Phosphoproteomic Comparison of Four Eimeria tenella Life Cycle Stages

Protein phosphorylation is an important post-translational modification (PTM) involved in diverse cellular functions. It is the most prevalent PTM in both Toxoplasma gondii and Plasmodium falciparum, but its status in Eimeria tenella has not b...

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Autores principales:	Xueting Ma, Baohong Liu, Zhenxing Gong, Zigang Qu, Jianping Cai
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Eimeria tenella</i> life cycle phosphoproteomic differentially expressed phosphoproteins (DEPPs) Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e1692cdca50e441a9d4efa266b51af3f
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spelling	oai:doaj.org-article:e1692cdca50e441a9d4efa266b51af3f2021-11-25T17:53:29ZPhosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages10.3390/ijms2222121101422-00671661-6596https://doaj.org/article/e1692cdca50e441a9d4efa266b51af3f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12110https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Protein phosphorylation is an important post-translational modification (PTM) involved in diverse cellular functions. It is the most prevalent PTM in both <i>Toxoplasma gondii</i> and <i>Plasmodium falciparum</i>, but its status in <i>Eimeria tenella</i> has not been reported. Herein, we performed a comprehensive, quantitative phosphoproteomic profile analysis of four stages of the <i>E. tenella</i> life cycle: unsporulated oocysts (USO), partially sporulated (7 h) oocysts (SO7h), sporulated oocysts (SO), and sporozoites (S). A total of 15,247 phosphorylation sites on 9514 phosphopeptides corresponding to 2897 phosphoproteins were identified across the four stages. In addition, 456, 479, and 198 differentially expressed phosphoproteins (DEPPs) were identified in the comparisons SO7h vs. USO, SO vs. SO7h, and S vs. SO, respectively. Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEPPs suggested that they were involved in diverse functions. For SO7h vs. USO, DEPPs were mainly involved in cell division, actin cytoskeleton organization, positive regulation of transport, and pyruvate metabolism. For SO vs. SO7h, they were related to the peptide metabolic process, translation, and RNA transport. DEPPs in the S vs. SO comparison were associated with the tricarboxylic acid metabolic process, positive regulation of ATPase activity, and calcium ion binding. Time course sequencing data analysis (TCseq) identified six clusters with similar expression change characteristics related to carbohydrate metabolism, cytoskeleton organization, and calcium ion transport, demonstrating different regulatory profiles across the life cycle of <i>E. tenella</i>. The results revealed significant changes in the abundance of phosphoproteins during <i>E. tenella</i> development. The findings shed light on the key roles of protein phosphorylation and dephosphorylation in the <i>E. tenella</i> life cycle.Xueting MaBaohong LiuZhenxing GongZigang QuJianping CaiMDPI AGarticle<i>Eimeria tenella</i>life cyclephosphoproteomicdifferentially expressed phosphoproteins (DEPPs)Biology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12110, p 12110 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Eimeria tenella</i> life cycle phosphoproteomic differentially expressed phosphoproteins (DEPPs) Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Eimeria tenella</i> life cycle phosphoproteomic differentially expressed phosphoproteins (DEPPs) Biology (General) QH301-705.5 Chemistry QD1-999 Xueting Ma Baohong Liu Zhenxing Gong Zigang Qu Jianping Cai Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages
description	Protein phosphorylation is an important post-translational modification (PTM) involved in diverse cellular functions. It is the most prevalent PTM in both <i>Toxoplasma gondii</i> and <i>Plasmodium falciparum</i>, but its status in <i>Eimeria tenella</i> has not been reported. Herein, we performed a comprehensive, quantitative phosphoproteomic profile analysis of four stages of the <i>E. tenella</i> life cycle: unsporulated oocysts (USO), partially sporulated (7 h) oocysts (SO7h), sporulated oocysts (SO), and sporozoites (S). A total of 15,247 phosphorylation sites on 9514 phosphopeptides corresponding to 2897 phosphoproteins were identified across the four stages. In addition, 456, 479, and 198 differentially expressed phosphoproteins (DEPPs) were identified in the comparisons SO7h vs. USO, SO vs. SO7h, and S vs. SO, respectively. Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of DEPPs suggested that they were involved in diverse functions. For SO7h vs. USO, DEPPs were mainly involved in cell division, actin cytoskeleton organization, positive regulation of transport, and pyruvate metabolism. For SO vs. SO7h, they were related to the peptide metabolic process, translation, and RNA transport. DEPPs in the S vs. SO comparison were associated with the tricarboxylic acid metabolic process, positive regulation of ATPase activity, and calcium ion binding. Time course sequencing data analysis (TCseq) identified six clusters with similar expression change characteristics related to carbohydrate metabolism, cytoskeleton organization, and calcium ion transport, demonstrating different regulatory profiles across the life cycle of <i>E. tenella</i>. The results revealed significant changes in the abundance of phosphoproteins during <i>E. tenella</i> development. The findings shed light on the key roles of protein phosphorylation and dephosphorylation in the <i>E. tenella</i> life cycle.
format	article
author	Xueting Ma Baohong Liu Zhenxing Gong Zigang Qu Jianping Cai
author_facet	Xueting Ma Baohong Liu Zhenxing Gong Zigang Qu Jianping Cai
author_sort	Xueting Ma
title	Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages
title_short	Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages
title_full	Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages
title_fullStr	Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages
title_full_unstemmed	Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages
title_sort	phosphoproteomic comparison of four <i>eimeria tenella</i> life cycle stages
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e1692cdca50e441a9d4efa266b51af3f
work_keys_str_mv	AT xuetingma phosphoproteomiccomparisonoffourieimeriatenellailifecyclestages AT baohongliu phosphoproteomiccomparisonoffourieimeriatenellailifecyclestages AT zhenxinggong phosphoproteomiccomparisonoffourieimeriatenellailifecyclestages AT zigangqu phosphoproteomiccomparisonoffourieimeriatenellailifecyclestages AT jianpingcai phosphoproteomiccomparisonoffourieimeriatenellailifecyclestages
_version_	1718411893994946560

Phosphoproteomic Comparison of Four <i>Eimeria tenella</i> Life Cycle Stages

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