A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge

Abstract Schmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleopro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hani Boshra, Gema Lorenzo, Diego Charro, Sandra Moreno, Gabriel Soares Guerra, Isbene Sanchez, Joseba M. Garrido, Marivi Geijo, Alejandro Brun, Nicola G. A. Abrescia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1a0a7a06f3441369256cd76cabebf70
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e1a0a7a06f3441369256cd76cabebf70
record_format	dspace
spelling	oai:doaj.org-article:e1a0a7a06f3441369256cd76cabebf702021-12-02T11:41:56ZA novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge10.1038/s41598-020-73424-22045-2322https://doaj.org/article/e1a0a7a06f3441369256cd76cabebf702020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-73424-2https://doaj.org/toc/2045-2322Abstract Schmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleoprotein (SBV-N) administered with a veterinary-grade Saponin adjuvant. When assayed in an IFNAR-/- mouse model, SBV-N with Saponin induced strong non-neutralizing broadly virus-reactive antibodies, decreased clinical signs, as well as significantly reduced viremia. Vaccination assays also suggest that this level of immune protection is cell mediated, as evidenced by the lack of neutralizing antibodies, as well as interferon-γ secretion observed in vitro. Therefore, based on these results, bacterially expressed SBV-N, co-administered with veterinary-grade Saponin adjuvant may serve as a promising economical alternative to current SBV vaccines, and warrant further evaluation in large ruminant animal models. Moreover, we propose that this strategy may be applicable to other bunyaviruses.Hani BoshraGema LorenzoDiego CharroSandra MorenoGabriel Soares GuerraIsbene SanchezJoseba M. GarridoMarivi GeijoAlejandro BrunNicola G. A. AbresciaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hani Boshra Gema Lorenzo Diego Charro Sandra Moreno Gabriel Soares Guerra Isbene Sanchez Joseba M. Garrido Marivi Geijo Alejandro Brun Nicola G. A. Abrescia A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge
description	Abstract Schmallenberg virus (SBV), an arthropod-transmitted pathogenic bunyavirus, continues to be a threat to the European livestock industry, causing morbidity and mortality among young ruminant livestock. Here, we describe a novel SBV subunit vaccine, based on bacterially expressed SBV nucleoprotein (SBV-N) administered with a veterinary-grade Saponin adjuvant. When assayed in an IFNAR-/- mouse model, SBV-N with Saponin induced strong non-neutralizing broadly virus-reactive antibodies, decreased clinical signs, as well as significantly reduced viremia. Vaccination assays also suggest that this level of immune protection is cell mediated, as evidenced by the lack of neutralizing antibodies, as well as interferon-γ secretion observed in vitro. Therefore, based on these results, bacterially expressed SBV-N, co-administered with veterinary-grade Saponin adjuvant may serve as a promising economical alternative to current SBV vaccines, and warrant further evaluation in large ruminant animal models. Moreover, we propose that this strategy may be applicable to other bunyaviruses.
format	article
author	Hani Boshra Gema Lorenzo Diego Charro Sandra Moreno Gabriel Soares Guerra Isbene Sanchez Joseba M. Garrido Marivi Geijo Alejandro Brun Nicola G. A. Abrescia
author_facet	Hani Boshra Gema Lorenzo Diego Charro Sandra Moreno Gabriel Soares Guerra Isbene Sanchez Joseba M. Garrido Marivi Geijo Alejandro Brun Nicola G. A. Abrescia
author_sort	Hani Boshra
title	A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge
title_short	A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge
title_full	A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge
title_fullStr	A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge
title_full_unstemmed	A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge
title_sort	novel schmallenberg virus subunit vaccine candidate protects ifnar-/- mice against virulent sbv challenge
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e1a0a7a06f3441369256cd76cabebf70
work_keys_str_mv	AT haniboshra anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT gemalorenzo anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT diegocharro anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT sandramoreno anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT gabrielsoaresguerra anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT isbenesanchez anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT josebamgarrido anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT marivigeijo anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT alejandrobrun anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT nicolagaabrescia anovelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT haniboshra novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT gemalorenzo novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT diegocharro novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT sandramoreno novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT gabrielsoaresguerra novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT isbenesanchez novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT josebamgarrido novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT marivigeijo novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT alejandrobrun novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge AT nicolagaabrescia novelschmallenbergvirussubunitvaccinecandidateprotectsifnarmiceagainstvirulentsbvchallenge
_version_	1718395374227423232

A novel Schmallenberg virus subunit vaccine candidate protects IFNAR-/- mice against virulent SBV challenge

Ejemplares similares