Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals

ABSTRACT The capacity to resist the bactericidal action of complement (C′) is a strong but poorly understood virulence trait in Klebsiella spp. Killing requires activation of one or more C′ pathways, assembly of C5b-9 membrane attack complexes (MACs) on the surface of the outer membrane (OM), and pe...

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Autores principales:	Jessica Loraine, Eva Heinz, Jessica De Sousa Almeida, Oleksandr Milevskyy, Supayang P. Voravuthikunchai, Potjanee Srimanote, Pattarachai Kiratisin, Nicholas R. Thomson, Peter W. Taylor
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Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Klebsiella pneumoniae complement resistance lipopolysaccharide polysaccharide capsules whole-genome sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e1cda7f719f248bd8f2faf90722ce603
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spelling	oai:doaj.org-article:e1cda7f719f248bd8f2faf90722ce6032021-11-15T15:22:21ZComplement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals10.1128/mSphere.00537-182379-5042https://doaj.org/article/e1cda7f719f248bd8f2faf90722ce6032018-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00537-18https://doaj.org/toc/2379-5042ABSTRACT The capacity to resist the bactericidal action of complement (C′) is a strong but poorly understood virulence trait in Klebsiella spp. Killing requires activation of one or more C′ pathways, assembly of C5b-9 membrane attack complexes (MACs) on the surface of the outer membrane (OM), and penetration of MACs into the target bilayer. We interrogated whole-genome sequences of 164 Klebsiella isolates from three tertiary hospitals in Thailand for genes encoding surface-located macromolecules considered to play a role in determination of C′ resistance. Most isolates (154/164) were identified as Klebsiella pneumoniae, and the collection conformed to previously established population structures and antibiotic resistance patterns. The distribution of sequence types (STs) and capsular (K) types were also typical of global populations. The majority (64%) of isolates were resistant to C′, and the remainder were either rapidly or slowly killed. All isolates carried genes encoding capsular polysaccharides (K antigens), which have been strongly linked to C′ resistance. In contrast to previous reports, there were no differences in the amount of capsule produced by C′-resistant isolates compared to C′-susceptible isolates, nor was there any correlation between serum reactivity and the presence of hypermucoviscous capsules. Similarly, there were no correlations between the presence of genes specifying lipopolysaccharide O-side chains or major OM proteins. Some virulence factors were found more frequently in C′-resistant isolates but were considered to reflect clonal ST expansion. Thus, no single gene accounts for the C′ resistance of the isolates sequenced in this study. IMPORTANCE Multidrug-resistant Klebsiella pneumoniae is responsible for an increasing proportion of nosocomial infections, and emerging hypervirulent K. pneumoniae clones now cause severe community-acquired infections in otherwise healthy individuals. These bacteria are adept at circumventing immune defenses, and most survive and grow in serum; their capacity to avoid C′-mediated destruction is correlated with their invasive potential. Killing of Gram-negative bacteria occurs following activation of the C′ cascades and stable deposition of C5b-9 MACs onto the OM. For Klebsiella, studies with mutants and conjugants have invoked capsules, lipopolysaccharide O-side chains, and OM proteins as determinants of C′ resistance, although the precise roles of the macromolecules are unclear. In this study, we sequenced 164 Klebsiella isolates with different C′ susceptibilities to identify genes involved in resistance. We conclude that no single OM constituent can account for resistance, which is likely to depend on biophysical properties of the target bilayer.Jessica LoraineEva HeinzJessica De Sousa AlmeidaOleksandr MilevskyySupayang P. VoravuthikunchaiPotjanee SrimanotePattarachai KiratisinNicholas R. ThomsonPeter W. TaylorAmerican Society for MicrobiologyarticleKlebsiella pneumoniaecomplement resistancelipopolysaccharidepolysaccharide capsuleswhole-genome sequencingMicrobiologyQR1-502ENmSphere, Vol 3, Iss 6 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumoniae complement resistance lipopolysaccharide polysaccharide capsules whole-genome sequencing Microbiology QR1-502
