Dengue virus infection increases microglial cell migration

Abstract Activated microglial cells are present in dengue virus (DENV)-infected brains; however, the possible effects of DENV on microglia remain unclear. Here, we demonstrated DENV caused infection, including viral entry, RNA replication, viral protein expression, and virus release, in the murine m...

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Autores principales:	Ming-Kai Jhan, Tsung-Ting Tsai, Chia-Ling Chen, Cheng-Chieh Tsai, Yi-Lin Cheng, Yi-Chao Lee, Chiung-Yuan Ko, Yee-Shin Lin, Chih-Peng Chang, Liang-Tzung Lin, Chiou-Feng Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e17c7c248ff54c3b94756749756ab941
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spelling	oai:doaj.org-article:e17c7c248ff54c3b94756749756ab9412021-12-02T15:06:13ZDengue virus infection increases microglial cell migration10.1038/s41598-017-00182-z2045-2322https://doaj.org/article/e17c7c248ff54c3b94756749756ab9412017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00182-zhttps://doaj.org/toc/2045-2322Abstract Activated microglial cells are present in dengue virus (DENV)-infected brains; however, the possible effects of DENV on microglia remain unclear. Here, we demonstrated DENV caused infection, including viral entry, RNA replication, viral protein expression, and virus release, in the murine microglial cell line BV2. DENV infection caused an increase in the formation of the multipolar phenotype in vitro and in vivo without affecting cell growth and cytotoxicity. DENV infection considerably increased cell motility and disrupting either actin filaments or clathrin retarded such effect. Increase in cell migration was only occurred by DENV infection following a clathrin-regulated endocytosis of DENV entry. Ultraviolet-inactivated DENV did not affect cell migration, and pharmacologically blocking toll-like receptor (TLR) 3 and TLR3-related signaling pathways reduced the DENV-induced increase in cell migration. These results demonstrate an advanced effect of DENV infection on microglial migration via a mechanism involving viral entry, RNA release, and TLR3 signal activation.Ming-Kai JhanTsung-Ting TsaiChia-Ling ChenCheng-Chieh TsaiYi-Lin ChengYi-Chao LeeChiung-Yuan KoYee-Shin LinChih-Peng ChangLiang-Tzung LinChiou-Feng LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ming-Kai Jhan Tsung-Ting Tsai Chia-Ling Chen Cheng-Chieh Tsai Yi-Lin Cheng Yi-Chao Lee Chiung-Yuan Ko Yee-Shin Lin Chih-Peng Chang Liang-Tzung Lin Chiou-Feng Lin Dengue virus infection increases microglial cell migration
description	Abstract Activated microglial cells are present in dengue virus (DENV)-infected brains; however, the possible effects of DENV on microglia remain unclear. Here, we demonstrated DENV caused infection, including viral entry, RNA replication, viral protein expression, and virus release, in the murine microglial cell line BV2. DENV infection caused an increase in the formation of the multipolar phenotype in vitro and in vivo without affecting cell growth and cytotoxicity. DENV infection considerably increased cell motility and disrupting either actin filaments or clathrin retarded such effect. Increase in cell migration was only occurred by DENV infection following a clathrin-regulated endocytosis of DENV entry. Ultraviolet-inactivated DENV did not affect cell migration, and pharmacologically blocking toll-like receptor (TLR) 3 and TLR3-related signaling pathways reduced the DENV-induced increase in cell migration. These results demonstrate an advanced effect of DENV infection on microglial migration via a mechanism involving viral entry, RNA release, and TLR3 signal activation.
format	article
author	Ming-Kai Jhan Tsung-Ting Tsai Chia-Ling Chen Cheng-Chieh Tsai Yi-Lin Cheng Yi-Chao Lee Chiung-Yuan Ko Yee-Shin Lin Chih-Peng Chang Liang-Tzung Lin Chiou-Feng Lin
author_facet	Ming-Kai Jhan Tsung-Ting Tsai Chia-Ling Chen Cheng-Chieh Tsai Yi-Lin Cheng Yi-Chao Lee Chiung-Yuan Ko Yee-Shin Lin Chih-Peng Chang Liang-Tzung Lin Chiou-Feng Lin
author_sort	Ming-Kai Jhan
title	Dengue virus infection increases microglial cell migration
title_short	Dengue virus infection increases microglial cell migration
title_full	Dengue virus infection increases microglial cell migration
title_fullStr	Dengue virus infection increases microglial cell migration
title_full_unstemmed	Dengue virus infection increases microglial cell migration
title_sort	dengue virus infection increases microglial cell migration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e17c7c248ff54c3b94756749756ab941
work_keys_str_mv	AT mingkaijhan denguevirusinfectionincreasesmicroglialcellmigration AT tsungtingtsai denguevirusinfectionincreasesmicroglialcellmigration AT chialingchen denguevirusinfectionincreasesmicroglialcellmigration AT chengchiehtsai denguevirusinfectionincreasesmicroglialcellmigration AT yilincheng denguevirusinfectionincreasesmicroglialcellmigration AT yichaolee denguevirusinfectionincreasesmicroglialcellmigration AT chiungyuanko denguevirusinfectionincreasesmicroglialcellmigration AT yeeshinlin denguevirusinfectionincreasesmicroglialcellmigration AT chihpengchang denguevirusinfectionincreasesmicroglialcellmigration AT liangtzunglin denguevirusinfectionincreasesmicroglialcellmigration AT chioufenglin denguevirusinfectionincreasesmicroglialcellmigration
_version_	1718388549125931008

Dengue virus infection increases microglial cell migration

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