Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.

Thuricin CD is a two-component bacteriocin produced by Bacillus thuringiensis that kills a wide range of clinically significant Clostridium difficile. This bacteriocin has recently been characterized and consists of two distinct peptides, Trnβ and Trnα, which both possess 3 intrapeptide sulphur to α...

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Autores principales:	Kiera Murphy, Orla O'Sullivan, Mary C Rea, Paul D Cotter, R Paul Ross, Colin Hill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e17376b224fe43bd89bf9b6b355bb21b
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spelling	oai:doaj.org-article:e17376b224fe43bd89bf9b6b355bb21b2021-11-18T06:50:34ZGenome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.1932-620310.1371/journal.pone.0020852https://doaj.org/article/e17376b224fe43bd89bf9b6b355bb21b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21760885/?tool=EBIhttps://doaj.org/toc/1932-6203Thuricin CD is a two-component bacteriocin produced by Bacillus thuringiensis that kills a wide range of clinically significant Clostridium difficile. This bacteriocin has recently been characterized and consists of two distinct peptides, Trnβ and Trnα, which both possess 3 intrapeptide sulphur to α-carbon bridges and act synergistically. Indeed, thuricin CD and subtilosin A are the only antimicrobials known to possess these unusual structures and are known as the sactibiotics (sulplur to alpha carbon-containing antibiotics). Analysis of the thuricin CD-associated gene cluster revealed the presence of genes encoding two highly unusual SAM proteins (TrnC and TrnD) which are proposed to be responsible for these unusual post-translational modifications. On the basis of the frequently high conservation among enzymes responsible for the post-translational modification of specific antimicrobials, we performed an in silico screen for novel thuricin CD-like gene clusters using the TrnC and TrnD radical SAM proteins as driver sequences to perform an initial homology search against the complete non-redundant database. Fifteen novel thuricin CD-like gene clusters were identified, based on the presence of TrnC and TrnD homologues in the context of neighbouring genes encoding potential bacteriocin structural peptides. Moreover, metagenomic analysis revealed that TrnC or TrnD homologs are present in a variety of metagenomic environments, suggesting a widespread distribution of thuricin-like operons in a variety of environments. In-silico analysis of radical SAM proteins is sufficient to identify novel putative sactibiotic clusters.Kiera MurphyOrla O'SullivanMary C ReaPaul D CotterR Paul RossColin HillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e20852 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kiera Murphy Orla O'Sullivan Mary C Rea Paul D Cotter R Paul Ross Colin Hill Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.
description	Thuricin CD is a two-component bacteriocin produced by Bacillus thuringiensis that kills a wide range of clinically significant Clostridium difficile. This bacteriocin has recently been characterized and consists of two distinct peptides, Trnβ and Trnα, which both possess 3 intrapeptide sulphur to α-carbon bridges and act synergistically. Indeed, thuricin CD and subtilosin A are the only antimicrobials known to possess these unusual structures and are known as the sactibiotics (sulplur to alpha carbon-containing antibiotics). Analysis of the thuricin CD-associated gene cluster revealed the presence of genes encoding two highly unusual SAM proteins (TrnC and TrnD) which are proposed to be responsible for these unusual post-translational modifications. On the basis of the frequently high conservation among enzymes responsible for the post-translational modification of specific antimicrobials, we performed an in silico screen for novel thuricin CD-like gene clusters using the TrnC and TrnD radical SAM proteins as driver sequences to perform an initial homology search against the complete non-redundant database. Fifteen novel thuricin CD-like gene clusters were identified, based on the presence of TrnC and TrnD homologues in the context of neighbouring genes encoding potential bacteriocin structural peptides. Moreover, metagenomic analysis revealed that TrnC or TrnD homologs are present in a variety of metagenomic environments, suggesting a widespread distribution of thuricin-like operons in a variety of environments. In-silico analysis of radical SAM proteins is sufficient to identify novel putative sactibiotic clusters.
format	article
author	Kiera Murphy Orla O'Sullivan Mary C Rea Paul D Cotter R Paul Ross Colin Hill
author_facet	Kiera Murphy Orla O'Sullivan Mary C Rea Paul D Cotter R Paul Ross Colin Hill
author_sort	Kiera Murphy
title	Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.
title_short	Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.
title_full	Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.
title_fullStr	Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.
title_full_unstemmed	Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.
title_sort	genome mining for radical sam protein determinants reveals multiple sactibiotic-like gene clusters.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e17376b224fe43bd89bf9b6b355bb21b
work_keys_str_mv	AT kieramurphy genomeminingforradicalsamproteindeterminantsrevealsmultiplesactibioticlikegeneclusters AT orlaosullivan genomeminingforradicalsamproteindeterminantsrevealsmultiplesactibioticlikegeneclusters AT marycrea genomeminingforradicalsamproteindeterminantsrevealsmultiplesactibioticlikegeneclusters AT pauldcotter genomeminingforradicalsamproteindeterminantsrevealsmultiplesactibioticlikegeneclusters AT rpaulross genomeminingforradicalsamproteindeterminantsrevealsmultiplesactibioticlikegeneclusters AT colinhill genomeminingforradicalsamproteindeterminantsrevealsmultiplesactibioticlikegeneclusters
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Genome mining for radical SAM protein determinants reveals multiple sactibiotic-like gene clusters.

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