Phenotype remodeling in neutrophilic granulocyte subsets CD64-CD32+CD16+CD11B+NG, CD64+CD32+CD16+CD11B+NG in de novo experimental model of viral-bacterial infection in vitro

A search for new targeted therapeutic strategies based on examining immunopathogenetic mechanisms for emerging co-infections is relevant and may further contribute not only to optimizing choice of immunotropic drugs, but also to achieving positive clinical and immunological remission for abnormal in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	I. V. Nesterova, G. A. Chudilova, T. V. Rusinova, V. N. Pavlenko, Ya. A. Yutskevich, N. K. Barova, V. A. Tarakanov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2021
Materias:	neutrophilic granulocytes phenotype subpopulation coinfection experimental model fmlp dsrna Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/e1d725e021df44d6b7afb4e50f7ef90f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e1d725e021df44d6b7afb4e50f7ef90f
record_format	dspace
spelling	oai:doaj.org-article:e1d725e021df44d6b7afb4e50f7ef90f2021-11-22T07:09:55ZPhenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>2220-76192313-739810.15789/2220-7619-ROT-1517https://doaj.org/article/e1d725e021df44d6b7afb4e50f7ef90f2021-02-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1517https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398A search for new targeted therapeutic strategies based on examining immunopathogenetic mechanisms for emerging co-infections is relevant and may further contribute not only to optimizing choice of immunotropic drugs, but also to achieving positive clinical and immunological remission for abnormal infectious processes. Previously, our studies found that recurrent viral-bacterial respiratory infections are associated with dysfunction of neutrophilic granulocytes (NG) with varying degree of intensity in altered effector properties. NG dysfunctions are often associated with diverse phenotypic profiles characterized by varying density for expression level of functionally significant trigger receptors. The aim of the study was to pinpoint phenotype transformation in CD64-CD32+CD16+CD11b+, CD64+CD32+CD16+CD11b+ neutrophilic granulocytes in experimental model of viral-bacterial infection in vitro. We examined 52 peripheral blood samples collected from 13 healthy adult volunteers. Viral, bacterial and virus-bacterial infection was modelled in vitro by incubating blood-derived cell samples with formyl-methionyl-leucyl-phenylalanine (fMLP), double-stranded RNA (dsRNA) or in combination followed by assessing changes in immunophenotyping of CD64-CD32+CD16+CD11b+NG, CD64+CD32+CD16+CD11b+NG by using using MAbs CD16-ECD, CD64-FITC, CD32-PE, CD11b-PC5 conjugates (Beckman Coulter International SA, France). It was demonstrated that NGs from healthy adult volunteers were dominated by CD64-CD32+CD16+CD11b+NG as well as minor subset СD64+CD32+CD16+CD11b+ NG varying in expression density of membrane molecules. Percentage of the minor subset СD64+CD16+CD32+CD11b+ NG was significantly increased after exposure with dsRNA, fMLP and dsRNA+fMLP compared to untreated samples. Comparative analysis revealed that various immunotropic agents differed in affecting expression of surface receptor molecules CD16, CD32 and unidirectional effects, but of varying magnitude altering CD11b marker both in major and minor subsets. Preincubation with dsRNA followed by adding fMLP allowed to find that they co-stimulated expression of surface receptors in both NG subsets. We generated an experimental model of viral-bacterial co-infection in vitro by using fMLP and dsRNA and observed types of phenotype transformation in CD64-CD32+CD16+CD11b+ NG and CD64+CD32+CD16+CD11b+ NG subsets. This model can be used to evaluate transformation of other NG subset phenotypes, NG functional activity, features of NET formation as well as impact of various immunotropic agents on NG.I. V. NesterovaG. A. ChudilovaT. V. RusinovaV. N. PavlenkoYa. A. YutskevichN. K. BarovaV. A. TarakanovSankt-Peterburg : NIIÈM imeni Pasteraarticleneutrophilic granulocytesphenotypesubpopulationcoinfectionexperimental modelfmlpdsrnaInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 11, Iss 1, Pp 101-110 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	neutrophilic granulocytes phenotype subpopulation coinfection experimental model fmlp dsrna Infectious and parasitic diseases RC109-216
spellingShingle	neutrophilic granulocytes phenotype subpopulation coinfection experimental model fmlp dsrna Infectious and parasitic diseases RC109-216 I. V. Nesterova G. A. Chudilova T. V. Rusinova V. N. Pavlenko Ya. A. Yutskevich N. K. Barova V. A. Tarakanov Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
