Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities

Abstract Interferons (IFNs) are essential components of the host innate immune system and define first-line of defence against pathogens. In mammals, several type I IFNs are identified, however, only limited data is available on the repertoire of IFNs in avian species. Here we report the characteriz...

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Autores principales:	Diwakar Santhakumar, Munir Iqbal, Venugopal Nair, Muhammad Munir
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e19477b7e501478aa9e819f55c893735
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spelling	oai:doaj.org-article:e19477b7e501478aa9e819f55c8937352021-12-02T12:30:51ZChicken IFN Kappa: A Novel Cytokine with Antiviral Activities10.1038/s41598-017-02951-22045-2322https://doaj.org/article/e19477b7e501478aa9e819f55c8937352017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02951-2https://doaj.org/toc/2045-2322Abstract Interferons (IFNs) are essential components of the host innate immune system and define first-line of defence against pathogens. In mammals, several type I IFNs are identified, however, only limited data is available on the repertoire of IFNs in avian species. Here we report the characterization of chicken IFN-κ (chIFN-κ) near the type I IFN locus on the sex-determining Z chromosome. Genetic, evolutionary and syntenic analyses indicate that chIFN-κ is a type I IFN with conserved genetic features and promoter binding sites. chIFN-κ regulated the IFN-stimulated response element signalling pathways and activated a panel of IFN-regulated genes, antiviral mediators and transcriptional regulators. Priming of chicken primary fibroblasts and tracheal organ cultures with chIFN-κ imparted cellular protections against viral infections both in vitro and ex vivo. To determine whether chIFN-κ defines the antiviral state in developing chicken embryos, we used replication-competent retroviral RCAS vector system to generate transgenic chicken embryos that constitutively and stably expressed chIFN-κ. We could demonstrate that chIFN-κ markedly inhibited the replication of avian RNA viruses in ovo. Collectively, these results shed the light on the repertoire of IFNs in avian species and provide functional data on the interaction of the chIFN-κ with RNA viruses of poultry and public health importance.Diwakar SanthakumarMunir IqbalVenugopal NairMuhammad MunirNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Diwakar Santhakumar Munir Iqbal Venugopal Nair Muhammad Munir Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
description	Abstract Interferons (IFNs) are essential components of the host innate immune system and define first-line of defence against pathogens. In mammals, several type I IFNs are identified, however, only limited data is available on the repertoire of IFNs in avian species. Here we report the characterization of chicken IFN-κ (chIFN-κ) near the type I IFN locus on the sex-determining Z chromosome. Genetic, evolutionary and syntenic analyses indicate that chIFN-κ is a type I IFN with conserved genetic features and promoter binding sites. chIFN-κ regulated the IFN-stimulated response element signalling pathways and activated a panel of IFN-regulated genes, antiviral mediators and transcriptional regulators. Priming of chicken primary fibroblasts and tracheal organ cultures with chIFN-κ imparted cellular protections against viral infections both in vitro and ex vivo. To determine whether chIFN-κ defines the antiviral state in developing chicken embryos, we used replication-competent retroviral RCAS vector system to generate transgenic chicken embryos that constitutively and stably expressed chIFN-κ. We could demonstrate that chIFN-κ markedly inhibited the replication of avian RNA viruses in ovo. Collectively, these results shed the light on the repertoire of IFNs in avian species and provide functional data on the interaction of the chIFN-κ with RNA viruses of poultry and public health importance.
format	article
author	Diwakar Santhakumar Munir Iqbal Venugopal Nair Muhammad Munir
author_facet	Diwakar Santhakumar Munir Iqbal Venugopal Nair Muhammad Munir
author_sort	Diwakar Santhakumar
title	Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
title_short	Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
title_full	Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
title_fullStr	Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
title_full_unstemmed	Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities
title_sort	chicken ifn kappa: a novel cytokine with antiviral activities
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e19477b7e501478aa9e819f55c893735
work_keys_str_mv	AT diwakarsanthakumar chickenifnkappaanovelcytokinewithantiviralactivities AT muniriqbal chickenifnkappaanovelcytokinewithantiviralactivities AT venugopalnair chickenifnkappaanovelcytokinewithantiviralactivities AT muhammadmunir chickenifnkappaanovelcytokinewithantiviralactivities
_version_	1718394310608551936

Chicken IFN Kappa: A Novel Cytokine with Antiviral Activities

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