Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders

Background Vaginal lubrication is a crucial physiological response that occurs at the beginning of sexual arousal. However, research on lubrication disorders (LD) is still in its infancy, and the role of long non-coding RNAs (lncRNAs) in LD remains unclear. This study aimed to explore the function o...

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Autores principales:	Jingjing Zhang, Jing Zhang, Shengnan Cong, Jingyi Feng, Lianjun Pan, Yuan Zhu, Aixia Zhang, Jiehua Ma
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Female sexual dysfunction Lubrication disorders Long non-coding RNA Co-expression network Medicine R
Acceso en línea:	https://doaj.org/article/e165cd53db5b4281b7d78393fe39816f
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spelling	oai:doaj.org-article:e165cd53db5b4281b7d78393fe39816f2021-11-12T15:05:22ZTranscriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders10.7717/peerj.124852167-8359https://doaj.org/article/e165cd53db5b4281b7d78393fe39816f2021-11-01T00:00:00Zhttps://peerj.com/articles/12485.pdfhttps://peerj.com/articles/12485/https://doaj.org/toc/2167-8359Background Vaginal lubrication is a crucial physiological response that occurs at the beginning of sexual arousal. However, research on lubrication disorders (LD) is still in its infancy, and the role of long non-coding RNAs (lncRNAs) in LD remains unclear. This study aimed to explore the function of lncRNAs in the pathogenesis of vaginal LD. Methods The expression profiles of LD and normal control (NC) lncRNAs were examined using next-generation sequencing (NGS), and eight selected differentially expressed lncRNAs were verified by quantitative real-time PCR. We conducted GO annotation and KEGG pathway enrichment analyses to determine the principal functions of significantly deregulated genes. LncRNA-mRNA co-expression and protein-protein interaction (PPI) networks were constructed and the lncRNA transcription factors (TFs) were predicted. Results From the results, we identified 181,631 lncRNAs and 145,224 mRNAs in vaginal epithelial tissue. Subsequently, our preliminary judgment revealed a total of 499 up-regulated and 337 down-regulated lncRNAs in LD. The top three enriched GO items of the dysregulated lncRNAs included the following significant terms: “contractile fiber part,” “actin filament-based process,” and “contractile fiber”. The most enriched pathways were “cell-extracellular matrix interactions,” “muscle contraction,” “cell-cell communication,” and “cGMP-PKG signaling pathway”. Our results also showed that the lncRNA-mRNA co-expression network was a powerful platform for predicting lncRNA functions. We determined the three hub genes, ADCY5, CXCL12, and NMU, using PPI network construction and analysis. A total of 231 TFs were predicted with RHOXF1, SNAI2, ZNF354C and TBX15 were suspected to be involved in the mechanism of LD. Conclusion In this study, we constructed the lncRNA-mRNA co-expression network, predicted the lncRNA TFs, and comprehensively analyzed lncRNA expression profiles in LD, providing a basis for future studies on LD clinical biomarkers and therapeutic targets. Further research is also needed to fully determine lncRNA’s role in LD development.Jingjing ZhangJing ZhangShengnan CongJingyi FengLianjun PanYuan ZhuAixia ZhangJiehua MaPeerJ Inc.articleFemale sexual dysfunctionLubrication disordersLong non-coding RNACo-expression networkMedicineRENPeerJ, Vol 9, p e12485 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Female sexual dysfunction Lubrication disorders Long non-coding RNA Co-expression network Medicine R
spellingShingle	Female sexual dysfunction Lubrication disorders Long non-coding RNA Co-expression network Medicine R Jingjing Zhang Jing Zhang Shengnan Cong Jingyi Feng Lianjun Pan Yuan Zhu Aixia Zhang Jiehua Ma Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
description	Background Vaginal lubrication is a crucial physiological response that occurs at the beginning of sexual arousal. However, research on lubrication disorders (LD) is still in its infancy, and the role of long non-coding RNAs (lncRNAs) in LD remains unclear. This study aimed to explore the function of lncRNAs in the pathogenesis of vaginal LD. Methods The expression profiles of LD and normal control (NC) lncRNAs were examined using next-generation sequencing (NGS), and eight selected differentially expressed lncRNAs were verified by quantitative real-time PCR. We conducted GO annotation and KEGG pathway enrichment analyses to determine the principal functions of significantly deregulated genes. LncRNA-mRNA co-expression and protein-protein interaction (PPI) networks were constructed and the lncRNA transcription factors (TFs) were predicted. Results From the results, we identified 181,631 lncRNAs and 145,224 mRNAs in vaginal epithelial tissue. Subsequently, our preliminary judgment revealed a total of 499 up-regulated and 337 down-regulated lncRNAs in LD. The top three enriched GO items of the dysregulated lncRNAs included the following significant terms: “contractile fiber part,” “actin filament-based process,” and “contractile fiber”. The most enriched pathways were “cell-extracellular matrix interactions,” “muscle contraction,” “cell-cell communication,” and “cGMP-PKG signaling pathway”. Our results also showed that the lncRNA-mRNA co-expression network was a powerful platform for predicting lncRNA functions. We determined the three hub genes, ADCY5, CXCL12, and NMU, using PPI network construction and analysis. A total of 231 TFs were predicted with RHOXF1, SNAI2, ZNF354C and TBX15 were suspected to be involved in the mechanism of LD. Conclusion In this study, we constructed the lncRNA-mRNA co-expression network, predicted the lncRNA TFs, and comprehensively analyzed lncRNA expression profiles in LD, providing a basis for future studies on LD clinical biomarkers and therapeutic targets. Further research is also needed to fully determine lncRNA’s role in LD development.
format	article
author	Jingjing Zhang Jing Zhang Shengnan Cong Jingyi Feng Lianjun Pan Yuan Zhu Aixia Zhang Jiehua Ma
author_facet	Jingjing Zhang Jing Zhang Shengnan Cong Jingyi Feng Lianjun Pan Yuan Zhu Aixia Zhang Jiehua Ma
author_sort	Jingjing Zhang
title	Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
title_short	Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
title_full	Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
title_fullStr	Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
title_full_unstemmed	Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
title_sort	transcriptome profiling of lncrna and co-expression network in the vaginal epithelial tissue of women with lubrication disorders
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/e165cd53db5b4281b7d78393fe39816f
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Transcriptome profiling of lncRNA and co-expression network in the vaginal epithelial tissue of women with lubrication disorders

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