description	A search for new targeted therapeutic strategies based on examining immunopathogenetic mechanisms for emerging co-infections is relevant and may further contribute not only to optimizing choice of immunotropic drugs, but also to achieving positive clinical and immunological remission for abnormal infectious processes. Previously, our studies found that recurrent viral-bacterial respiratory infections are associated with dysfunction of neutrophilic granulocytes (NG) with varying degree of intensity in altered effector properties. NG dysfunctions are often associated with diverse phenotypic profiles characterized by varying density for expression level of functionally significant trigger receptors. The aim of the study was to pinpoint phenotype transformation in CD64-CD32+CD16+CD11b+, CD64+CD32+CD16+CD11b+ neutrophilic granulocytes in experimental model of viral-bacterial infection in vitro. We examined 52 peripheral blood samples collected from 13 healthy adult volunteers. Viral, bacterial and virus-bacterial infection was modelled in vitro by incubating blood-derived cell samples with formyl-methionyl-leucyl-phenylalanine (fMLP), double-stranded RNA (dsRNA) or in combination followed by assessing changes in immunophenotyping of CD64-CD32+CD16+CD11b+NG, CD64+CD32+CD16+CD11b+NG by using using MAbs CD16-ECD, CD64-FITC, CD32-PE, CD11b-PC5 conjugates (Beckman Coulter International SA, France). It was demonstrated that NGs from healthy adult volunteers were dominated by CD64-CD32+CD16+CD11b+NG as well as minor subset СD64+CD32+CD16+CD11b+ NG varying in expression density of membrane molecules. Percentage of the minor subset СD64+CD16+CD32+CD11b+ NG was significantly increased after exposure with dsRNA, fMLP and dsRNA+fMLP compared to untreated samples. Comparative analysis revealed that various immunotropic agents differed in affecting expression of surface receptor molecules CD16, CD32 and unidirectional effects, but of varying magnitude altering CD11b marker both in major and minor subsets. Preincubation with dsRNA followed by adding fMLP allowed to find that they co-stimulated expression of surface receptors in both NG subsets. We generated an experimental model of viral-bacterial co-infection in vitro by using fMLP and dsRNA and observed types of phenotype transformation in CD64-CD32+CD16+CD11b+ NG and CD64+CD32+CD16+CD11b+ NG subsets. This model can be used to evaluate transformation of other NG subset phenotypes, NG functional activity, features of NET formation as well as impact of various immunotropic agents on NG.
format	article
author	I. V. Nesterova G. A. Chudilova T. V. Rusinova V. N. Pavlenko Ya. A. Yutskevich N. K. Barova V. A. Tarakanov
author_facet	I. V. Nesterova G. A. Chudilova T. V. Rusinova V. N. Pavlenko Ya. A. Yutskevich N. K. Barova V. A. Tarakanov
author_sort	I. V. Nesterova
title	Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
title_short	Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
title_full	Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
title_fullStr	Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
title_full_unstemmed	Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
title_sort	phenotype remodeling in neutrophilic granulocyte subsets cd64<sup>-</sup>cd32<sup>+</sup>cd16<sup>+</sup>cd11b<sup>+</sup>ng, cd64<sup>+</sup>cd32<sup>+</sup>cd16<sup>+</sup>cd11b<sup>+</sup>ng in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2021
url	https://doaj.org/article/e1d725e021df44d6b7afb4e50f7ef90f
work_keys_str_mv	AT ivnesterova phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi AT gachudilova phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi AT tvrusinova phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi AT vnpavlenko phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi AT yaayutskevich phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi AT nkbarova phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi AT vatarakanov phenotyperemodelinginneutrophilicgranulocytesubsetscd64supsupcd32supsupcd16supsupcd11bsupsupngcd64supsupcd32supsupcd16supsupcd11bsupsupnginidenovoiexperimentalmodelofviralbacterialinfectioniinvitroi
_version_	1718417887717228544

Phenotype remodeling in neutrophilic granulocyte subsets CD64<sup>-</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG, CD64<sup>+</sup>CD32<sup>+</sup>CD16<sup>+</sup>CD11B<sup>+</sup>NG in <i>de novo</i> experimental model of viral-bacterial infection <i>in vitro</i>

Ejemplares similares