Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering

Sleeping Beauty is used as a genome engineering tool in a range of organisms. Here, the authors solve an atomic structure of Sleeping Beauty (SB) transposase and model the target DNA into the active site, elucidating details that may enable the rational design of novel transposases.

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Autores principales:	Franka Voigt, Lisa Wiedemann, Cecilia Zuliani, Irma Querques, Attila Sebe, Lajos Mátés, Zsuzsanna Izsvák, Zoltán Ivics, Orsolya Barabas
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e1827e65d71848d68d9652376597ea67
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spelling	oai:doaj.org-article:e1827e65d71848d68d9652376597ea672021-12-02T16:49:42ZSleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering10.1038/ncomms111262041-1723https://doaj.org/article/e1827e65d71848d68d9652376597ea672016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms11126https://doaj.org/toc/2041-1723Sleeping Beauty is used as a genome engineering tool in a range of organisms. Here, the authors solve an atomic structure of Sleeping Beauty (SB) transposase and model the target DNA into the active site, elucidating details that may enable the rational design of novel transposases.Franka VoigtLisa WiedemannCecilia ZulianiIrma QuerquesAttila SebeLajos MátésZsuzsanna IzsvákZoltán IvicsOrsolya BarabasNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-8 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Franka Voigt Lisa Wiedemann Cecilia Zuliani Irma Querques Attila Sebe Lajos Mátés Zsuzsanna Izsvák Zoltán Ivics Orsolya Barabas Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
description	Sleeping Beauty is used as a genome engineering tool in a range of organisms. Here, the authors solve an atomic structure of Sleeping Beauty (SB) transposase and model the target DNA into the active site, elucidating details that may enable the rational design of novel transposases.
format	article
author	Franka Voigt Lisa Wiedemann Cecilia Zuliani Irma Querques Attila Sebe Lajos Mátés Zsuzsanna Izsvák Zoltán Ivics Orsolya Barabas
author_facet	Franka Voigt Lisa Wiedemann Cecilia Zuliani Irma Querques Attila Sebe Lajos Mátés Zsuzsanna Izsvák Zoltán Ivics Orsolya Barabas
author_sort	Franka Voigt
title	Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
title_short	Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
title_full	Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
title_fullStr	Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
title_full_unstemmed	Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering
title_sort	sleeping beauty transposase structure allows rational design of hyperactive variants for genetic engineering
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/e1827e65d71848d68d9652376597ea67
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Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering

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