Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.

Conventional vaccines to prevent the pneumonia caused by Rhodococcus equi have not been successful. We have recently demonstrated that immunization with Salmonella enterica Typhimurium expressing the VapA antigen protects mice against R. equi infection. We now report that oral vaccination of mice wi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aline F Oliveira, Luciana P Ruas, Silvia A Cardoso, Sandro G Soares, Maria-Cristina Roque-Barreira
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1bb7c85ea274d8c91e2092867a69e81
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e1bb7c85ea274d8c91e2092867a69e81
record_format	dspace
spelling	oai:doaj.org-article:e1bb7c85ea274d8c91e2092867a69e812021-11-25T06:26:48ZVaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.1932-620310.1371/journal.pone.0008644https://doaj.org/article/e1bb7c85ea274d8c91e2092867a69e812010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20072623/?tool=EBIhttps://doaj.org/toc/1932-6203Conventional vaccines to prevent the pneumonia caused by Rhodococcus equi have not been successful. We have recently demonstrated that immunization with Salmonella enterica Typhimurium expressing the VapA antigen protects mice against R. equi infection. We now report that oral vaccination of mice with this recombinant strain results in high and persistent fecal levels of antigen-specific IgA, and specific proliferation of the spleen cells of immunized mice in response to the in vitro stimulation with R. equi antigen. After in vitro stimulation, spleen cells of immunized mice produce high levels of Th1 cytokines and show a prominent mRNA expression of the Th1 transcription factor T-bet, in detriment of the Th2 transcription factor GATA-3. Following R. equi challenge, a high H2O2, NO, IL-12, and IFN-gamma content is detected in the organs of immunized mice. On the other hand, TNF-alpha and IL-4 levels are markedly lower in the organs of vaccinated mice, compared with the non-vaccinated ones. The IL-10 content and the mRNA transcription level of TGF-beta are also higher in the organs of immunized mice. A greater incidence of CD4+ and CD8+ T cells and B lymphocytes is verified in vaccinated mice. However, there is no difference between vaccinated and non-vaccinated mice in terms of the frequency of CD4+CD25+Foxp3+ T cells. Finally, we show that the vaccination confers a long-term protection against R. equi infection. Altogether, these data indicate that the oral vaccination of mice with S. enterica Typhimurium expressing VapA induces specific and long-lasting humoral and cellular responses against the pathogen, which are appropriately regulated and allow tissue integrity after challenge.Aline F OliveiraLuciana P RuasSilvia A CardosoSandro G SoaresMaria-Cristina Roque-BarreiraPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8644 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aline F Oliveira Luciana P Ruas Silvia A Cardoso Sandro G Soares Maria-Cristina Roque-Barreira Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.
description	Conventional vaccines to prevent the pneumonia caused by Rhodococcus equi have not been successful. We have recently demonstrated that immunization with Salmonella enterica Typhimurium expressing the VapA antigen protects mice against R. equi infection. We now report that oral vaccination of mice with this recombinant strain results in high and persistent fecal levels of antigen-specific IgA, and specific proliferation of the spleen cells of immunized mice in response to the in vitro stimulation with R. equi antigen. After in vitro stimulation, spleen cells of immunized mice produce high levels of Th1 cytokines and show a prominent mRNA expression of the Th1 transcription factor T-bet, in detriment of the Th2 transcription factor GATA-3. Following R. equi challenge, a high H2O2, NO, IL-12, and IFN-gamma content is detected in the organs of immunized mice. On the other hand, TNF-alpha and IL-4 levels are markedly lower in the organs of vaccinated mice, compared with the non-vaccinated ones. The IL-10 content and the mRNA transcription level of TGF-beta are also higher in the organs of immunized mice. A greater incidence of CD4+ and CD8+ T cells and B lymphocytes is verified in vaccinated mice. However, there is no difference between vaccinated and non-vaccinated mice in terms of the frequency of CD4+CD25+Foxp3+ T cells. Finally, we show that the vaccination confers a long-term protection against R. equi infection. Altogether, these data indicate that the oral vaccination of mice with S. enterica Typhimurium expressing VapA induces specific and long-lasting humoral and cellular responses against the pathogen, which are appropriately regulated and allow tissue integrity after challenge.
format	article
author	Aline F Oliveira Luciana P Ruas Silvia A Cardoso Sandro G Soares Maria-Cristina Roque-Barreira
author_facet	Aline F Oliveira Luciana P Ruas Silvia A Cardoso Sandro G Soares Maria-Cristina Roque-Barreira
author_sort	Aline F Oliveira
title	Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.
title_short	Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.
title_full	Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.
title_fullStr	Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.
title_full_unstemmed	Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.
title_sort	vaccination of mice with salmonella expressing vapa: mucosal and systemic th1 responses provide protection against rhodococcus equi infection.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e1bb7c85ea274d8c91e2092867a69e81
work_keys_str_mv	AT alinefoliveira vaccinationofmicewithsalmonellaexpressingvapamucosalandsystemicth1responsesprovideprotectionagainstrhodococcusequiinfection AT lucianapruas vaccinationofmicewithsalmonellaexpressingvapamucosalandsystemicth1responsesprovideprotectionagainstrhodococcusequiinfection AT silviaacardoso vaccinationofmicewithsalmonellaexpressingvapamucosalandsystemicth1responsesprovideprotectionagainstrhodococcusequiinfection AT sandrogsoares vaccinationofmicewithsalmonellaexpressingvapamucosalandsystemicth1responsesprovideprotectionagainstrhodococcusequiinfection AT mariacristinaroquebarreira vaccinationofmicewithsalmonellaexpressingvapamucosalandsystemicth1responsesprovideprotectionagainstrhodococcusequiinfection
_version_	1718413714356436992

Vaccination of mice with salmonella expressing VapA: mucosal and systemic Th1 responses provide protection against Rhodococcus equi infection.

Ejemplares similares