Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.

Cyclooxygenase-2 (COX-2) expression has been detected in human hepatoma cell lines and in human hepatocellular carcinoma (HCC); however, the contribution of COX-2 to the development of HCC remains controversial. COX-2 expression is higher in the non-tumoral tissue and inversely correlates with the d...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Noelia Agra Andrieu, Omar Motiño, Rafael Mayoral, Cristina Llorente Izquierdo, Ana Fernández-Alvarez, Lisardo Boscá, Marta Casado, Paloma Martín-Sanz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e19f93e6afc54af6a5910cebb21ed688
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e19f93e6afc54af6a5910cebb21ed688
record_format	dspace
spelling	oai:doaj.org-article:e19f93e6afc54af6a5910cebb21ed6882021-11-18T08:06:38ZCyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.1932-620310.1371/journal.pone.0050935https://doaj.org/article/e19f93e6afc54af6a5910cebb21ed6882012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23226427/?tool=EBIhttps://doaj.org/toc/1932-6203Cyclooxygenase-2 (COX-2) expression has been detected in human hepatoma cell lines and in human hepatocellular carcinoma (HCC); however, the contribution of COX-2 to the development of HCC remains controversial. COX-2 expression is higher in the non-tumoral tissue and inversely correlates with the differentiation grade of the tumor. COX-2 expression depends on the interplay between different cellular pathways involving both transcriptional and post-transcriptional regulation. The aim of this work was to assess whether COX-2 could be regulated by microRNAs in human hepatoma cell lines and in human HCC specimens since these molecules contribute to the regulation of genes implicated in cell growth and differentiation. Our results show that miR-16 silences COX-2 expression in hepatoma cells by two mechanisms: a) by binding directly to the microRNA response element (MRE) in the COX-2 3'-UTR promoting translational suppression of COX-2 mRNA; b) by decreasing the levels of the RNA-binding protein Human Antigen R (HuR). Furthermore, ectopic expression of miR-16 inhibits cell proliferation, promotes cell apoptosis and suppresses the ability of hepatoma cells to develop tumors in nude mice, partially through targeting COX-2. Moreover a reduced miR-16 expression tends to correlate to high levels of COX-2 protein in liver from patients affected by HCC. Our data show an important role for miR-16 as a post-transcriptional regulator of COX-2 in HCC and suggest the potential therapeutic application of miR-16 in those HCC with a high COX-2 expression.Noelia Agra AndrieuOmar MotiñoRafael MayoralCristina Llorente IzquierdoAna Fernández-AlvarezLisardo BoscáMarta CasadoPaloma Martín-SanzPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50935 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Noelia Agra Andrieu Omar Motiño Rafael Mayoral Cristina Llorente Izquierdo Ana Fernández-Alvarez Lisardo Boscá Marta Casado Paloma Martín-Sanz Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.
description	Cyclooxygenase-2 (COX-2) expression has been detected in human hepatoma cell lines and in human hepatocellular carcinoma (HCC); however, the contribution of COX-2 to the development of HCC remains controversial. COX-2 expression is higher in the non-tumoral tissue and inversely correlates with the differentiation grade of the tumor. COX-2 expression depends on the interplay between different cellular pathways involving both transcriptional and post-transcriptional regulation. The aim of this work was to assess whether COX-2 could be regulated by microRNAs in human hepatoma cell lines and in human HCC specimens since these molecules contribute to the regulation of genes implicated in cell growth and differentiation. Our results show that miR-16 silences COX-2 expression in hepatoma cells by two mechanisms: a) by binding directly to the microRNA response element (MRE) in the COX-2 3'-UTR promoting translational suppression of COX-2 mRNA; b) by decreasing the levels of the RNA-binding protein Human Antigen R (HuR). Furthermore, ectopic expression of miR-16 inhibits cell proliferation, promotes cell apoptosis and suppresses the ability of hepatoma cells to develop tumors in nude mice, partially through targeting COX-2. Moreover a reduced miR-16 expression tends to correlate to high levels of COX-2 protein in liver from patients affected by HCC. Our data show an important role for miR-16 as a post-transcriptional regulator of COX-2 in HCC and suggest the potential therapeutic application of miR-16 in those HCC with a high COX-2 expression.
format	article
author	Noelia Agra Andrieu Omar Motiño Rafael Mayoral Cristina Llorente Izquierdo Ana Fernández-Alvarez Lisardo Boscá Marta Casado Paloma Martín-Sanz
author_facet	Noelia Agra Andrieu Omar Motiño Rafael Mayoral Cristina Llorente Izquierdo Ana Fernández-Alvarez Lisardo Boscá Marta Casado Paloma Martín-Sanz
author_sort	Noelia Agra Andrieu
title	Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.
title_short	Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.
title_full	Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.
title_fullStr	Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.
title_full_unstemmed	Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.
title_sort	cyclooxygenase-2 is a target of microrna-16 in human hepatoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e19f93e6afc54af6a5910cebb21ed688
work_keys_str_mv	AT noeliaagraandrieu cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT omarmotino cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT rafaelmayoral cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT cristinallorenteizquierdo cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT anafernandezalvarez cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT lisardobosca cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT martacasado cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells AT palomamartinsanz cyclooxygenase2isatargetofmicrorna16inhumanhepatomacells
_version_	1718422262495838208

Cyclooxygenase-2 is a target of microRNA-16 in human hepatoma cells.

Ejemplares similares