spellingShingle	Klebsiella pneumoniae complement resistance lipopolysaccharide polysaccharide capsules whole-genome sequencing Microbiology QR1-502 Jessica Loraine Eva Heinz Jessica De Sousa Almeida Oleksandr Milevskyy Supayang P. Voravuthikunchai Potjanee Srimanote Pattarachai Kiratisin Nicholas R. Thomson Peter W. Taylor Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals
description	ABSTRACT The capacity to resist the bactericidal action of complement (C′) is a strong but poorly understood virulence trait in Klebsiella spp. Killing requires activation of one or more C′ pathways, assembly of C5b-9 membrane attack complexes (MACs) on the surface of the outer membrane (OM), and penetration of MACs into the target bilayer. We interrogated whole-genome sequences of 164 Klebsiella isolates from three tertiary hospitals in Thailand for genes encoding surface-located macromolecules considered to play a role in determination of C′ resistance. Most isolates (154/164) were identified as Klebsiella pneumoniae, and the collection conformed to previously established population structures and antibiotic resistance patterns. The distribution of sequence types (STs) and capsular (K) types were also typical of global populations. The majority (64%) of isolates were resistant to C′, and the remainder were either rapidly or slowly killed. All isolates carried genes encoding capsular polysaccharides (K antigens), which have been strongly linked to C′ resistance. In contrast to previous reports, there were no differences in the amount of capsule produced by C′-resistant isolates compared to C′-susceptible isolates, nor was there any correlation between serum reactivity and the presence of hypermucoviscous capsules. Similarly, there were no correlations between the presence of genes specifying lipopolysaccharide O-side chains or major OM proteins. Some virulence factors were found more frequently in C′-resistant isolates but were considered to reflect clonal ST expansion. Thus, no single gene accounts for the C′ resistance of the isolates sequenced in this study. IMPORTANCE Multidrug-resistant Klebsiella pneumoniae is responsible for an increasing proportion of nosocomial infections, and emerging hypervirulent K. pneumoniae clones now cause severe community-acquired infections in otherwise healthy individuals. These bacteria are adept at circumventing immune defenses, and most survive and grow in serum; their capacity to avoid C′-mediated destruction is correlated with their invasive potential. Killing of Gram-negative bacteria occurs following activation of the C′ cascades and stable deposition of C5b-9 MACs onto the OM. For Klebsiella, studies with mutants and conjugants have invoked capsules, lipopolysaccharide O-side chains, and OM proteins as determinants of C′ resistance, although the precise roles of the macromolecules are unclear. In this study, we sequenced 164 Klebsiella isolates with different C′ susceptibilities to identify genes involved in resistance. We conclude that no single OM constituent can account for resistance, which is likely to depend on biophysical properties of the target bilayer.
format	article
author	Jessica Loraine Eva Heinz Jessica De Sousa Almeida Oleksandr Milevskyy Supayang P. Voravuthikunchai Potjanee Srimanote Pattarachai Kiratisin Nicholas R. Thomson Peter W. Taylor
author_facet	Jessica Loraine Eva Heinz Jessica De Sousa Almeida Oleksandr Milevskyy Supayang P. Voravuthikunchai Potjanee Srimanote Pattarachai Kiratisin Nicholas R. Thomson Peter W. Taylor
author_sort	Jessica Loraine
title	Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals
title_short	Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals
title_full	Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals
title_fullStr	Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals
title_full_unstemmed	Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals
title_sort	complement susceptibility in relation to genome sequence of recent <named-content content-type="genus-species">klebsiella pneumoniae</named-content> isolates from thai hospitals
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/e1cda7f719f248bd8f2faf90722ce603
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Complement Susceptibility in Relation to Genome Sequence of Recent <named-content content-type="genus-species">Klebsiella pneumoniae</named-content> Isolates from Thai Hospitals

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