Metatranscriptomics of the Human Oral Microbiome during Health and Disease

ABSTRACT The human microbiome plays important roles in health, but when disrupted, these same indigenous microbes can cause disease. The composition of the microbiome changes during the transition from health to disease; however, these changes are often not conserved among patients. Since microbiome...

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Autores principales:	Peter Jorth, Keith H. Turner, Pinar Gumus, Nejat Nizam, Nurcan Buduneli, Marvin Whiteley
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e1c6317a50144420a8414a6ccd9e6781
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spelling	oai:doaj.org-article:e1c6317a50144420a8414a6ccd9e67812021-11-15T15:45:13ZMetatranscriptomics of the Human Oral Microbiome during Health and Disease10.1128/mBio.01012-142150-7511https://doaj.org/article/e1c6317a50144420a8414a6ccd9e67812014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01012-14https://doaj.org/toc/2150-7511ABSTRACT The human microbiome plays important roles in health, but when disrupted, these same indigenous microbes can cause disease. The composition of the microbiome changes during the transition from health to disease; however, these changes are often not conserved among patients. Since microbiome-associated diseases like periodontitis cause similar patient symptoms despite interpatient variability in microbial community composition, we hypothesized that human-associated microbial communities undergo conserved changes in metabolism during disease. Here, we used patient-matched healthy and diseased samples to compare gene expression of 160,000 genes in healthy and diseased periodontal communities. We show that health- and disease-associated communities exhibit defined differences in metabolism that are conserved between patients. In contrast, the metabolic gene expression of individual species was highly variable between patients. These results demonstrate that despite high interpatient variability in microbial composition, disease-associated communities display conserved metabolic profiles that are generally accomplished by a patient-specific cohort of microbes. IMPORTANCE The human microbiome project has shown that shifts in our microbiota are associated with many diseases, including obesity, Crohn’s disease, diabetes, and periodontitis. While changes in microbial populations are apparent during these diseases, the species associated with each disease can vary from patient to patient. Taking into account this interpatient variability, we hypothesized that specific microbiota-associated diseases would be marked by conserved microbial community behaviors. Here, we use gene expression analyses of patient-matched healthy and diseased human periodontal plaque to show that microbial communities have highly conserved metabolic gene expression profiles, whereas individual species within the community do not. Furthermore, disease-associated communities exhibit conserved changes in metabolic and virulence gene expression.Peter JorthKeith H. TurnerPinar GumusNejat NizamNurcan BuduneliMarvin WhiteleyAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Peter Jorth Keith H. Turner Pinar Gumus Nejat Nizam Nurcan Buduneli Marvin Whiteley Metatranscriptomics of the Human Oral Microbiome during Health and Disease
description	ABSTRACT The human microbiome plays important roles in health, but when disrupted, these same indigenous microbes can cause disease. The composition of the microbiome changes during the transition from health to disease; however, these changes are often not conserved among patients. Since microbiome-associated diseases like periodontitis cause similar patient symptoms despite interpatient variability in microbial community composition, we hypothesized that human-associated microbial communities undergo conserved changes in metabolism during disease. Here, we used patient-matched healthy and diseased samples to compare gene expression of 160,000 genes in healthy and diseased periodontal communities. We show that health- and disease-associated communities exhibit defined differences in metabolism that are conserved between patients. In contrast, the metabolic gene expression of individual species was highly variable between patients. These results demonstrate that despite high interpatient variability in microbial composition, disease-associated communities display conserved metabolic profiles that are generally accomplished by a patient-specific cohort of microbes. IMPORTANCE The human microbiome project has shown that shifts in our microbiota are associated with many diseases, including obesity, Crohn’s disease, diabetes, and periodontitis. While changes in microbial populations are apparent during these diseases, the species associated with each disease can vary from patient to patient. Taking into account this interpatient variability, we hypothesized that specific microbiota-associated diseases would be marked by conserved microbial community behaviors. Here, we use gene expression analyses of patient-matched healthy and diseased human periodontal plaque to show that microbial communities have highly conserved metabolic gene expression profiles, whereas individual species within the community do not. Furthermore, disease-associated communities exhibit conserved changes in metabolic and virulence gene expression.
format	article
author	Peter Jorth Keith H. Turner Pinar Gumus Nejat Nizam Nurcan Buduneli Marvin Whiteley
author_facet	Peter Jorth Keith H. Turner Pinar Gumus Nejat Nizam Nurcan Buduneli Marvin Whiteley
author_sort	Peter Jorth
title	Metatranscriptomics of the Human Oral Microbiome during Health and Disease
title_short	Metatranscriptomics of the Human Oral Microbiome during Health and Disease
title_full	Metatranscriptomics of the Human Oral Microbiome during Health and Disease
title_fullStr	Metatranscriptomics of the Human Oral Microbiome during Health and Disease
title_full_unstemmed	Metatranscriptomics of the Human Oral Microbiome during Health and Disease
title_sort	metatranscriptomics of the human oral microbiome during health and disease
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/e1c6317a50144420a8414a6ccd9e6781
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Metatranscriptomics of the Human Oral Microbiome during Health and Disease

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