Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway

Bao Hai,1 Yunlong Ma,2 Xiaoyu Pan,1 Lei Yong,1 Chen Liang,1 Guanping He,1 Chenlong Yang,1 Bin Zhu,2 Xiaoguang Liu1 1Department of Orthopedics, Peking University Third Hospital, Beijing 100191, People’s Republic of China; 2The Center for Pain Medicine, Peking University Third Hospital, Bei...

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Autores principales:	Hai B, Ma Y, Pan X, Yong L, Liang C, He G, Yang C, Zhu B, Liu X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Disc degeneration melatonin Atg7 miR-106a-5p Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/e164b29e82fd4a78afc0b915d136cfcd
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spelling	oai:doaj.org-article:e164b29e82fd4a78afc0b915d136cfcd2021-12-02T03:08:49ZMelatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway1178-1998https://doaj.org/article/e164b29e82fd4a78afc0b915d136cfcd2019-03-01T00:00:00Zhttps://www.dovepress.com/melatonin-benefits-to-the-growth-of-human-annulus-fibrosus-cells-throu-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Bao Hai,1 Yunlong Ma,2 Xiaoyu Pan,1 Lei Yong,1 Chen Liang,1 Guanping He,1 Chenlong Yang,1 Bin Zhu,2 Xiaoguang Liu1 1Department of Orthopedics, Peking University Third Hospital, Beijing 100191, People’s Republic of China; 2The Center for Pain Medicine, Peking University Third Hospital, Beijing 100191, People’s Republic of China Background: Disc degeneration (DD) is one of the common diseases worldwide, which deeply influences normal life and leads to excruciating pain. However, an effective treatment for DD is still not identified.Method: The present study systemically examined the effect of melatonin on annulus fibrosus (AF) cells of patients with DD.Results: Melatonin had the effect of promoting proliferation, inducing autophagy, and suppressing apoptosis on AF cells of patients with DD. Moreover, melatonin contributed to the translation and transcription of autophagy-related protein ATG7 and inhibited the function of miR-106a-5p in AF cells. In addition, the results suggested that miR-106a-5p mediated the expression of ATG7 by directly binding to its 3'UTR in AF cells.Conclusion: This research not only gained a deep insight of melatonin mode of action, but also indicated its potential target signaling pathway in AF cells. Keywords: disc degeneration, melatonin, ATG7, miR-106a-5pHai BMa YPan XYong LLiang CHe GYang CZhu BLiu XDove Medical PressarticleDisc degenerationmelatoninAtg7miR-106a-5pGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 14, Pp 621-630 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Disc degeneration melatonin Atg7 miR-106a-5p Geriatrics RC952-954.6
spellingShingle	Disc degeneration melatonin Atg7 miR-106a-5p Geriatrics RC952-954.6 Hai B Ma Y Pan X Yong L Liang C He G Yang C Zhu B Liu X Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
description	Bao Hai,1 Yunlong Ma,2 Xiaoyu Pan,1 Lei Yong,1 Chen Liang,1 Guanping He,1 Chenlong Yang,1 Bin Zhu,2 Xiaoguang Liu1 1Department of Orthopedics, Peking University Third Hospital, Beijing 100191, People’s Republic of China; 2The Center for Pain Medicine, Peking University Third Hospital, Beijing 100191, People’s Republic of China Background: Disc degeneration (DD) is one of the common diseases worldwide, which deeply influences normal life and leads to excruciating pain. However, an effective treatment for DD is still not identified.Method: The present study systemically examined the effect of melatonin on annulus fibrosus (AF) cells of patients with DD.Results: Melatonin had the effect of promoting proliferation, inducing autophagy, and suppressing apoptosis on AF cells of patients with DD. Moreover, melatonin contributed to the translation and transcription of autophagy-related protein ATG7 and inhibited the function of miR-106a-5p in AF cells. In addition, the results suggested that miR-106a-5p mediated the expression of ATG7 by directly binding to its 3'UTR in AF cells.Conclusion: This research not only gained a deep insight of melatonin mode of action, but also indicated its potential target signaling pathway in AF cells. Keywords: disc degeneration, melatonin, ATG7, miR-106a-5p
format	article
author	Hai B Ma Y Pan X Yong L Liang C He G Yang C Zhu B Liu X
author_facet	Hai B Ma Y Pan X Yong L Liang C He G Yang C Zhu B Liu X
author_sort	Hai B
title	Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
title_short	Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
title_full	Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
title_fullStr	Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
title_full_unstemmed	Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
title_sort	melatonin benefits to the growth of human annulus fibrosus cells through inhibiting mir-106a-5p/atg7 signaling pathway
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/e164b29e82fd4a78afc0b915d136cfcd
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Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway

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