Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Fluorescence imaging modalities are currently a routine tool for the assessment of marker distribution within biological tissues, including monitoring of fluorescent photosensitizers (PSs) in photodynamic therapy (PDT). Conventional fluorescence imaging techniques provide en-face two-dimensional ima...

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Autores principales:	Mikhail Kirillin, Aleksandr Khilov, Daria Kurakina, Anna Orlova, Valeriya Perekatova, Veronika Shishkova, Alfia Malygina, Anna Mironycheva, Irena Shlivko, Sergey Gamayunov, Ilya Turchin, Ekaterina Sergeeva
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	photodynamic therapy dual-wavelength fluorescence imaging Monte Carlo simulations chlorin-based photosensitizers light transport optical phantoms Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/e1d1ed4df8ba479f8fdee2ddb8b4b094
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spelling	oai:doaj.org-article:e1d1ed4df8ba479f8fdee2ddb8b4b0942021-11-25T17:04:17ZDual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies10.3390/cancers132258072072-6694https://doaj.org/article/e1d1ed4df8ba479f8fdee2ddb8b4b0942021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5807https://doaj.org/toc/2072-6694Fluorescence imaging modalities are currently a routine tool for the assessment of marker distribution within biological tissues, including monitoring of fluorescent photosensitizers (PSs) in photodynamic therapy (PDT). Conventional fluorescence imaging techniques provide en-face two-dimensional images, while depth-resolved techniques require complicated tomographic modalities. In this paper, we report on a cost-effective approach for the estimation of fluorophore localization depth based on dual-wavelength probing. Owing to significant difference in optical properties of superficial biotissues for red and blue ranges of optical spectra, simultaneous detection of fluorescence excited at different wavelengths provides complementary information from different measurement volumes. Here, we report analytical and numerical models of the dual-wavelength fluorescence imaging of PS-containing biotissues considering topical and intravenous PS administration, and demonstrate the feasibility of this approach for evaluation of the PS localization depth based on the fluorescence signal ratio. The results of analytical and numerical simulations, as well as phantom experiments, were translated to the in vivo imaging to interpret experimental observations in animal experiments, human volunteers, and clinical studies. The proposed approach allowed us to estimate typical accumulation depths of PS localization which are consistent with the morphologically expected values for both topical PS administration and intravenous injection.Mikhail KirillinAleksandr KhilovDaria KurakinaAnna OrlovaValeriya PerekatovaVeronika ShishkovaAlfia MalyginaAnna MironychevaIrena ShlivkoSergey GamayunovIlya TurchinEkaterina SergeevaMDPI AGarticlephotodynamic therapydual-wavelength fluorescence imagingMonte Carlo simulationschlorin-based photosensitizerslight transportoptical phantomsNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5807, p 5807 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	photodynamic therapy dual-wavelength fluorescence imaging Monte Carlo simulations chlorin-based photosensitizers light transport optical phantoms Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	photodynamic therapy dual-wavelength fluorescence imaging Monte Carlo simulations chlorin-based photosensitizers light transport optical phantoms Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Mikhail Kirillin Aleksandr Khilov Daria Kurakina Anna Orlova Valeriya Perekatova Veronika Shishkova Alfia Malygina Anna Mironycheva Irena Shlivko Sergey Gamayunov Ilya Turchin Ekaterina Sergeeva Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies
description	Fluorescence imaging modalities are currently a routine tool for the assessment of marker distribution within biological tissues, including monitoring of fluorescent photosensitizers (PSs) in photodynamic therapy (PDT). Conventional fluorescence imaging techniques provide en-face two-dimensional images, while depth-resolved techniques require complicated tomographic modalities. In this paper, we report on a cost-effective approach for the estimation of fluorophore localization depth based on dual-wavelength probing. Owing to significant difference in optical properties of superficial biotissues for red and blue ranges of optical spectra, simultaneous detection of fluorescence excited at different wavelengths provides complementary information from different measurement volumes. Here, we report analytical and numerical models of the dual-wavelength fluorescence imaging of PS-containing biotissues considering topical and intravenous PS administration, and demonstrate the feasibility of this approach for evaluation of the PS localization depth based on the fluorescence signal ratio. The results of analytical and numerical simulations, as well as phantom experiments, were translated to the in vivo imaging to interpret experimental observations in animal experiments, human volunteers, and clinical studies. The proposed approach allowed us to estimate typical accumulation depths of PS localization which are consistent with the morphologically expected values for both topical PS administration and intravenous injection.
format	article
author	Mikhail Kirillin Aleksandr Khilov Daria Kurakina Anna Orlova Valeriya Perekatova Veronika Shishkova Alfia Malygina Anna Mironycheva Irena Shlivko Sergey Gamayunov Ilya Turchin Ekaterina Sergeeva
author_facet	Mikhail Kirillin Aleksandr Khilov Daria Kurakina Anna Orlova Valeriya Perekatova Veronika Shishkova Alfia Malygina Anna Mironycheva Irena Shlivko Sergey Gamayunov Ilya Turchin Ekaterina Sergeeva
author_sort	Mikhail Kirillin
title	Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies
title_short	Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies
title_full	Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies
title_fullStr	Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies
title_full_unstemmed	Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies
title_sort	dual-wavelength fluorescence monitoring of photodynamic therapy: from analytical models to clinical studies
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e1d1ed4df8ba479f8fdee2ddb8b4b094
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Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

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