Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures

Atsushi Yoshino,1 Natalia Polouliakh,1–3 Akira Meguro,1 Masaki Takeuchi,1,4 Tatsukata Kawagoe,1 Nobuhisa Mizuki1 1Department of Ophthalmology and Visual Science, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, 2Sony Computer Science Laboratories Inc., Fundamental...

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Autores principales:	Yoshino A, Polouliakh N, Meguro A, Takeuchi M, Kawagoe T, Mizuki N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	fish egg antiaging gene expression analysis antioxidative gene phylogenetic footprinting analysis Geriatrics RC952-954.6
Acceso en línea:	https://doaj.org/article/e1bf5ff4b9c744f9be72558f6ea512b3
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spelling	oai:doaj.org-article:e1bf5ff4b9c744f9be72558f6ea512b32021-12-02T09:03:52ZChum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures1178-1998https://doaj.org/article/e1bf5ff4b9c744f9be72558f6ea512b32016-08-01T00:00:00Zhttps://www.dovepress.com/chum-salmon-egg-extracts-induce-upregulation-of-collagen-type-i-and-ex-peer-reviewed-article-CIAhttps://doaj.org/toc/1178-1998Atsushi Yoshino,1 Natalia Polouliakh,1–3 Akira Meguro,1 Masaki Takeuchi,1,4 Tatsukata Kawagoe,1 Nobuhisa Mizuki1 1Department of Ophthalmology and Visual Science, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, 2Sony Computer Science Laboratories Inc., Fundamental Research Laboratories, 3Systems Biology Institute, Tokyo, Japan; 4Inflammatory Disease Section, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA Abstract: Components of fish roe possess antioxidant and antiaging activities, making them potentially very beneficial natural resources. Here, we investigated chum salmon eggs (CSEs) as a source of active ingredients, including vitamins, unsaturated fatty acids, and proteins. We incubated human dermal fibroblast cultures for 48 hours with high and low concentrations of CSE extracts and analyzed changes in gene expression. Cells treated with CSE extract showed concentration-dependent upregulation of collagen type I genes and of multiple antioxidative genes, including OXR1, TXNRD1, and PRDX family genes. We further conducted in silico phylogenetic footprinting analysis of promoter regions. These results suggested that transcription factors such as acute myeloid leukemia-1a and cyclic adenosine monophosphate response element-binding protein may be involved in the observed upregulation of antioxidative genes. Our results support the idea that CSEs are strong candidate sources of antioxidant materials and cosmeceutically effective ingredients. Keywords: fish egg, antiaging, gene expression analysis, antioxidative gene, phylogenetic footprinting analysisYoshino APolouliakh NMeguro ATakeuchi MKawagoe TMizuki NDove Medical Pressarticlefish eggantiaginggene expression analysisantioxidative genephylogenetic footprinting analysisGeriatricsRC952-954.6ENClinical Interventions in Aging, Vol Volume 11, Pp 1159-1168 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	fish egg antiaging gene expression analysis antioxidative gene phylogenetic footprinting analysis Geriatrics RC952-954.6
spellingShingle	fish egg antiaging gene expression analysis antioxidative gene phylogenetic footprinting analysis Geriatrics RC952-954.6 Yoshino A Polouliakh N Meguro A Takeuchi M Kawagoe T Mizuki N Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures
description	Atsushi Yoshino,1 Natalia Polouliakh,1–3 Akira Meguro,1 Masaki Takeuchi,1,4 Tatsukata Kawagoe,1 Nobuhisa Mizuki1 1Department of Ophthalmology and Visual Science, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa, 2Sony Computer Science Laboratories Inc., Fundamental Research Laboratories, 3Systems Biology Institute, Tokyo, Japan; 4Inflammatory Disease Section, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA Abstract: Components of fish roe possess antioxidant and antiaging activities, making them potentially very beneficial natural resources. Here, we investigated chum salmon eggs (CSEs) as a source of active ingredients, including vitamins, unsaturated fatty acids, and proteins. We incubated human dermal fibroblast cultures for 48 hours with high and low concentrations of CSE extracts and analyzed changes in gene expression. Cells treated with CSE extract showed concentration-dependent upregulation of collagen type I genes and of multiple antioxidative genes, including OXR1, TXNRD1, and PRDX family genes. We further conducted in silico phylogenetic footprinting analysis of promoter regions. These results suggested that transcription factors such as acute myeloid leukemia-1a and cyclic adenosine monophosphate response element-binding protein may be involved in the observed upregulation of antioxidative genes. Our results support the idea that CSEs are strong candidate sources of antioxidant materials and cosmeceutically effective ingredients. Keywords: fish egg, antiaging, gene expression analysis, antioxidative gene, phylogenetic footprinting analysis
format	article
author	Yoshino A Polouliakh N Meguro A Takeuchi M Kawagoe T Mizuki N
author_facet	Yoshino A Polouliakh N Meguro A Takeuchi M Kawagoe T Mizuki N
author_sort	Yoshino A
title	Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures
title_short	Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures
title_full	Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures
title_fullStr	Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures
title_full_unstemmed	Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures
title_sort	chum salmon egg extracts induce upregulation of collagen type i and exert antioxidative effects on human dermal fibroblast cultures
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/e1bf5ff4b9c744f9be72558f6ea512b3
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_version_	1718398276005265408

Chum salmon egg extracts induce upregulation of collagen type I and exert antioxidative effects on human dermal fibroblast cultures

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