Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia

Abstract This study aimed to determine whether mRNA and protein levels of cartilage oligomeric matrix protein (COMP), a glycoprotein responsible for modulating homeostasis of extracellular matrix, in the systemic and local liver environments were associated with clinical parameters of biliary atresi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wanvisa Udomsinprasert, Napat Angkathunyakul, Jiraphun Jittikoon, Usa Chaikledkaew, Paisarn Vejchapipat, Yong Poovorawan, Sittisak Honsawek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e169ffbcd1c7489dbb5d185a0ff6c95c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e169ffbcd1c7489dbb5d185a0ff6c95c
record_format	dspace
spelling	oai:doaj.org-article:e169ffbcd1c7489dbb5d185a0ff6c95c2021-12-02T18:51:42ZCartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia10.1038/s41598-021-95805-x2045-2322https://doaj.org/article/e169ffbcd1c7489dbb5d185a0ff6c95c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95805-xhttps://doaj.org/toc/2045-2322Abstract This study aimed to determine whether mRNA and protein levels of cartilage oligomeric matrix protein (COMP), a glycoprotein responsible for modulating homeostasis of extracellular matrix, in the systemic and local liver environments were associated with clinical parameters of biliary atresia (BA) patients and might serve as a biomarker for BA severity. COMP protein levels in the circulation of 96 BA patients and 56 healthy controls and its mRNA and protein expressions in the liver of 20 BA patients and 5 non-BA patients were evaluated using enzyme-linked immunosorbent assay, real-time polymerase chain reaction, and immunohistochemistry, respectively. In the circulation of BA patients, COMP levels were significantly higher than those in healthy controls. Compared with early-stage BA patients, those with advanced-stage including jaundice, fibrosis, and hepatic dysfunction had significantly increased circulating COMP levels. Raised circulating COMP levels were found to be independently correlated with degree of liver fibrosis. Survival analysis showed that elevated circulating COMP levels were significantly associated with decreased survival of BA patients. Receiver-operating characteristic curve analysis unveiled a diagnostic value of circulating COMP as a non-invasive biomarker of BA (AUC = 0.99), with a sensitivity of 100.0% and a specificity of 98.2%. In the liver, both COMP mRNA and protein expressions of BA patients with fibrosis were significantly greater than those of BA patients without fibrosis and non-BA patients. Collectively, increased circulating COMP might reflect unfavorable outcome of BA patients and have potential as a novel biomarker for the disease severity following Kasai-operation.Wanvisa UdomsinprasertNapat AngkathunyakulJiraphun JittikoonUsa ChaikledkaewPaisarn VejchapipatYong PoovorawanSittisak HonsawekNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wanvisa Udomsinprasert Napat Angkathunyakul Jiraphun Jittikoon Usa Chaikledkaew Paisarn Vejchapipat Yong Poovorawan Sittisak Honsawek Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
description	Abstract This study aimed to determine whether mRNA and protein levels of cartilage oligomeric matrix protein (COMP), a glycoprotein responsible for modulating homeostasis of extracellular matrix, in the systemic and local liver environments were associated with clinical parameters of biliary atresia (BA) patients and might serve as a biomarker for BA severity. COMP protein levels in the circulation of 96 BA patients and 56 healthy controls and its mRNA and protein expressions in the liver of 20 BA patients and 5 non-BA patients were evaluated using enzyme-linked immunosorbent assay, real-time polymerase chain reaction, and immunohistochemistry, respectively. In the circulation of BA patients, COMP levels were significantly higher than those in healthy controls. Compared with early-stage BA patients, those with advanced-stage including jaundice, fibrosis, and hepatic dysfunction had significantly increased circulating COMP levels. Raised circulating COMP levels were found to be independently correlated with degree of liver fibrosis. Survival analysis showed that elevated circulating COMP levels were significantly associated with decreased survival of BA patients. Receiver-operating characteristic curve analysis unveiled a diagnostic value of circulating COMP as a non-invasive biomarker of BA (AUC = 0.99), with a sensitivity of 100.0% and a specificity of 98.2%. In the liver, both COMP mRNA and protein expressions of BA patients with fibrosis were significantly greater than those of BA patients without fibrosis and non-BA patients. Collectively, increased circulating COMP might reflect unfavorable outcome of BA patients and have potential as a novel biomarker for the disease severity following Kasai-operation.
format	article
author	Wanvisa Udomsinprasert Napat Angkathunyakul Jiraphun Jittikoon Usa Chaikledkaew Paisarn Vejchapipat Yong Poovorawan Sittisak Honsawek
author_facet	Wanvisa Udomsinprasert Napat Angkathunyakul Jiraphun Jittikoon Usa Chaikledkaew Paisarn Vejchapipat Yong Poovorawan Sittisak Honsawek
author_sort	Wanvisa Udomsinprasert
title	Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
title_short	Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
title_full	Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
title_fullStr	Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
title_full_unstemmed	Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
title_sort	cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e169ffbcd1c7489dbb5d185a0ff6c95c
work_keys_str_mv	AT wanvisaudomsinprasert cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia AT napatangkathunyakul cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia AT jiraphunjittikoon cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia AT usachaikledkaew cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia AT paisarnvejchapipat cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia AT yongpoovorawan cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia AT sittisakhonsawek cartilageoligomericmatrixproteinasamarkerofprogressiveliverfibrosisinbiliaryatresia
_version_	1718377418299801600

Cartilage oligomeric matrix protein as a marker of progressive liver fibrosis in biliary atresia

Ejemplares similares