Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa

Abstract Iron is a limiting factor in such a condition that usually is sequestered by the host during polymicrobial infections of Pseudomonas aeruginosa and Staphylococcus aureus. This study aimed to investigate the interaction of S. aureus and P. aeruginosa, which alters iron-related sigma factors...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hamed Tahmasebi, Sanaz Dehbashi, Mohammad Reza Arabestani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e189ee8b45a746378673f4896c62aae3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e189ee8b45a746378673f4896c62aae3
record_format	dspace
spelling	oai:doaj.org-article:e189ee8b45a746378673f4896c62aae32021-12-02T18:02:31ZAntibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa10.1038/s41598-021-98017-52045-2322https://doaj.org/article/e189ee8b45a746378673f4896c62aae32021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98017-5https://doaj.org/toc/2045-2322Abstract Iron is a limiting factor in such a condition that usually is sequestered by the host during polymicrobial infections of Pseudomonas aeruginosa and Staphylococcus aureus. This study aimed to investigate the interaction of S. aureus and P. aeruginosa, which alters iron-related sigma factors regulation and antibiotic resistance. The antibiotic resistance of P. aeruginosa and S. aureus was investigated in a L929 cell culture model. The expression level of pvdS, hasI (P. aeruginosa sigma factors), and sigS (S. aureus sigma factor) genes was determined using Quantitative Real-Time PCR. pvdS and hasI were downregulated during co-culture with S. aureus, while the susceptibility to carbapenems increased (p-value < 0.0001). Also, there was a direct significant relationship between resistance to vancomycin with sigS. Regarding the findings of the current study, iron-related sigma factors of P. aeruginosa and S. aureus play a role in induction susceptibility to various antibiotics, including carbapenems and vancomycin.Hamed TahmasebiSanaz DehbashiMohammad Reza ArabestaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamed Tahmasebi Sanaz Dehbashi Mohammad Reza Arabestani Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa
description	Abstract Iron is a limiting factor in such a condition that usually is sequestered by the host during polymicrobial infections of Pseudomonas aeruginosa and Staphylococcus aureus. This study aimed to investigate the interaction of S. aureus and P. aeruginosa, which alters iron-related sigma factors regulation and antibiotic resistance. The antibiotic resistance of P. aeruginosa and S. aureus was investigated in a L929 cell culture model. The expression level of pvdS, hasI (P. aeruginosa sigma factors), and sigS (S. aureus sigma factor) genes was determined using Quantitative Real-Time PCR. pvdS and hasI were downregulated during co-culture with S. aureus, while the susceptibility to carbapenems increased (p-value < 0.0001). Also, there was a direct significant relationship between resistance to vancomycin with sigS. Regarding the findings of the current study, iron-related sigma factors of P. aeruginosa and S. aureus play a role in induction susceptibility to various antibiotics, including carbapenems and vancomycin.
format	article
author	Hamed Tahmasebi Sanaz Dehbashi Mohammad Reza Arabestani
author_facet	Hamed Tahmasebi Sanaz Dehbashi Mohammad Reza Arabestani
author_sort	Hamed Tahmasebi
title	Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa
title_short	Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa
title_full	Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa
title_fullStr	Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa
title_full_unstemmed	Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa
title_sort	antibiotic resistance alters through iron-regulating sigma factors during the interaction of staphylococcus aureus and pseudomonas aeruginosa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e189ee8b45a746378673f4896c62aae3
work_keys_str_mv	AT hamedtahmasebi antibioticresistancealtersthroughironregulatingsigmafactorsduringtheinteractionofstaphylococcusaureusandpseudomonasaeruginosa AT sanazdehbashi antibioticresistancealtersthroughironregulatingsigmafactorsduringtheinteractionofstaphylococcusaureusandpseudomonasaeruginosa AT mohammadrezaarabestani antibioticresistancealtersthroughironregulatingsigmafactorsduringtheinteractionofstaphylococcusaureusandpseudomonasaeruginosa
_version_	1718378899730071552

Antibiotic resistance alters through iron-regulating Sigma factors during the interaction of Staphylococcus aureus and Pseudomonas aeruginosa

Ejemplares similares