Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells

Abstract Osteoclasts (OCs) and much less dendritic cells (DCs) induce significant expansion and functional activation of NK cells, and furthermore, the OC-expanded NK cells preferentially increase the expansion and activation of CD8+ T cells by targeting CD4+ T cells. When autologous OCs were used t...

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Autores principales:	Kawaljit Kaur, Meng-Wei Ko, Nick Ohanian, Jessica Cook, Anahid Jewett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e17d77d68b1d4c05bf67756c29ed0972
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spelling	oai:doaj.org-article:e17d77d68b1d4c05bf67756c29ed09722021-12-02T16:08:38ZOsteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells10.1038/s41598-020-76702-12045-2322https://doaj.org/article/e17d77d68b1d4c05bf67756c29ed09722020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-76702-1https://doaj.org/toc/2045-2322Abstract Osteoclasts (OCs) and much less dendritic cells (DCs) induce significant expansion and functional activation of NK cells, and furthermore, the OC-expanded NK cells preferentially increase the expansion and activation of CD8+ T cells by targeting CD4+ T cells. When autologous OCs were used to expand patient NK cells much lower percentages of expanded CD8+ T cells, decreased numbers of expanded NK cells and decreased functions of NK cells could be observed, and the addition of allogeneic healthy OCs increased the patients’ NK function. Mechanistically, OC-expanded NK cells were found to lyse CD4+ T cells but not CD8+ T cells suggesting potential selection of CD8+ T cells before their expansion by OC activated NK cells. In agreement, Increased IFN-γ secretion, and NK cell-mediated cytotoxicity and higher percentages of CD8+ T cells, in various tissue compartments of oral tumor-bearing hu-BLT mice in response to immunotherapy by OC-expanded NK cells were observed. Thus, our results indicate an important relationship between NK and CD8+ T cells.Kawaljit KaurMeng-Wei KoNick OhanianJessica CookAnahid JewettNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-17 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kawaljit Kaur Meng-Wei Ko Nick Ohanian Jessica Cook Anahid Jewett Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells
description	Abstract Osteoclasts (OCs) and much less dendritic cells (DCs) induce significant expansion and functional activation of NK cells, and furthermore, the OC-expanded NK cells preferentially increase the expansion and activation of CD8+ T cells by targeting CD4+ T cells. When autologous OCs were used to expand patient NK cells much lower percentages of expanded CD8+ T cells, decreased numbers of expanded NK cells and decreased functions of NK cells could be observed, and the addition of allogeneic healthy OCs increased the patients’ NK function. Mechanistically, OC-expanded NK cells were found to lyse CD4+ T cells but not CD8+ T cells suggesting potential selection of CD8+ T cells before their expansion by OC activated NK cells. In agreement, Increased IFN-γ secretion, and NK cell-mediated cytotoxicity and higher percentages of CD8+ T cells, in various tissue compartments of oral tumor-bearing hu-BLT mice in response to immunotherapy by OC-expanded NK cells were observed. Thus, our results indicate an important relationship between NK and CD8+ T cells.
format	article
author	Kawaljit Kaur Meng-Wei Ko Nick Ohanian Jessica Cook Anahid Jewett
author_facet	Kawaljit Kaur Meng-Wei Ko Nick Ohanian Jessica Cook Anahid Jewett
author_sort	Kawaljit Kaur
title	Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells
title_short	Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells
title_full	Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells
title_fullStr	Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells
title_full_unstemmed	Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells
title_sort	osteoclast-expanded super-charged nk-cells preferentially select and expand cd8+ t cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e17d77d68b1d4c05bf67756c29ed0972
work_keys_str_mv	AT kawaljitkaur osteoclastexpandedsuperchargednkcellspreferentiallyselectandexpandcd8tcells AT mengweiko osteoclastexpandedsuperchargednkcellspreferentiallyselectandexpandcd8tcells AT nickohanian osteoclastexpandedsuperchargednkcellspreferentiallyselectandexpandcd8tcells AT jessicacook osteoclastexpandedsuperchargednkcellspreferentiallyselectandexpandcd8tcells AT anahidjewett osteoclastexpandedsuperchargednkcellspreferentiallyselectandexpandcd8tcells
_version_	1718384502737207296

Osteoclast-expanded super-charged NK-cells preferentially select and expand CD8+ T cells

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