Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP

Yulita KS, Ridwan. 2018. Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP. Biodiversitas 19: 1160-1168. Jewawut (Setaria italica ssp. italica) or foxtail millet is minor crops domesticated and consumed only in few parts o...

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Autores principales:	KUSUMADEWI SRI YULITA, RIDWAN RIDWAN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2018
Materias:	foxtail millet gamma rays jewawut mutation srap Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e173d2c0a3df4215beb91f9538e9c68e
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spelling	oai:doaj.org-article:e173d2c0a3df4215beb91f9538e9c68e2021-11-16T14:03:00ZMolecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP1412-033X2085-472210.13057/biodiv/d190352https://doaj.org/article/e173d2c0a3df4215beb91f9538e9c68e2018-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/2761https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Yulita KS, Ridwan. 2018. Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP. Biodiversitas 19: 1160-1168. Jewawut (Setaria italica ssp. italica) or foxtail millet is minor crops domesticated and consumed only in few parts of Sulawesi, Maluku and East Nusa Tenggara, while in other region such as Java, it is known as food for bird despite the fact that jewawut is rich in fiber. The utilization of jewawut as an alternative crop was limited due to their small size of seeds as well low yield. An effort to improve the potency of jewawut as an alternative food is through genetic improvement by induced mutation. This present study was aimed to determinate mutant of jewawut from Buru Island irradiated by gamma rays as potential genotype to produce high grain yield. Two variants of jewawut were recorded from Buru Island, red and yellow variant each referring to the colour of their seeds. Samples were consisted of 26 red and 24 of yellow variant. Mutant was detected by molecular characterization using SRAP marker. UPGMA cluster analysis was also performed to ensure the position of mutant within the UPGMA dendrogram against the control samples. Mutant with the most distinct genyotypes for yellow variant are accession O1O2#10 treated in 100 Gy and O1E1#10 treated in 200 Gy gamma radiation, whereas in the red variant are accessions O2C4#13 treated in 50 Gy and O2E4#6 treated in 200 Gy gamma radiation. All the mutants have random agronomic performances relative to controls. Mutants with potential high grain yield are O2B2#14 treated in 25 Gy for the red variant, and this should be used as a candidate to develop superior genotype.KUSUMADEWI SRI YULITARIDWAN RIDWANMBI & UNS Soloarticlefoxtail milletgamma raysjewawutmutationsrapBiology (General)QH301-705.5ENBiodiversitas, Vol 19, Iss 3, Pp 1160-1168 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	foxtail millet gamma rays jewawut mutation srap Biology (General) QH301-705.5
spellingShingle	foxtail millet gamma rays jewawut mutation srap Biology (General) QH301-705.5 KUSUMADEWI SRI YULITA RIDWAN RIDWAN Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP
description	Yulita KS, Ridwan. 2018. Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP. Biodiversitas 19: 1160-1168. Jewawut (Setaria italica ssp. italica) or foxtail millet is minor crops domesticated and consumed only in few parts of Sulawesi, Maluku and East Nusa Tenggara, while in other region such as Java, it is known as food for bird despite the fact that jewawut is rich in fiber. The utilization of jewawut as an alternative crop was limited due to their small size of seeds as well low yield. An effort to improve the potency of jewawut as an alternative food is through genetic improvement by induced mutation. This present study was aimed to determinate mutant of jewawut from Buru Island irradiated by gamma rays as potential genotype to produce high grain yield. Two variants of jewawut were recorded from Buru Island, red and yellow variant each referring to the colour of their seeds. Samples were consisted of 26 red and 24 of yellow variant. Mutant was detected by molecular characterization using SRAP marker. UPGMA cluster analysis was also performed to ensure the position of mutant within the UPGMA dendrogram against the control samples. Mutant with the most distinct genyotypes for yellow variant are accession O1O2#10 treated in 100 Gy and O1E1#10 treated in 200 Gy gamma radiation, whereas in the red variant are accessions O2C4#13 treated in 50 Gy and O2E4#6 treated in 200 Gy gamma radiation. All the mutants have random agronomic performances relative to controls. Mutants with potential high grain yield are O2B2#14 treated in 25 Gy for the red variant, and this should be used as a candidate to develop superior genotype.
format	article
author	KUSUMADEWI SRI YULITA RIDWAN RIDWAN
author_facet	KUSUMADEWI SRI YULITA RIDWAN RIDWAN
author_sort	KUSUMADEWI SRI YULITA
title	Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP
title_short	Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP
title_full	Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP
title_fullStr	Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP
title_full_unstemmed	Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP
title_sort	molecular characterization of induced mutation of jewawut (setaria italica ssp. italica) from buru island, indonesia using srap
publisher	MBI & UNS Solo
publishDate	2018
url	https://doaj.org/article/e173d2c0a3df4215beb91f9538e9c68e
work_keys_str_mv	AT kusumadewisriyulita molecularcharacterizationofinducedmutationofjewawutsetariaitalicasspitalicafromburuislandindonesiausingsrap AT ridwanridwan molecularcharacterizationofinducedmutationofjewawutsetariaitalicasspitalicafromburuislandindonesiausingsrap
_version_	1718426454231875584

Molecular characterization of induced mutation of jewawut (Setaria italica ssp. italica) from Buru Island, Indonesia using SRAP

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