Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria

Abstract Incomplete non-sterile immunity to malaria is attained in endemic regions after recurrent infections by a large percentage of the adult population, who carry the malaria parasite asymptomatically. Although blood-stage Plasmodium falciparum rapidly elicits IgG responses, the target antigens...

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Autores principales:	Isabel G. Azcárate, Patricia Marín-García, Paloma Abad, Susana Pérez-Benavente, Estela Paz-Artal, Pedro A. Reche, Julius N. Fobil, José M. Rubio, Amalia Diez, Antonio Puyet, José M. Bautista
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1b13b9f63974146b44fcff667c009d8
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spelling	oai:doaj.org-article:e1b13b9f63974146b44fcff667c009d82021-12-02T17:52:33ZPlasmodium falciparum immunodominant IgG epitopes in subclinical malaria10.1038/s41598-020-66384-02045-2322https://doaj.org/article/e1b13b9f63974146b44fcff667c009d82020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66384-0https://doaj.org/toc/2045-2322Abstract Incomplete non-sterile immunity to malaria is attained in endemic regions after recurrent infections by a large percentage of the adult population, who carry the malaria parasite asymptomatically. Although blood-stage Plasmodium falciparum rapidly elicits IgG responses, the target antigens of partially protective and non-protective IgG antibodies as well as the basis for the acquisition of these antibodies remain largely unknown. We performed IgG-immunomics to screen for P. falciparum antigens and to identify epitopes associated with exposure and clinical disease. Sera from malaria cases identified five prevalent antigens recognized by all analyzed patients’ IgGs. Epitope mapping of them, using adult and children sera samples from an endemic malaria region in Ghana segregated into patients with positive or negative subclinical detection of P. falciparum, revealed binding specificity for two 20-mer immunodominant antigenic regions within the START-related lipid transfer protein and the protein disulfide isomerase PDI8. These 20-mer epitopes challenged with sera samples from children under 5 years old displayed specific IgG binding in those with detectable parasitemia, even at subclinical level. These results suggest that humoral response against START and PDI8 antigens may be triggered at submicroscopic parasitemia levels in children and may eventually be used to differentially diagnose subclinical malaria in children.Isabel G. AzcáratePatricia Marín-GarcíaPaloma AbadSusana Pérez-BenaventeEstela Paz-ArtalPedro A. RecheJulius N. FobilJosé M. RubioAmalia DiezAntonio PuyetJosé M. BautistaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Isabel G. Azcárate Patricia Marín-García Paloma Abad Susana Pérez-Benavente Estela Paz-Artal Pedro A. Reche Julius N. Fobil José M. Rubio Amalia Diez Antonio Puyet José M. Bautista Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
description	Abstract Incomplete non-sterile immunity to malaria is attained in endemic regions after recurrent infections by a large percentage of the adult population, who carry the malaria parasite asymptomatically. Although blood-stage Plasmodium falciparum rapidly elicits IgG responses, the target antigens of partially protective and non-protective IgG antibodies as well as the basis for the acquisition of these antibodies remain largely unknown. We performed IgG-immunomics to screen for P. falciparum antigens and to identify epitopes associated with exposure and clinical disease. Sera from malaria cases identified five prevalent antigens recognized by all analyzed patients’ IgGs. Epitope mapping of them, using adult and children sera samples from an endemic malaria region in Ghana segregated into patients with positive or negative subclinical detection of P. falciparum, revealed binding specificity for two 20-mer immunodominant antigenic regions within the START-related lipid transfer protein and the protein disulfide isomerase PDI8. These 20-mer epitopes challenged with sera samples from children under 5 years old displayed specific IgG binding in those with detectable parasitemia, even at subclinical level. These results suggest that humoral response against START and PDI8 antigens may be triggered at submicroscopic parasitemia levels in children and may eventually be used to differentially diagnose subclinical malaria in children.
format	article
author	Isabel G. Azcárate Patricia Marín-García Paloma Abad Susana Pérez-Benavente Estela Paz-Artal Pedro A. Reche Julius N. Fobil José M. Rubio Amalia Diez Antonio Puyet José M. Bautista
author_facet	Isabel G. Azcárate Patricia Marín-García Paloma Abad Susana Pérez-Benavente Estela Paz-Artal Pedro A. Reche Julius N. Fobil José M. Rubio Amalia Diez Antonio Puyet José M. Bautista
author_sort	Isabel G. Azcárate
title	Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
title_short	Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
title_full	Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
title_fullStr	Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
title_full_unstemmed	Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria
title_sort	plasmodium falciparum immunodominant igg epitopes in subclinical malaria
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e1b13b9f63974146b44fcff667c009d8
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Plasmodium falciparum immunodominant IgG epitopes in subclinical malaria

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