A general approach to visualize protein binding and DNA conformation without protein labelling

Single-molecule imaging of protein-DNA association requires fluorescently labelled protein, which limits the protein concentration that can be used. Here the authors exploit protein induced fluorescent enhancement of DNA sparsely labelled with Cy3 to visualize protein binding and correlate it with c...

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Autores principales:	Dan Song, Thomas G. W. Graham, Joseph J. Loparo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e1e0b846b15649ecbc5a25f8158812ab
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spelling	oai:doaj.org-article:e1e0b846b15649ecbc5a25f8158812ab2021-12-02T17:31:49ZA general approach to visualize protein binding and DNA conformation without protein labelling10.1038/ncomms109762041-1723https://doaj.org/article/e1e0b846b15649ecbc5a25f8158812ab2016-03-01T00:00:00Zhttps://doi.org/10.1038/ncomms10976https://doaj.org/toc/2041-1723Single-molecule imaging of protein-DNA association requires fluorescently labelled protein, which limits the protein concentration that can be used. Here the authors exploit protein induced fluorescent enhancement of DNA sparsely labelled with Cy3 to visualize protein binding and correlate it with changes in DNA conformation.Dan SongThomas G. W. GrahamJoseph J. LoparoNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-7 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Dan Song Thomas G. W. Graham Joseph J. Loparo A general approach to visualize protein binding and DNA conformation without protein labelling
description	Single-molecule imaging of protein-DNA association requires fluorescently labelled protein, which limits the protein concentration that can be used. Here the authors exploit protein induced fluorescent enhancement of DNA sparsely labelled with Cy3 to visualize protein binding and correlate it with changes in DNA conformation.
format	article
author	Dan Song Thomas G. W. Graham Joseph J. Loparo
author_facet	Dan Song Thomas G. W. Graham Joseph J. Loparo
author_sort	Dan Song
title	A general approach to visualize protein binding and DNA conformation without protein labelling
title_short	A general approach to visualize protein binding and DNA conformation without protein labelling
title_full	A general approach to visualize protein binding and DNA conformation without protein labelling
title_fullStr	A general approach to visualize protein binding and DNA conformation without protein labelling
title_full_unstemmed	A general approach to visualize protein binding and DNA conformation without protein labelling
title_sort	general approach to visualize protein binding and dna conformation without protein labelling
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/e1e0b846b15649ecbc5a25f8158812ab
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A general approach to visualize protein binding and DNA conformation without protein labelling

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