Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia

Abstract. Sari MA, Retnaningrum E. 2019. Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia. Biodiversitas 20: 3422-3429. High oil mining activities cause environmental pollution at the oil mining site. Petroleum spills...

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Autores principales:	Mutiara Arum Sari, ENDAH RETNANINGRUM
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	16s rrna gene, hydrocarbons biodegradation, phylogenetic tree, pseudomonas aeruginosa, rhizobacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e181f83c0c914fdc9261ae5c87b1291a
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spelling	oai:doaj.org-article:e181f83c0c914fdc9261ae5c87b1291a2021-11-21T21:57:23ZHydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia1412-033X2085-472210.13057/biodiv/d201140https://doaj.org/article/e181f83c0c914fdc9261ae5c87b1291a2019-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3997https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sari MA, Retnaningrum E. 2019. Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia. Biodiversitas 20: 3422-3429. High oil mining activities cause environmental pollution at the oil mining site. Petroleum spills and waste to the river flow cause blackish discoloration along the riverbank and also some types of plants die. The utilization of rhizospheric bacteria (rhizobacteria) as a bioremediation agent is considered as appropriate for reducing petroleum contaminants. The purposes of this study were to analyze the ability of hydrocarbon degradation by selected rhizobacteria isolates, to identify molecularly and analyze the genetic relationship of hydrocarbon-degrading rhizobacteria based on 16S rRNA gene sequencing. The rhizobacteria of cogongrass (Imperata cylindrica L.) were isolated from oil mining site in Wonocolo, Kedewan, Bojonegoro, East Java, Indonesia. The 5 isolates of rhizobacteria were then cultured on Bushnell-Haas Mineral Salt (BHMS) medium with the addition of 0.015% crude oil for analyzing their growth based on their cell densities using the Total Plate Count method. The optimal rhizobacteria growth obtained (MTMW2 isolate) was selected and further analysed its ability for hydrocarbon degradation through measuring of Total Petroleum Hydrocarbon (TPH). Its rhizobacteria were then identified using 16S rRNA gene sequencing. The MTMW2 isolate revealed specific growth rate of 0.14 in BHMS medium with the addition of 0.015% crude oil. After 10 days of incubation, the isolates could degrade Total Petroleum Hydrocarbon in the medium as much as 96.1%. The results of molecular identification and phylogenetic tree construction showed that MTMW2 isolate was identified as Pseudomonas aeruginosa.Mutiara Arum SariENDAH RETNANINGRUMMBI & UNS Soloarticle16s rrna gene, hydrocarbons biodegradation, phylogenetic tree, pseudomonas aeruginosa, rhizobacteriaBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene, hydrocarbons biodegradation, phylogenetic tree, pseudomonas aeruginosa, rhizobacteria Biology (General) QH301-705.5
spellingShingle	16s rrna gene, hydrocarbons biodegradation, phylogenetic tree, pseudomonas aeruginosa, rhizobacteria Biology (General) QH301-705.5 Mutiara Arum Sari ENDAH RETNANINGRUM Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia
description	Abstract. Sari MA, Retnaningrum E. 2019. Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia. Biodiversitas 20: 3422-3429. High oil mining activities cause environmental pollution at the oil mining site. Petroleum spills and waste to the river flow cause blackish discoloration along the riverbank and also some types of plants die. The utilization of rhizospheric bacteria (rhizobacteria) as a bioremediation agent is considered as appropriate for reducing petroleum contaminants. The purposes of this study were to analyze the ability of hydrocarbon degradation by selected rhizobacteria isolates, to identify molecularly and analyze the genetic relationship of hydrocarbon-degrading rhizobacteria based on 16S rRNA gene sequencing. The rhizobacteria of cogongrass (Imperata cylindrica L.) were isolated from oil mining site in Wonocolo, Kedewan, Bojonegoro, East Java, Indonesia. The 5 isolates of rhizobacteria were then cultured on Bushnell-Haas Mineral Salt (BHMS) medium with the addition of 0.015% crude oil for analyzing their growth based on their cell densities using the Total Plate Count method. The optimal rhizobacteria growth obtained (MTMW2 isolate) was selected and further analysed its ability for hydrocarbon degradation through measuring of Total Petroleum Hydrocarbon (TPH). Its rhizobacteria were then identified using 16S rRNA gene sequencing. The MTMW2 isolate revealed specific growth rate of 0.14 in BHMS medium with the addition of 0.015% crude oil. After 10 days of incubation, the isolates could degrade Total Petroleum Hydrocarbon in the medium as much as 96.1%. The results of molecular identification and phylogenetic tree construction showed that MTMW2 isolate was identified as Pseudomonas aeruginosa.
format	article
author	Mutiara Arum Sari ENDAH RETNANINGRUM
author_facet	Mutiara Arum Sari ENDAH RETNANINGRUM
author_sort	Mutiara Arum Sari
title	Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia
title_short	Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia
title_full	Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia
title_fullStr	Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia
title_full_unstemmed	Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia
title_sort	hydrocarbon degradation by bacteria from rhizospheres of imperata cylindrica at oil mining site in wonocolo, bojonegoro, indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/e181f83c0c914fdc9261ae5c87b1291a
work_keys_str_mv	AT mutiaraarumsari hydrocarbondegradationbybacteriafromrhizospheresofimperatacylindricaatoilminingsiteinwonocolobojonegoroindonesia AT endahretnaningrum hydrocarbondegradationbybacteriafromrhizospheresofimperatacylindricaatoilminingsiteinwonocolobojonegoroindonesia
_version_	1718418673756012544

Hydrocarbon degradation by bacteria from rhizospheres of Imperata cylindrica at oil mining site in Wonocolo, Bojonegoro, Indonesia

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