A general approach to visualize protein binding and DNA conformation without protein labelling
Single-molecule imaging of protein-DNA association requires fluorescently labelled protein, which limits the protein concentration that can be used. Here the authors exploit protein induced fluorescent enhancement of DNA sparsely labelled with Cy3 to visualize protein binding and correlate it with c...
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Autores principales: | Dan Song, Thomas G. W. Graham, Joseph J. Loparo |
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Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2016
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Materias: | |
Acceso en línea: | https://doaj.org/article/e1e0b846b15649ecbc5a25f8158812ab |
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