The effect of rosuvastatin in a murine model of influenza A infection.

<h4>Rationale</h4>HMG-CoA reductase inhibitors such as rosuvastatin may have immunomodulatory and anti-inflammatory effects that may reduce the severity of influenza A infection. We hypothesized that rosuvastatin would decrease viral replication, attenuate lung injury, and improve mortal...

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Autores principales:	Kathryn A Radigan, Daniela Urich, Alexander V Misharin, Sergio E Chiarella, Saul Soberanes, Angel Gonzalez, Harris Perlman, Richard G Wunderink, G R Scott Budinger, Gökhan M Mutlu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1610579da42473e908243a65e720c16
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spelling	oai:doaj.org-article:e1610579da42473e908243a65e720c162021-11-18T07:21:22ZThe effect of rosuvastatin in a murine model of influenza A infection.1932-620310.1371/journal.pone.0035788https://doaj.org/article/e1610579da42473e908243a65e720c162012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22536437/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Rationale</h4>HMG-CoA reductase inhibitors such as rosuvastatin may have immunomodulatory and anti-inflammatory effects that may reduce the severity of influenza A infection. We hypothesized that rosuvastatin would decrease viral replication, attenuate lung injury, and improve mortality following influenza A infection in mice.<h4>Methods</h4>C57Bl/6 mice were treated daily with rosuvastatin (10 mg/kg/day) supplemented in chow (or control chow) beginning three days prior to infection with either A//Udorn/72 [H3N2] or A/WSN/33 [H1N1] influenza A virus (1×10(5) pfu/mouse). Plaque assays were used to examine the effect of rosuvastatin on viral replication in vitro and in the lungs of infected mice. We measured cell count with differential, protein and cytokines in the bronchoalveolar lavage (BAL) fluid, histologic evidence of lung injury, and wet-to-dry ratio on Day 1, 2, 4, and 6. We also recorded daily weights and mortality.<h4>Results</h4>The administration of rosuvastatin had no effect on viral clearance of influenza A after infection. Weight loss, lung inflammation and lung injury severity were similar in the rosuvastatin and control treated mice. In the mice infected with influenza A (A/WSN/33), mortality was unaffected by treatment with rosuvastatin.<h4>Conclusions</h4>Statins did not alter the replication of influenza A in vitro or enhance its clearance from the lung in vivo. Statins neither attenuated the severity of influenza A-induced lung injury nor had an effect on influenza A-related mortality. Our data suggest that the association between HMG CoA reductase inhibitors and improved outcomes in patients with sepsis and pneumonia are not attributable to their effects on influenza A infection.Kathryn A RadiganDaniela UrichAlexander V MisharinSergio E ChiarellaSaul SoberanesAngel GonzalezHarris PerlmanRichard G WunderinkG R Scott BudingerGökhan M MutluPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35788 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kathryn A Radigan Daniela Urich Alexander V Misharin Sergio E Chiarella Saul Soberanes Angel Gonzalez Harris Perlman Richard G Wunderink G R Scott Budinger Gökhan M Mutlu The effect of rosuvastatin in a murine model of influenza A infection.
description	<h4>Rationale</h4>HMG-CoA reductase inhibitors such as rosuvastatin may have immunomodulatory and anti-inflammatory effects that may reduce the severity of influenza A infection. We hypothesized that rosuvastatin would decrease viral replication, attenuate lung injury, and improve mortality following influenza A infection in mice.<h4>Methods</h4>C57Bl/6 mice were treated daily with rosuvastatin (10 mg/kg/day) supplemented in chow (or control chow) beginning three days prior to infection with either A//Udorn/72 [H3N2] or A/WSN/33 [H1N1] influenza A virus (1×10(5) pfu/mouse). Plaque assays were used to examine the effect of rosuvastatin on viral replication in vitro and in the lungs of infected mice. We measured cell count with differential, protein and cytokines in the bronchoalveolar lavage (BAL) fluid, histologic evidence of lung injury, and wet-to-dry ratio on Day 1, 2, 4, and 6. We also recorded daily weights and mortality.<h4>Results</h4>The administration of rosuvastatin had no effect on viral clearance of influenza A after infection. Weight loss, lung inflammation and lung injury severity were similar in the rosuvastatin and control treated mice. In the mice infected with influenza A (A/WSN/33), mortality was unaffected by treatment with rosuvastatin.<h4>Conclusions</h4>Statins did not alter the replication of influenza A in vitro or enhance its clearance from the lung in vivo. Statins neither attenuated the severity of influenza A-induced lung injury nor had an effect on influenza A-related mortality. Our data suggest that the association between HMG CoA reductase inhibitors and improved outcomes in patients with sepsis and pneumonia are not attributable to their effects on influenza A infection.
format	article
author	Kathryn A Radigan Daniela Urich Alexander V Misharin Sergio E Chiarella Saul Soberanes Angel Gonzalez Harris Perlman Richard G Wunderink G R Scott Budinger Gökhan M Mutlu
author_facet	Kathryn A Radigan Daniela Urich Alexander V Misharin Sergio E Chiarella Saul Soberanes Angel Gonzalez Harris Perlman Richard G Wunderink G R Scott Budinger Gökhan M Mutlu
author_sort	Kathryn A Radigan
title	The effect of rosuvastatin in a murine model of influenza A infection.
title_short	The effect of rosuvastatin in a murine model of influenza A infection.
title_full	The effect of rosuvastatin in a murine model of influenza A infection.
title_fullStr	The effect of rosuvastatin in a murine model of influenza A infection.
title_full_unstemmed	The effect of rosuvastatin in a murine model of influenza A infection.
title_sort	effect of rosuvastatin in a murine model of influenza a infection.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e1610579da42473e908243a65e720c16
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The effect of rosuvastatin in a murine model of influenza A infection.

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