Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions

Abstract Juxtacellular interactions play an essential but still not fully understood role in both normal tissue development and tumour invasion. Using proliferating cell fronts as a model system, we explore the effects of cell–cell interactions on the geometry and dynamics of these one-dimensional b...

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Main Authors:	Guillaume Rapin, Nirvana Caballero, Iaroslav Gaponenko, Benedikt Ziegler, Audrey Rawleigh, Ermanno Moriggi, Thierry Giamarchi, Steven A. Brown, Patrycja Paruch
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Medicine R Science Q
Online Access:	https://doaj.org/article/e18f8b8ed0934d0dab4a9cb28285b70b
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spelling	oai:doaj.org-article:e18f8b8ed0934d0dab4a9cb28285b70b2021-12-02T17:32:58ZRoughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions10.1038/s41598-021-86684-32045-2322https://doaj.org/article/e18f8b8ed0934d0dab4a9cb28285b70b2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86684-3https://doaj.org/toc/2045-2322Abstract Juxtacellular interactions play an essential but still not fully understood role in both normal tissue development and tumour invasion. Using proliferating cell fronts as a model system, we explore the effects of cell–cell interactions on the geometry and dynamics of these one-dimensional biological interfaces. We observe two distinct scaling regimes of the steady state roughness of in-vitro propagating Rat1 fibroblast cell fronts, suggesting different hierarchies of interactions at sub-cell lengthscales and at a lengthscale of 2–10 cells. Pharmacological modulation significantly affects the proliferation speed of the cell fronts, and those modulators that promote cell mobility or division also lead to the most rapid evolution of cell front roughness. By comparing our experimental observations to numerical simulations of elastic cell fronts with purely short-range interactions, we demonstrate that the interactions at few-cell lengthscales play a key role. Our methodology provides a simple framework to measure and characterise the biological effects of such interactions, and could be useful in tumour phenotyping.Guillaume RapinNirvana CaballeroIaroslav GaponenkoBenedikt ZieglerAudrey RawleighErmanno MoriggiThierry GiamarchiSteven A. BrownPatrycja ParuchNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Guillaume Rapin Nirvana Caballero Iaroslav Gaponenko Benedikt Ziegler Audrey Rawleigh Ermanno Moriggi Thierry Giamarchi Steven A. Brown Patrycja Paruch Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
description	Abstract Juxtacellular interactions play an essential but still not fully understood role in both normal tissue development and tumour invasion. Using proliferating cell fronts as a model system, we explore the effects of cell–cell interactions on the geometry and dynamics of these one-dimensional biological interfaces. We observe two distinct scaling regimes of the steady state roughness of in-vitro propagating Rat1 fibroblast cell fronts, suggesting different hierarchies of interactions at sub-cell lengthscales and at a lengthscale of 2–10 cells. Pharmacological modulation significantly affects the proliferation speed of the cell fronts, and those modulators that promote cell mobility or division also lead to the most rapid evolution of cell front roughness. By comparing our experimental observations to numerical simulations of elastic cell fronts with purely short-range interactions, we demonstrate that the interactions at few-cell lengthscales play a key role. Our methodology provides a simple framework to measure and characterise the biological effects of such interactions, and could be useful in tumour phenotyping.
format	article
author	Guillaume Rapin Nirvana Caballero Iaroslav Gaponenko Benedikt Ziegler Audrey Rawleigh Ermanno Moriggi Thierry Giamarchi Steven A. Brown Patrycja Paruch
author_facet	Guillaume Rapin Nirvana Caballero Iaroslav Gaponenko Benedikt Ziegler Audrey Rawleigh Ermanno Moriggi Thierry Giamarchi Steven A. Brown Patrycja Paruch
author_sort	Guillaume Rapin
title	Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
title_short	Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
title_full	Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
title_fullStr	Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
title_full_unstemmed	Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
title_sort	roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e18f8b8ed0934d0dab4a9cb28285b70b
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Roughness and dynamics of proliferating cell fronts as a probe of cell–cell interactions

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