detalles Bibliográficos
Autores principales: Duy P. Nguyen, Yuichiro Miyaoka, Luke A. Gilbert, Steven J. Mayerl, Brian H. Lee, Jonathan S. Weissman, Bruce R. Conklin, James A. Wells
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
Materias:
Science
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Acceso en línea:https://doaj.org/article/e153d09732764e8691f331233833775d
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