Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans

Background. Early detection of tick-borne pathogens in blood samples of infected people prevents the disease before clinical manifestations using antibiotic and immunoglobulin treatment. As far as tick-borne diseases are associated with significant activation of innate immune response, the changes i...

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Autores principales:	G. A. Danchinova, N. A. Lyapunova, E. L. Manzarova, I. S. Solovarov, I. V. Petrova, A. V. Lyapunov, J. Bataa, M. A. Khasnatinov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2018
Materias:	tick-borne infections tick-borne encephalitis lyme disease human monocytic ehrlichiosis human granulocytic anaplasmosis blood cytokines Science Q
Acceso en línea:	https://doaj.org/article/e185ffa03fb44a0c9f02271c6e46ca7c
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spelling	oai:doaj.org-article:e185ffa03fb44a0c9f02271c6e46ca7c2021-11-23T06:14:39ZPreliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans2541-94202587-959610.29413/ABS.2018-3.1.6https://doaj.org/article/e185ffa03fb44a0c9f02271c6e46ca7c2018-02-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/535https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Early detection of tick-borne pathogens in blood samples of infected people prevents the disease before clinical manifestations using antibiotic and immunoglobulin treatment. As far as tick-borne diseases are associated with significant activation of innate immune response, the changes in the cytokine profile soon after a tick bite could be used as an indicator of early tick-borne infection. Aims. The goal of this study was to characterize the production of cytokines during first 2-4 days after a tick bite in asymptomatic people infected with tick-borne pathogens. Materials and methods. The infection of tick-borne encephalitis virus, B. burgdorferi sensu lato, A. phagocytophilum and E. chaffeensis / E. muris was detected in blood of bitten humans using appropriate commercial real time RT-PCR and ELISA assays. Concentration of 14 cytokines in plasma of 89 infected and 45 non-infected people was established using quantitative ELISA kits. Results. Between 2nd and 4th days after tick bite, the prevalence and concentration of certain cytokines in blood of infected people significantly differed from those of the control group. Bacterial pathogens exhibited very similar patterns of cytokine profiles with induction of IL-1a, IL-8 и IFN-a and suppression of SOD, IL-1Ra и IL-17A. For virus infection the increased concentrations of IL-1 a, IL-8, IL-10, IL-17A, IFN-y and SOD were detected. The only suppressed cytokine was IL-1Ra receptor antagonist. Conclusions. The results suggest that evaluation of innate immune response between 2nd and 4th days after the tick bite could be a useful tool for evaluation of the risk of tick-borne disease for the humans bitten by ticks.G. A. DanchinovaN. A. LyapunovaE. L. ManzarovaI. S. SolovarovI. V. PetrovaA. V. LyapunovJ. BataaM. A. KhasnatinovScientific Сentre for Family Health and Human Reproduction Problemsarticletick-borne infectionstick-borne encephalitislyme diseasehuman monocytic ehrlichiosishuman granulocytic anaplasmosisbloodcytokinesScienceQRUActa Biomedica Scientifica, Vol 3, Iss 1, Pp 43-48 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	tick-borne infections tick-borne encephalitis lyme disease human monocytic ehrlichiosis human granulocytic anaplasmosis blood cytokines Science Q
spellingShingle	tick-borne infections tick-borne encephalitis lyme disease human monocytic ehrlichiosis human granulocytic anaplasmosis blood cytokines Science Q G. A. Danchinova N. A. Lyapunova E. L. Manzarova I. S. Solovarov I. V. Petrova A. V. Lyapunov J. Bataa M. A. Khasnatinov Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
description	Background. Early detection of tick-borne pathogens in blood samples of infected people prevents the disease before clinical manifestations using antibiotic and immunoglobulin treatment. As far as tick-borne diseases are associated with significant activation of innate immune response, the changes in the cytokine profile soon after a tick bite could be used as an indicator of early tick-borne infection. Aims. The goal of this study was to characterize the production of cytokines during first 2-4 days after a tick bite in asymptomatic people infected with tick-borne pathogens. Materials and methods. The infection of tick-borne encephalitis virus, B. burgdorferi sensu lato, A. phagocytophilum and E. chaffeensis / E. muris was detected in blood of bitten humans using appropriate commercial real time RT-PCR and ELISA assays. Concentration of 14 cytokines in plasma of 89 infected and 45 non-infected people was established using quantitative ELISA kits. Results. Between 2nd and 4th days after tick bite, the prevalence and concentration of certain cytokines in blood of infected people significantly differed from those of the control group. Bacterial pathogens exhibited very similar patterns of cytokine profiles with induction of IL-1a, IL-8 и IFN-a and suppression of SOD, IL-1Ra и IL-17A. For virus infection the increased concentrations of IL-1 a, IL-8, IL-10, IL-17A, IFN-y and SOD were detected. The only suppressed cytokine was IL-1Ra receptor antagonist. Conclusions. The results suggest that evaluation of innate immune response between 2nd and 4th days after the tick bite could be a useful tool for evaluation of the risk of tick-borne disease for the humans bitten by ticks.
format	article
author	G. A. Danchinova N. A. Lyapunova E. L. Manzarova I. S. Solovarov I. V. Petrova A. V. Lyapunov J. Bataa M. A. Khasnatinov
author_facet	G. A. Danchinova N. A. Lyapunova E. L. Manzarova I. S. Solovarov I. V. Petrova A. V. Lyapunov J. Bataa M. A. Khasnatinov
author_sort	G. A. Danchinova
title	Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
title_short	Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
title_full	Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
title_fullStr	Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
title_full_unstemmed	Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
title_sort	preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2018
url	https://doaj.org/article/e185ffa03fb44a0c9f02271c6e46ca7c
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Preliminary results of the study of subclinical infections of tick-borne pathogens in bitten humans

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